Reviewed, UniProtKB/Swiss-Prot Q8ZC51 (PANE_YERPE)

Names and origin

Protein names

Recommended name:
    2-dehydropantoate 2-reductase
    EC=1.1.1.169
Alternative name(s):
    Ketopantoate reductase
      Short name=KPA reductase
      Short name=KPR

Gene names

Name:	panE
Synonyms:	apbA
Ordered Locus Names:	YPO3171, y1014, YP_0760

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	2-dehydropantoate 2-reductase		PRO_0000157307
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	176	1	Proton donor By similarity
	Binding site	31	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	122	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	256	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZC51-1 [UniParc]. Last modified February 21, 2002. Version 1. Checksum: 3B1D8198D053B9BE	FASTA	303	33,844
	10 20 30 40 50 60 MKITVLGCGA LGQLWLSMLH QQDHDVQGWL RVPQPFCSVN VIMLNGESFN RNLTTNDPKH 70 80 90 100 110 120 LLQSELLLVC LKAWQVSSAV TALLPKLNPE CKILLLHNGM GTQEELPLNE HVFLHGVTTH 130 140 150 160 170 180 AARRDGNTII HVASGMTHIG PTSSVIIDDN HLADTLHQAL PDVAWHNDIS AACWQKLAVN 190 200 210 220 230 240 CVINPLTGLY NCRNGDVQRY PELIERLCAE VASVMEMEGY HTSTESLLSY VNNVIRSTAD 250 260 270 280 290 300 NTSSLLQDLR SQRHTEIDYI TGYLLRRARS HGMALPENAR LYELIKRKES DYERIGAGLP GSW

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL21766.1. AE009952 Genomic DNA. Translation: AAM84595.1. AE017042 Genomic DNA. Translation: AAS61025.1.
PIR	AC0385.
RefSeq	NP_668344.1. NP_992148.1. YP_002348076.1.
3D structure databases
SMR	Q8ZC51. Positions 1-293.
ModBase	Search...
Genome annotation databases
GeneID	1145961. 1175986. 2764026.
GenomeReviews	Gene locus y1014 in contig AE009952_GR. Gene locus YP_0760 in contig AE017042_GR. Gene locus YPO3171 in contig AL590842_GR.
KEGG	ype:YPO3171. ypk:y1014. ypm:YP_0760.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668370.
OMA	QCHILLL.
Enzyme and pathway databases
BioCyc	YPES187410:Y1014-MONOMER. YPES214092:YPO3171-MONOMER. YPES229193:YP0760-MONOMER.
BRENDA	1.1.1.169. 142588.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21708:SF21. ApbA. 1 hit.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_YERPE

Accession

Primary (citable) accession number: Q8ZC51
Secondary accession number(s): Q0WCB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)