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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126SubstrateUniRule annotation1
Binding sitei187SubstrateUniRule annotation1
Active sitei188Proton donor/acceptorUniRule annotation1
Active sitei318UniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Sitei331Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:YPO2264, y2106, YP_2061
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001613311 – 465Fumarate hydratase class IIAdd BLAST465

Proteomic databases

PaxDbiQ8ZEB6
PRIDEiQ8ZEB6

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y2106

Structurei

3D structure databases

ProteinModelPortaliQ8ZEB6
SMRiQ8ZEB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Substrate bindingUniRule annotation3
Regioni129 – 132Substrate binding (B site)UniRule annotation4
Regioni139 – 141Substrate bindingUniRule annotation3
Regioni324 – 326Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA
HOGENOMiHOG000061736
KOiK01679
OMAiVSFTDNC

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.101 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZEB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTRSEKDS MGSIDVPANQ LWGAQTQRSL AHFRISQEKM PTELIHALAL
60 70 80 90 100
TKRAAAQVNM DLGLLPAERA KAIMRAADEV LDGAHPTEFP LAIWQTGSGT
110 120 130 140 150
QTNMNMNEVL ANRASELLGG ARGNNRLVHP NDDVNKSQSS NDVFPTAMHV
160 170 180 190 200
AAVMGVSEHL LPELKVLQKT LADKAEAYRD IVKIGRTHLQ DATPLTLGQE
210 220 230 240 250
ISGWAAMLSH SVRHIEATLP HLCELALGGT AVGTGLNTHP EYAVRVANEI
260 270 280 290 300
ATLTRQPFIT APNKFESLGT CDALVHGHGA LKGLAASLMK IANDVRWLSS
310 320 330 340 350
GPRCGIGEIS IPENEPGSSI MPGKVNPTQC EAMTMLCAQV MGNDVAINIG
360 370 380 390 400
GASGNFELNV FRPLVIHNFL QSVRLLADGM RGFNEHCALG IEPNRDRITQ
410 420 430 440 450
LLNESLMLVT ALNTHIGYDK AAEIAKKAHK EGLTLKAAAM ALGYLTDAEF
460
DEWVRPEDMV GSMKK
Length:465
Mass (Da):50,129
Last modified:March 1, 2002 - v1
Checksum:iACCDACBCD38851A7
GO

Sequence cautioni

The sequence AAS62274 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL20891.1
AE009952 Genomic DNA Translation: AAM85669.1
AE017042 Genomic DNA Translation: AAS62274.1 Different initiation.
PIRiAH0275
RefSeqiWP_002210578.1, NZ_PDBR01000001.1
YP_008853946.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM85669; AAM85669; y2106
AAS62274; AAS62274; YP_2061
GeneIDi17699570
KEGGiype:YPO2263a
ypk:y2106
ypm:YP_2061

Similar proteinsi

Entry informationi

Entry nameiFUMC_YERPE
AccessioniPrimary (citable) accession number: Q8ZEB6
Secondary accession number(s): Q0WEQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome