Periplasmic nitrate reductase precursor

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Q8ZCF3 (NAPA_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 69. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	YPO3038, YP_2661

Organism

Yersinia pestis

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

Tat-type signal Potential

Chain

32 – 830

799

Periplasmic nitrate reductase HAMAP MF_01630

PRO_0000046015

Sites

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict

341

A → T in AAS62853. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q8ZCF3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C83E1665F370EF6D
Show »

FASTA

830

93,226

        10         20         30         40         50         60 
MKLSRRDFMK ANAAVAAAAA AGMTIPTVAK AVGETTNAIK WDKAPCRFCG TGCGVLVGTQ 

        70         80         90        100        110        120 
NGRIVASQGD PDSPVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMKDGQYD KEGDFTPISW 

       130        140        150        160        170        180 
EKAFDIMELK FKNALKEKGP TAVGMFGSGQ WTVWEGYAAL KLLKGGFRSN NLDPNARHCM 

       190        200        210        220        230        240 
ASSVVGFMRT FGMDEPMGCY DDIEEADAFV LWGSNMAEMH PVLWSRMTSR RLTNAHVRIA 

       250        260        270        280        290        300 
VLSTYEHRSF ELADNPIVFT PQTDLVIMNY IANYIIQNNA VDKDFLAQHV NFRRGATDIG 

       310        320        330        340        350        360 
YGLRPTHPLE KAAKNPGSDA SEPMSFEDFK TFVAEYTLEK AAKMSGVPED QLESLAQLYA 

       370        380        390        400        410        420 
DPKVKLVSYW TMGFNQHTRG VWANNMCYNL HLLTGKISTP GSGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFSHRLPAD MVVTNEKHRQ IAETTWQLPA GTIPEKVGLH AVAQDRALKD GTLNAYWVMC 

       490        500        510        520        530        540 
NNNMQAGPNI NEERMPGWRD PRNFIVVSDP YPTISALSAD LILPTSMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVP SPGEAKSDLW QIVEFAKRFN VEEVWPAELV NQKPEYRGKN LYEVLFANDV 

       610        620        630        640        650        660 
VSKYPLSEIP DDQLNDEARD FGFYIQKGLF EEYASFGRGH AHDLAPFDVY HQVRGLRWPV 

       670        680        690        700        710        720 
VDGKETLWRY REGFDPFVPK GEEVRFYGKP DGKAVIFALP YEPAAESPDQ EYDLWLSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPEAVLFIHP LDAKARGLHR GDKVKVISRR GEVISLVETR 

       790        800        810        820        830 
GRNRPPRGLV YMPFFDAAQL VNNLTLDATD PLSKETDFKK CAVKLERVVA

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References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL21640.1. AE017042 Genomic DNA. Translation: AAS62853.1.
PIR	AE0369.
RefSeq	NP_993976.1. NC_005810.1. YP_002347958.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZCF3.
SMR	Q8ZCF3. Positions 38-827.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1175859. 2766408.
GenomeReviews	Gene locus YP_2661 in contig AE017042_GR. Gene locus YPO3038 in contig AL590842_GR.
KEGG	ype:YPO3038. ypm:YP_2661.
PATRIC	18595978. VBIYerPes7843_3685.
Phylogenomic databases
HOGENOM	HBG715033.
OMA	NMLYNIH.
ProtClustDB	PRK13532.
Enzyme and pathway databases
BioCyc	YPES214092:YPO3038-MONOMER.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
KO	K02567.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_YERPE

Accession

Primary (citable) accession number: Q8ZCF3
Secondary accession number(s): Q0WCN0, Q74SF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents