Q8ZCS5 (HSCA_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein HscA

Alternative name(s):
Hsc66

Gene names

Name:	hscA
Ordered Locus Names:	YPO2892, y1339, YP_2563

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB. Involved in the maturation of IscU By similarity. HAMAP-Rule MF_00679
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	iron-sulfur cluster assembly Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Chaperone protein HscA HAMAP-Rule MF_00679		PRO_0000078657
Regions
	Repeat	46 – 50	5	1 HAMAP-Rule MF_00679
	Repeat	51 – 56	6	2 HAMAP-Rule MF_00679
	Repeat	57 – 62	6	3 HAMAP-Rule MF_00679
	Repeat	63 – 68	6	4 HAMAP-Rule MF_00679
	Repeat	69 – 74	6	5 HAMAP-Rule MF_00679
	Repeat	75 – 79	5	6 HAMAP-Rule MF_00679
	Region	49 – 82	34	Insert HAMAP-Rule MF_00679
Natural variations
	Natural variant	46 – 50	5	Missing in strain: 91001.
	Natural variant	57 – 62	6	Missing in strain: KIM5.

Sequences

	Sequence		Length	Mass (Da)
	Q8ZCS5 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 398322578B9B7F3B	FASTA	650	69,210
	10 20 30 40 50 60 MALLQISEPG LTAAPHQRRL AAGIDLGTTN SLVATVRSGK AQTLADEQLA DEQLIADEQL 70 80 90 100 110 120 IAGEQLIAGQ QLIAGQQLIN EKQRDLLPSV VHYHPQGIDV GWKARNLAAL DPVNTISSVK 130 140 150 160 170 180 RMMGRSLADI VQRYPNLPYQ FHASENGLPM IQTARGLVNP VQVSAEILKT LAQRAQAALA 190 200 210 220 230 240 GELDGVVITV PAYFDDAQRQ GTKDAARLAG LHVLRLLNEP TAAAIAYGLD SGQEGVIAVY 250 260 270 280 290 300 DLGGGTFDIS ILRLSRGVFE VLATGGDSAL GGDDFDHLLA DWLREQAGVA TRDDHGIQRQ 310 320 330 340 350 360 LLDTAIAAKI ALSEAETAVV SVAGWQGEVT REQLESLIAP QVKRTLMACR RALKDAGVTA 370 380 390 400 410 420 DEILEVVMVG GSTRVPLVRE QVGQFFGRTP LTSIDPDKVV AIGAAIQADI LVGNKPDSDM 430 440 450 460 470 480 LLLDVIPLSL GLETMGGLVE KVIPRNTTIP AARAQEFTTF KDGQSAMMIH VLQGERELVK 490 500 510 520 530 540 DCRSLARFTL RGLPPLPAGG AHIRVTFQVD ADGLLSVTAM EKSTGVEASI QVKPSYGLSD 550 560 570 580 590 600 DEIANMIKDS MANAQSDITA RKLAEQRVDA ARVLESLQGA LAEDAALLSE QESAAIAQAV 610 620 630 640 650 ALLQQQMQGS DPQAIEAAIK ALDAQTQDFA ARRMDASIRR ALAGHSVDEV

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL21503.1. AE009952 Genomic DNA. Translation: AAM84912.1. AE017042 Genomic DNA. Translation: AAS62759.1.
PIR	AD0352.
RefSeq	NP_668661.1. NC_004088.1. NP_993882.1. NC_005810.1. YP_002347826.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZCS5.
SMR	Q8ZCS5. Positions 423-649.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8ZCS5. 2 interactions.
STRING	214092.YPO2892.
Proteomic databases
PRIDE	Q8ZCS5.
Protocols and materials databases
DNASU	1146286.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM84912; AAM84912; y1339. AAS62759; AAS62759; YP_2563.
GeneID	1146286. 1175716. 2764602.
KEGG	ype:YPO2892. ypk:y1339. ypm:YP_2563.
Phylogenomic databases
eggNOG	COG0443.
HOGENOM	HOG000228136.
KO	K04044.
OMA	VKPSFGL.
ProtClustDB	PRK05183.
Family and domain databases
HAMAP	MF_00679. HscA. Divergent sequence.
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. IPR010236. ISC_FeS_clus_asmbl_HscA. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR01991. HscA. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HSCA_YERPE

Accession

Primary (citable) accession number: Q8ZCS5
Secondary accession number(s): Q0WD12, Q8D0Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program