Reviewed, UniProtKB/Swiss-Prot Q8ZCA7 (COPA_YERPE)

Names and origin

Protein names

Recommended name:
    Copper-exporting P-type ATPase A
    EC=3.6.3.n1

Gene names

Name:	copA
Ordered Locus Names:	YPO3086, y1093, YP_0838

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	961 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in copper export By similarity.
Catalytic activity	ATP + H(2)O + Cu(1+)(In) = ADP + phosphate + Cu(1+)(Out).
Subcellular location	Cell membrane; Multi-pass membrane proteinProbable.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily. Contains 3 HMA domains.

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	copper ion transport Inferred from electronic annotation. Source: UniProtKB-KW metabolic process Inferred from electronic annotation. Source: InterPro proton transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 961	961	Copper-exporting P-type ATPase A		PRO_0000046325
Regions
	Transmembrane	316 – 336	21	Potential
	Transmembrane	345 – 365	21	Potential
	Transmembrane	381 – 401	21	Potential
	Transmembrane	565 – 585	21	Potential
	Transmembrane	592 – 612	21	Potential
	Transmembrane	860 – 880	21	Potential
	Transmembrane	906 – 926	21	Potential
	Transmembrane	928 – 948	21	Potential
	Domain	4 – 65	62	HMA 1
	Domain	70 – 131	62	HMA 2
	Domain	227 – 290	64	HMA 3
Sites
	Active site	650	1	4-aspartylphosphate intermediate Probable
	Metal binding	14	1	Copper Potential
	Metal binding	17	1	Copper Potential
	Metal binding	80	1	Copper Potential
	Metal binding	83	1	Copper Potential
	Metal binding	237	1	Copper Potential
	Metal binding	240	1	Copper Potential
	Metal binding	847	1	Magnesium By similarity
	Metal binding	851	1	Magnesium By similarity
Experimental info
	Sequence conflict	376	1	R → S Ref.2 Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8ZCA7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 8FE8B194BF356A33	FASTA	961	101,409
	10 20 30 40 50 60 MLQTTLLALQ GLSCMNCAQR VKAALESRED VHHAEVNVHY AKVTGEADTH ALIETIKQTG 70 80 90 100 110 120 YQATEAQTPD VELHLSGLSC GHCTETVRKA LEAVSGVISA DVTLESANVY GKADIQTLIA 130 140 150 160 170 180 AVEQAGYHAT QQGIDSPKTE PLTHSAQSQP ESLAAAPNTV PATNVALATS TVSDTNTVLP 190 200 210 220 230 240 TNTALPTNTT STTSTADTAS ATSTAPVINP LPVTESVAQP AASEGESVQL LLTGMSCASC 250 260 270 280 290 300 VSKVQNALQR VDGVQVARVN LAERSALVTG TQNNEALIAA VKNAGYGAEI IEDEGERRER 310 320 330 340 350 360 QQQMSQASMK RFQWQAALGL LLGIPLMAWG LFGGSMTLTP ETQTPWLIIG IITLLVMIFA 370 380 390 400 410 420 GGHFYRNAWV SLKNGRATMD TLVALGTGAA WIYSITVNIW PDVFPMEARH LYYEASAMII 430 440 450 460 470 480 GLINLGHAME QRARQRSSNA LERLLDLAPP TAKLVTDDGE KVIPLADVQL GMILRLTTGD 490 500 510 520 530 540 RVPVDGEIVQ GEVWMDEAML TGEPIPQQKS VGDIVHAGTQ VQDGTVQFRA SAIGSQTTLA 550 560 570 580 590 600 RIIKLVRQAQ SSKPEIGKLA DRISAVFVPT VVVIAIVAGL IWYFFGPQPQ LVYTLVVATT 610 620 630 640 650 660 VLIIACPCAL GLATPMSIIS GVGRAAEFGV LVRDADALQQ ASNLDTLVFD KTGTLTEGHP 670 680 690 700 710 720 QVVAIHTFNG VSEQQALGWA AALETGSNHP LARAILQRAE GLTLATASQF RTLRGLGVSG 730 740 750 760 770 780 EVDGIPLLLG NNRLLEEQQI DTRELQSLIQ QQAESGATPV ILTANGKPAA LLSIRDPLRE 790 800 810 820 830 840 DSIGALQRLH QLGYSLVMLT GDNPITANAI AKEAGIDRVI AGVLPDGKAD AIKQLQAAGH 850 860 870 880 890 900 KVAMIGDGIN DAPALAQADV GIAMGGGSDI AIETAAITLM RHSLYGVVDA VELSKATLRN 910 920 930 940 950 960 MKQNLLGAFF YNALGIPIAA GILYPFTGTL LSPVVAGAAM ALSSITVVSN ANRLLRFKPK Q

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL590842 Genomic DNA. Translation: CAL21688.1. AE009952 Genomic DNA. Translation: AAM84670.1. AE017042 Genomic DNA. Translation: AAS61097.1.
PIR	AE0375.
RefSeq	NP_668419.1. NP_992220.1.
3D structure databases
HSSP	HSSP built from PDB template 1AFJ based on UniProtKB P04129.
ModBase	Search...
Genome annotation databases
GeneID	1146039. 2764600.
GenomeReviews	Gene locus y1093 in contig AE009952_GR. Gene locus YP_0838 in contig AE017042_GR. Gene locus YPO3086 in contig AL590842_GR.
KEGG	ype:YPO3086. ypk:y1093. ypm:YP_0838.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ZCA7.
Enzyme and pathway databases
BioCyc	YPES187410:Y1093-MON. YPES214092:YPO3086-MON. YPES229193:YP0838-MON.
Family and domain databases
InterPro	IPR006416. ATPase-IB_hvy. IPR001757. ATPase_P. IPR006403. ATPase_P_cat/Cu. IPR005834. Dehalogen-like_hydro. IPR008250. E1-E2_ATPase_reg. IPR000695. H_ATPase. IPR006121. HeavyMe_transpt. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 3 hits. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 3 hits. PS50846. HMA_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COPA_YERPE

Accession

Primary (citable) accession number: Q8ZCA7
Secondary accession number(s): Q0WCI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	March 1, 2002
Last modified:	November 25, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)