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Q8ZCQ2 (PUR4_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:YPO2921, y1309, YP_2536
OrganismYersinia pestis
Taxonomic identifier632 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length1296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12961296Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100428

Regions

Domain1043 – 1296254Glutamine amidotransferase type-1
Nucleotide binding306 – 31712ATP Potential

Sites

Active site11361Nucleophile By similarity
Active site12611 By similarity
Active site12631 By similarity

Experimental info

Sequence conflict281L → P Ref.2
Sequence conflict281L → P Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8ZCQ2 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 338EB52821A8888A

FASTA1,296142,048
        10         20         30         40         50         60 
MEILRGSPAL SAFRITKLLS RCQDAHLLVS DIYAEYVHFA DVSAPLSADE HARLQRLLQY 

        70         80         90        100        110        120 
GPSLPEHPPA GRLLLVTPRP GTISPWSSKA TDIAHNCGLS QILRLERGLA FSIQGPDLNE 

       130        140        150        160        170        180 
SQWKQLAALL HDRMMEAVFT DLQQAEQLFS HHQPAPVQRV DILGQGRSAL EQANIKLGLA 

       190        200        210        220        230        240 
LAQDEIDYLL TAFTGLGRNP TDIELYMFAQ ANSEHCRHKI FNADWVIDGV VQPKTLFKMI 

       250        260        270        280        290        300 
KNTFEHTPDY VLSAYKDNAA VMEGSQVGRF YATAEKGIYD YHQEEAHILM KVETHNHPTA 

       310        320        330        340        350        360 
ISPWPGAATG SGGEIRDEGA TGRGAKPKAG LVGFSVSNLR IPGFEQPWEE NFGKPDRIVT 

       370        380        390        400        410        420 
ALDIMTEGPL GGAAFNNEFG RPALLGYFRT YEERVNSHNG IELRGYHKPI MLAGGLGNIR 

       430        440        450        460        470        480 
ADHVQKGEIT VGAKLVVLGG PSMNIGLGGG AASSMASGQS DADLDFASVQ RDNPEMERRC 

       490        500        510        520        530        540 
QEVIDRCWQL GEYNPILFIH DVGAGGLSNA MPELVNDGGR GGRFELRDIL NDEPGMSPLE 

       550        560        570        580        590        600 
VWCNESQERY VLAVAPAQMA LFDEICRRER APYAVIGEAT EEKHLLLNDR HFGNQPIDMP 

       610        620        630        640        650        660 
LDVLLGKTPK MLRDVTRLQA KGDALQRADI SLAEAVKRIM HLPAVAEKTF LITIGDRTVT 

       670        680        690        700        710        720 
GMVTRDQMVG PWQIPVADCA VTSASLDSYY GEAMSLGERA PVALLDFAAS ARLAVGEALT 

       730        740        750        760        770        780 
NIAATQIGEL KRIKLSANWM SAAGHPGEDA GLYDAVRAVG EELCPALEIT IPVGKDSMSM 

       790        800        810        820        830        840 
KTRWQEGHEQ REMTSPLSLV ITAFARIEDV RRTVTPQLRT DKGDNALLLI DLGAGHNALG 

       850        860        870        880        890        900 
ATALTQVYRQ LGDKPADVRN VQQLAGFFNA MQRLVADQHL LAYHDRSDGG LLVTLAEMAF 

       910        920        930        940        950        960 
AGHCGVTVDI QSLGNDALAA LFNEELGAVI QVRAEQRADV EKLLADHGLA NCVHYLGRAV 

       970        980        990       1000       1010       1020 
AGDTFDIRSG TDVVYSEKRS TLRLWWAETS WQMQRLRDNP DCADQEHQAK QDESDPGLNV 

      1030       1040       1050       1060       1070       1080 
KLTFDPAEDI AAPFILKQAR PKVAVLREQG VNSHVEMAAA FHRAGFDAVD VHMSDLLAGR 

      1090       1100       1110       1120       1130       1140 
TDLQSFQTLV ACGGFSYGDV LGAGEGWAKS ILFNDRVRDE FEAFFHRPTT LALGVCNGCQ 

      1150       1160       1170       1180       1190       1200 
MMSNLRELIP GAEHWPRFVR NLSDSFEARF SLVEVASSPS LFMQDMVGSR MPIAVSHGEG 

      1210       1220       1230       1240       1250       1260 
QVEVRDAAHL AALEQSHLVA LRFVNNHGVV TEQYPANPNG SANGITAVTS VSGRATVMMP 

      1270       1280       1290 
HPERVFRTVS NSWHPEEWGE DSPWMRMFRN ARKQLG

« Hide

References

[1]"Genome sequence of Yersinia pestis, the causative agent of plague."
Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. expand/collapse author list , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.
Nature 413:523-527(2001) [PubMed: 11586360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CO-92 / Biovar Orientalis.
[2]"Genome sequence of Yersinia pestis KIM."
Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. expand/collapse author list , Matson J.S., Blattner F.R., Perry R.D.
J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KIM5 / Biovar Mediaevalis.
[3]"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. expand/collapse author list , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL590842 Genomic DNA. Translation: CAL21529.1.
AE009952 Genomic DNA. Translation: AAM84883.1.
AE017042 Genomic DNA. Translation: AAS62733.1.
PIRAF0355.
RefSeqNP_668632.1. NC_004088.1.
NP_993856.1. NC_005810.1.
YP_002347851.1. NC_003143.1.

3D structure databases

ProteinModelPortalQ8ZCQ2.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8ZCQ2. 10 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1146256.
1175742.
2766115.
GenomeReviewsGene locus y1309 in contig AE009952_GR.
Gene locus YP_2536 in contig AE017042_GR.
Gene locus YPO2921 in contig AL590842_GR.
KEGGype:YPO2921.
ypk:y1309.
ypm:YP_2536.

Phylogenomic databases

HOGENOMHBG335309.
OMAVKAVGME.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycYPES187410:Y1309-MONOMER.
YPES214092:YPO2921-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_YERPE
AccessionPrimary (citable) accession number: Q8ZCQ2
Secondary accession number(s): Q0WCY7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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