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Q8ZCF3 (NAPA_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:YPO3038, YP_2661
OrganismYersinia pestis [Reference proteome] [HAMAP]
Taxonomic identifier632 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length830 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 830799Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000046015

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict3411A → T in AAS62853. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8ZCF3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C83E1665F370EF6D

FASTA83093,226
        10         20         30         40         50         60 
MKLSRRDFMK ANAAVAAAAA AGMTIPTVAK AVGETTNAIK WDKAPCRFCG TGCGVLVGTQ 

        70         80         90        100        110        120 
NGRIVASQGD PDSPVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMKDGQYD KEGDFTPISW 

       130        140        150        160        170        180 
EKAFDIMELK FKNALKEKGP TAVGMFGSGQ WTVWEGYAAL KLLKGGFRSN NLDPNARHCM 

       190        200        210        220        230        240 
ASSVVGFMRT FGMDEPMGCY DDIEEADAFV LWGSNMAEMH PVLWSRMTSR RLTNAHVRIA 

       250        260        270        280        290        300 
VLSTYEHRSF ELADNPIVFT PQTDLVIMNY IANYIIQNNA VDKDFLAQHV NFRRGATDIG 

       310        320        330        340        350        360 
YGLRPTHPLE KAAKNPGSDA SEPMSFEDFK TFVAEYTLEK AAKMSGVPED QLESLAQLYA 

       370        380        390        400        410        420 
DPKVKLVSYW TMGFNQHTRG VWANNMCYNL HLLTGKISTP GSGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFSHRLPAD MVVTNEKHRQ IAETTWQLPA GTIPEKVGLH AVAQDRALKD GTLNAYWVMC 

       490        500        510        520        530        540 
NNNMQAGPNI NEERMPGWRD PRNFIVVSDP YPTISALSAD LILPTSMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVP SPGEAKSDLW QIVEFAKRFN VEEVWPAELV NQKPEYRGKN LYEVLFANDV 

       610        620        630        640        650        660 
VSKYPLSEIP DDQLNDEARD FGFYIQKGLF EEYASFGRGH AHDLAPFDVY HQVRGLRWPV 

       670        680        690        700        710        720 
VDGKETLWRY REGFDPFVPK GEEVRFYGKP DGKAVIFALP YEPAAESPDQ EYDLWLSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPEAVLFIHP LDAKARGLHR GDKVKVISRR GEVISLVETR 

       790        800        810        820        830 
GRNRPPRGLV YMPFFDAAQL VNNLTLDATD PLSKETDFKK CAVKLERVVA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590842 Genomic DNA. Translation: CAL21640.1.
AE017042 Genomic DNA. Translation: AAS62853.1.
PIRAE0369.
RefSeqNP_993976.1. NC_005810.1.
YP_002347958.1. NC_003143.1.

3D structure databases

ProteinModelPortalQ8ZCF3.
SMRQ8ZCF3. Positions 38-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING214092.YPO3038.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS62853; AAS62853; YP_2661.
GeneID1175859.
2766408.
KEGGype:YPO3038.
ypm:YP_2661.
PATRIC18595978. VBIYerPes7843_3685.

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAVITHSER.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycYPES214092:GKDD-2997-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_YERPE
AccessionPrimary (citable) accession number: Q8ZCF3
Secondary accession number(s): Q0WCN0, Q74SF1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families