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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei125Important for catalytic activityUniRule annotation1
Sitei147Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:YPO2747, y1580, YP_2417
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001093151 – 747Fatty acid oxidation complex subunit alphaAdd BLAST747

Proteomic databases

PRIDEiQ8ZD45.

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi187410.y1580.

Structurei

3D structure databases

ProteinModelPortaliQ8ZD45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 197Enoyl-CoA hydrataseUniRule annotationAdd BLAST190
Regioni313 – 7413-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST429

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT. Bacteria.
COG1024. LUCA.
COG1250. LUCA.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZD45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGASATNSVT HPAFTLNVRP DNIGIITIDV VGDKVNTLKA EFADQIATIL
60 70 80 90 100
QQAHALPKLQ GLVIVSGKPD SFIAGADITM IAACRTAHDA RVLAQKGQSI
110 120 130 140 150
LAQIAAFPVP VVAAIHGACL GGGLELALAC HSRICSLDDK TVLGLPEVQL
160 170 180 190 200
GLLPGSGGTQ RLPRLVGVSK ALDMILTGKQ IRPRQALKMG LVDDVVPRDI
210 220 230 240 250
LLDVAIQRAK AGWLNRRALP WQERLLSGPL GKALLFRIVR KKTLAKTRGH
260 270 280 290 300
YPAAERIIDV VRKGLDQGGP SGYEAEARAF GELAMSPQSA ALRSLFFATT
310 320 330 340 350
SLKKETGSAA TARAIHRVGV LGGGLMGGGI ANVTATRAGL PVRIKDINPQ
360 370 380 390 400
GINQALKYTW DALGKRVRSK RMRPTEQQRQ MMLISGSTDY RGFERVDIVV
410 420 430 440 450
EAVFEDLSLK QQMVADIERF GAAHTIFASN TSSLPISQIA ALAQRPEQVI
460 470 480 490 500
GLHYFSPVDK MPLVEVIPHE KTSEETIATT VALARKQGKT AIVVADRAGF
510 520 530 540 550
YVNRILAPYI NEAARCLLDG EPIESVDNAL VDFGFPVGPM MLLDEVGIDV
560 570 580 590 600
ATKIMPILVE QLGPRFAAPP SFDVILKDGR KGRKNGRGFY LYSNPTKNSS
610 620 630 640 650
PTKNGNSPAK RNSFKWRKNK VKPVDASIYT LLGVTPKAHL GAGVITQRCT
660 670 680 690 700
MLMLNEAVRC LDESIIRNPR DGDIGAVFGI GFPPFLGGPF RYLDSLGADK
710 720 730 740
VVQALRLLVQ QYGERFEPCQ RLVTMAEQQQ QFYPVDANID EVTDVAS
Length:747
Mass (Da):80,695
Last modified:December 6, 2005 - v2
Checksum:iB6D95C35353F458F
GO

Sequence cautioni

The sequence AAM85149 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS62622 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAL21366 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21366.1. Different initiation.
AE009952 Genomic DNA. Translation: AAM85149.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS62622.1. Different initiation.
PIRiAC0335.
RefSeqiYP_002347694.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM85149; AAM85149; y1580.
AAS62622; AAS62622; YP_2417.
GeneIDi1175578.
KEGGiype:YPO2747.
ypk:y1580.
ypm:YP_2417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21366.1. Different initiation.
AE009952 Genomic DNA. Translation: AAM85149.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS62622.1. Different initiation.
PIRiAC0335.
RefSeqiYP_002347694.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ8ZD45.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y1580.

Proteomic databases

PRIDEiQ8ZD45.

Protocols and materials databases

DNASUi1146527.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM85149; AAM85149; y1580.
AAS62622; AAS62622; YP_2417.
GeneIDi1175578.
KEGGiype:YPO2747.
ypk:y1580.
ypm:YP_2417.

Phylogenomic databases

eggNOGiENOG4105DYT. Bacteria.
COG1024. LUCA.
COG1250. LUCA.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.

Enzyme and pathway databases

UniPathwayiUPA00659.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFADJ_YERPE
AccessioniPrimary (citable) accession number: Q8ZD45
Secondary accession number(s): Q0WDE4, Q74SY6, Q8D0U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.