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Q8ZD74 (LEPA_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Elongation factor 4
Short name=EF-4
EC=3.6.5.n1
Alternative name(s):
Ribosomal back-translocase LepA
Gene names
Name:lepA
Ordered Locus Names:YPO2716, y1295, YP_2520
OrganismYersinia pestis [Reference proteome] [HAMAP]
Taxonomic identifier632 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071

Catalytic activity

GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071

Subcellular location

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 599599Elongation factor 4 HAMAP-Rule MF_00071
PRO_0000176381

Regions

Nucleotide binding14 – 196GTP By similarity
Nucleotide binding131 – 1344GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ZD74 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: BAF32CD792DB5BDB

FASTA59966,709
        10         20         30         40         50         60 
MKHIRNFSII AHIDHGKSTL SDRIIQICGG LSEREMAAQV LDSMDLERER GITIKAQSVT 

        70         80         90        100        110        120 
LDYHSKDGQT YQLNFIDTPG HVDFSYEVSR SLAACEGALL VVDAGQGVEA QTLANCYTAM 

       130        140        150        160        170        180 
EMDLEVVPVL NKIDLPAADP ERVAEEIEDI VGIDATDAIR CSAKTGVGVP DVLERLVRDI 

       190        200        210        220        230        240 
PAPEGDPNGP LQALIIDSWF DNYLGVVSLI RIKNGSLRKG DKVKVMSTGQ SYNADRLGIF 

       250        260        270        280        290        300 
TPKRVDRDVL NCGEVGWLVC AIKDILGAPV GDTLTLTRNP AEKSLPGFKK VKPQVYAGLF 

       310        320        330        340        350        360 
PISSDDYESF RDALGKLSLN DASLFYEPES STALGFGFRC GFLGLLHMEI IQERLEREYD 

       370        380        390        400        410        420 
LELITTAPTV VYEVITTNQE TVYVDSPSKL PALNNIEELR EPIAECHMLL PQEYLGNVIT 

       430        440        450        460        470        480 
LCIEKRGTQT NMVYHGKQVA LTYEIPMAEV VLDFFDRLKS TSRGYASLDY NFKRFQTSDM 

       490        500        510        520        530        540 
VRVDVLINNE RVDALALITH RDNAQYRGRD LVEKMKELIP RQQFDIAIQA AIGNHIIARS 

       550        560        570        580        590 
TVKQLRKNVL AKCYGGDVSR KKKLLQKQKD GKKRMKQVGN VELPQEAFLA ILHVGKDSK

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References

[1]"Genome sequence of Yersinia pestis, the causative agent of plague."
Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. expand/collapse author list , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.
Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CO-92 / Biovar Orientalis.
[2]"Genome sequence of Yersinia pestis KIM."
Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. expand/collapse author list , Matson J.S., Blattner F.R., Perry R.D.
J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KIM10+ / Biovar Mediaevalis.
[3]"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. expand/collapse author list , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL590842 Genomic DNA. Translation: CAL21335.1.
AE009952 Genomic DNA. Translation: AAM84869.1.
AE017042 Genomic DNA. Translation: AAS62718.1.
PIRAD0331.
RefSeqNP_668618.1. NC_004088.1.
NP_993841.1. NC_005810.1.
YP_002347663.1. NC_003143.1.

3D structure databases

ProteinModelPortalQ8ZD74.
SMRQ8ZD74. Positions 1-555.
ModBaseSearch...

Protein-protein interaction databases

STRING214092.YPO2716.

Proteomic databases

PRIDEQ8ZD74.

Protocols and materials databases

DNASU1146242.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM84869; AAM84869; y1295.
AAS62718; AAS62718; YP_2520.
GeneID1146242.
1175546.
2763941.
KEGGype:YPO2716.
ypk:y1295.
ypm:YP_2520.

Phylogenomic databases

eggNOGCOG0481.
HOGENOMHOG000020624.
KOK03596.
OMAKCYGGDA.
ProtClustDBPRK05433.

Family and domain databases

Gene3D3.30.70.240. 1 hit.
HAMAPMF_00071. LepA.
InterProIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR009000. Transl_elong_init/rib_B-barrel.
[Graphical view]
PANTHERPTHR23115:SF40. PTHR23115:SF40. 1 hit.
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF54980. EFG_III_V. 2 hits.
SSF50447. Translat_factor. 1 hit.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPA_YERPE
AccessionPrimary (citable) accession number: Q8ZD74
Secondary accession number(s): Q0WDH5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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