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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi75 – 751Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi110 – 1101Iron-sulfur (2Fe-2S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3188-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Ordered Locus Names:YPO3235, y0956, YP_0698
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Na(+)-translocating NADH-quinone reductase subunit FPRO_0000074512Add
BLAST

Proteomic databases

PRIDEiQ8ZBZ5.

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y0956.

Structurei

3D structure databases

ProteinModelPortaliQ8ZBZ5.
SMRiQ8ZBZ5. Positions 128-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 126952Fe-2S ferredoxin-typeUniRule annotationAdd
BLAST
Domaini129 – 269141FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotation
Contains 1 2Fe-2S ferredoxin-type domain.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
HOGENOMiHOG000263661.
KOiK00351.
OMAiEEHGIIM.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
PRINTSiPR00371. FPNCR.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZBZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIILGVVMF TLIVLALTVM ILFAKSKLVN TGDITVEINE DEDKSFTAPA
60 70 80 90 100
GDKLLNMLSS HGIFVSSACG GGGSCGQCRV TIKEGGGDIL PTELSHISKR
110 120 130 140 150
EAKEGCRLAC QVNVKQNLKI ELPEEIFGVK KWTCEVISND NKATFIKELK
160 170 180 190 200
LKIPDGDVVP FRAGGFIQIE AEPHTVKYAD FDVPTEYRGD WDKFNLFRFE
210 220 230 240 250
SVVTEPTVRA YSMANYPEEH GIILLNVRIA TPPPSVPDAP PGIMSSYIWS
260 270 280 290 300
LKPGDKVVIS GPFGEFFAKD TDAEMVFIGG GAGMAPMRSH IFDQLKRLHS
310 320 330 340 350
KRKISFWYGA RSRREMFYEE DFDQLQAEND NFRWHVALSD PQPEDNWTGY
360 370 380 390 400
TGFIHNVLLE NYLKDHPAPE DCEFYMCGPP MMNAAVIKML KDLGVEDENI

MLDDFGG
Length:407
Mass (Da):45,455
Last modified:March 1, 2002 - v1
Checksum:i24AEFDA035483136
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21829.1.
AE009952 Genomic DNA. Translation: AAM84537.1.
AE017042 Genomic DNA. Translation: AAS60964.1.
PIRiAB0393.
RefSeqiWP_002208711.1. NZ_LQAY01000051.1.
YP_002348137.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84537; AAM84537; y0956.
AAS60964; AAS60964; YP_0698.
GeneIDi1176049.
KEGGiype:YPO3235.
ypj:CH55_1808.
ypk:y0956.
ypl:CH46_1872.
ypm:YP_0698.
ypv:BZ15_299.
ypw:CH59_2835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21829.1.
AE009952 Genomic DNA. Translation: AAM84537.1.
AE017042 Genomic DNA. Translation: AAS60964.1.
PIRiAB0393.
RefSeqiWP_002208711.1. NZ_LQAY01000051.1.
YP_002348137.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ8ZBZ5.
SMRiQ8ZBZ5. Positions 128-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y0956.

Proteomic databases

PRIDEiQ8ZBZ5.

Protocols and materials databases

DNASUi1145903.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM84537; AAM84537; y0956.
AAS60964; AAS60964; YP_0698.
GeneIDi1176049.
KEGGiype:YPO3235.
ypj:CH55_1808.
ypk:y0956.
ypl:CH46_1872.
ypm:YP_0698.
ypv:BZ15_299.
ypw:CH59_2835.

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
HOGENOMiHOG000263661.
KOiK00351.
OMAiEEHGIIM.

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3188-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
PRINTSiPR00371. FPNCR.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQRF_YERPE
AccessioniPrimary (citable) accession number: Q8ZBZ5
Secondary accession number(s): Q0WC51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.