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Protein

Glutamate 5-kinase

Gene

proB

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB), Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATPUniRule annotation1
Binding sitei50SubstrateUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Binding sitei149Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 170ATPUniRule annotation2
Nucleotide bindingi211 – 217ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:YPO3222, y0966, YP_0711
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097621 – 367Glutamate 5-kinaseAdd BLAST367

Proteomic databases

PRIDEiQ8ZC08.

Interactioni

Protein-protein interaction databases

IntActiQ8ZC08. 1 interactor.
STRINGi187410.y0966.

Structurei

3D structure databases

ProteinModelPortaliQ8ZC08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini275 – 353PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OMAiVIHRDHW.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZC08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSQTLVVK LGTSVLTGGS RRLNRAHIVE LVRQCAQQHA KGHRIVIVTS
60 70 80 90 100
GAIAAGREHL GYPELPATIA SKQLLAAVGQ SRLIQLWEQL FSIYGIHIGQ
110 120 130 140 150
MLLTRADLED RERFLNARDT MNALLDNRIV PVINENDAVA TAEIKVGDND
160 170 180 190 200
NLSALAAILA SADKLLLLTD QAGLYTADPR NNPEAELIRE VHGIDDVLRG
210 220 230 240 250
MAGDSVSGLG TGGMATKLQA ADVACRAGID VVIAAGSQVG VIADVIDGTP
260 270 280 290 300
VGTRFHSLET PLENRKRWIF GAPPAGEITV DDGAVFAIME RGSSLLPKGI
310 320 330 340 350
RSVKGDFSRG EVIRIRNLNG RDLAHGVSRY NSDALRMLAG HHSQQISEIL
360
GYEYGPVAVH RDDMIVS
Length:367
Mass (Da):39,291
Last modified:March 1, 2002 - v1
Checksum:i2B0760BA11BF5E05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21816.1.
AE009952 Genomic DNA. Translation: AAM84547.1.
AE017042 Genomic DNA. Translation: AAS60977.1.
PIRiAE0391.
RefSeqiWP_002208701.1. NZ_LQBA01000007.1.
YP_002348124.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84547; AAM84547; y0966.
AAS60977; AAS60977; YP_0711.
GeneIDi1176036.
KEGGiype:YPO3222.
ypk:y0966.
ypm:YP_0711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21816.1.
AE009952 Genomic DNA. Translation: AAM84547.1.
AE017042 Genomic DNA. Translation: AAS60977.1.
PIRiAE0391.
RefSeqiWP_002208701.1. NZ_LQBA01000007.1.
YP_002348124.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ8ZC08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8ZC08. 1 interactor.
STRINGi187410.y0966.

Proteomic databases

PRIDEiQ8ZC08.

Protocols and materials databases

DNASUi1145913.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM84547; AAM84547; y0966.
AAS60977; AAS60977; YP_0711.
GeneIDi1176036.
KEGGiype:YPO3222.
ypk:y0966.
ypm:YP_0711.

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OMAiVIHRDHW.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_YERPE
AccessioniPrimary (citable) accession number: Q8ZC08
Secondary accession number(s): Q0WC64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.