Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8ZD45

- FADJ_YERPE

UniProt

Q8ZD45 - FADJ_YERPE

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Yersinia pestis
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei125 – 1251Important for catalytic activityUniRule annotation
    Sitei147 – 1471Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciYPES214092:GKDD-2716-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:YPO2747, y1580, YP_2417
    OrganismiYersinia pestis
    Taxonomic identifieri632 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 747747Fatty acid oxidation complex subunit alphaPRO_0000109315Add
    BLAST

    Proteomic databases

    PRIDEiQ8ZD45.

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi214092.YPO2747.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8ZD45.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 197190Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni313 – 7414293-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OMAiPFRYMDT.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8ZD45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGASATNSVT HPAFTLNVRP DNIGIITIDV VGDKVNTLKA EFADQIATIL    50
    QQAHALPKLQ GLVIVSGKPD SFIAGADITM IAACRTAHDA RVLAQKGQSI 100
    LAQIAAFPVP VVAAIHGACL GGGLELALAC HSRICSLDDK TVLGLPEVQL 150
    GLLPGSGGTQ RLPRLVGVSK ALDMILTGKQ IRPRQALKMG LVDDVVPRDI 200
    LLDVAIQRAK AGWLNRRALP WQERLLSGPL GKALLFRIVR KKTLAKTRGH 250
    YPAAERIIDV VRKGLDQGGP SGYEAEARAF GELAMSPQSA ALRSLFFATT 300
    SLKKETGSAA TARAIHRVGV LGGGLMGGGI ANVTATRAGL PVRIKDINPQ 350
    GINQALKYTW DALGKRVRSK RMRPTEQQRQ MMLISGSTDY RGFERVDIVV 400
    EAVFEDLSLK QQMVADIERF GAAHTIFASN TSSLPISQIA ALAQRPEQVI 450
    GLHYFSPVDK MPLVEVIPHE KTSEETIATT VALARKQGKT AIVVADRAGF 500
    YVNRILAPYI NEAARCLLDG EPIESVDNAL VDFGFPVGPM MLLDEVGIDV 550
    ATKIMPILVE QLGPRFAAPP SFDVILKDGR KGRKNGRGFY LYSNPTKNSS 600
    PTKNGNSPAK RNSFKWRKNK VKPVDASIYT LLGVTPKAHL GAGVITQRCT 650
    MLMLNEAVRC LDESIIRNPR DGDIGAVFGI GFPPFLGGPF RYLDSLGADK 700
    VVQALRLLVQ QYGERFEPCQ RLVTMAEQQQ QFYPVDANID EVTDVAS 747
    Length:747
    Mass (Da):80,695
    Last modified:December 6, 2005 - v2
    Checksum:iB6D95C35353F458F
    GO

    Sequence cautioni

    The sequence AAM85149.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAS62622.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAL21366.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL21366.1. Different initiation.
    AE009952 Genomic DNA. Translation: AAM85149.1. Different initiation.
    AE017042 Genomic DNA. Translation: AAS62622.1. Different initiation.
    PIRiAC0335.
    RefSeqiNP_668898.1. NC_004088.1.
    NP_993745.1. NC_005810.1.
    YP_002347694.1. NC_003143.1.

    Genome annotation databases

    EnsemblBacteriaiAAM85149; AAM85149; y1580.
    AAS62622; AAS62622; YP_2417.
    GeneIDi1146527.
    1175578.
    2764829.
    KEGGiype:YPO2747.
    ypk:y1580.
    ypm:YP_2417.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590842 Genomic DNA. Translation: CAL21366.1 . Different initiation.
    AE009952 Genomic DNA. Translation: AAM85149.1 . Different initiation.
    AE017042 Genomic DNA. Translation: AAS62622.1 . Different initiation.
    PIRi AC0335.
    RefSeqi NP_668898.1. NC_004088.1.
    NP_993745.1. NC_005810.1.
    YP_002347694.1. NC_003143.1.

    3D structure databases

    ProteinModelPortali Q8ZD45.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 214092.YPO2747.

    Proteomic databases

    PRIDEi Q8ZD45.

    Protocols and materials databases

    DNASUi 1146527.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM85149 ; AAM85149 ; y1580 .
    AAS62622 ; AAS62622 ; YP_2417 .
    GeneIDi 1146527.
    1175578.
    2764829.
    KEGGi ype:YPO2747.
    ypk:y1580.
    ypm:YP_2417.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OMAi PFRYMDT.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci YPES214092:GKDD-2716-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CO-92 / Biovar Orientalis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KIM10+ / Biovar Mediaevalis.
    3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
      Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
      , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
      DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 91001 / Biovar Mediaevalis.

    Entry informationi

    Entry nameiFADJ_YERPE
    AccessioniPrimary (citable) accession number: Q8ZD45
    Secondary accession number(s): Q0WDE4, Q74SY6, Q8D0U8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3