Reviewed, UniProtKB/Swiss-Prot Q8ZD80 (NADB_YERPE)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	YPO2710, y1289, YP_2514

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	L-aspartate oxidase		PRO_0000184406
Regions
	Nucleotide binding	12 – 26	15	FAD Potential
Sites
	Active site	244	1	By similarity
	Active site	263	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZD80-1 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 909E8BFEB15BD2BD	FASTA	533	59,294
	10 20 30 40 50 60 MQSSSEHISD VLIIGSGAAG LSLALRLAPH CKVTVLSKGP LNEGATFYAQ GGIAAVFDET 70 80 90 100 110 120 DSISSHVDDT LIAGAGLCDK EAVEFIASNA RSCVQWLIDQ GVLFDTETSA NGEERYHLTR 130 140 150 160 170 180 EGGHSHRRIL HTADATGKEV ETTLVGKASN HPNISVKERC NAVDLITSNK IGLAGTKRVV 190 200 210 220 230 240 GAYVWNRELE KVETFRAKAV VLATGGAAKV YQYTTNPDIS SGDGIAMAWR AGCRVANLEF 250 260 270 280 290 300 NQFHPTCLFH PQARNFLLTE ALRGEGAYLK RPDGSRFMLD FDPRGELAPR DIVARAIDHE 310 320 330 340 350 360 MKRLGADCMY LDISHKPTDF VMQHFPMIYE KLLSLGIDLT KEAIPIVPAA HYTCGGVMVD 370 380 390 400 410 420 QHGRTDLDGL YAIGEVSYTG LHGANRMASN SLLECLVYGW SAAEDILTRL PTAKLAKHLP 430 440 450 460 470 480 DWDESRVDNS DERVVIQHNW HELRLFMWDY VGIVRTTKRL ERALRRINTL QQEIDEYYAN 490 500 510 520 530 FRISNNLLEL RNLVQVAELI VRCAMERKES RGLHYTLDYP DQIENPQPTI LHP

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590842 Genomic DNA. Translation: CAL21329.1. Different initiation. AE009952 Genomic DNA. Translation: AAM84863.1. Different initiation. AE017042 Genomic DNA. Translation: AAS62712.1. Different initiation.
PIR	AF0330.
RefSeq	NP_668612.1. NP_993835.1. YP_002347657.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
SMR	Q8ZD80. Positions 6-533.
ModBase	Search...
Genome annotation databases
GeneID	1146236. 1175540. 2764388.
GenomeReviews	Gene locus y1289 in contig AE009952_GR. Gene locus YP_2514 in contig AE017042_GR. Gene locus YPO2710 in contig AL590842_GR.
KEGG	ype:YPO2710. ypk:y1289. ypm:YP_2514.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZD80.
Enzyme and pathway databases
BioCyc	YPES187410:Y1289-MON. YPES214092:YPO2710-MON. YPES229193:YP2514-MON.
BRENDA	1.4.3.16. 142588.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_YERPE

Accession

Primary (citable) accession number: Q8ZD80
Secondary accession number(s): Q0WDI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	August 30, 2002
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)