Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi75Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi78Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi110Iron-sulfur (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIon transport, Sodium transport, Transport
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Ordered Locus Names:YPO3235, y0956, YP_0698
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745121 – 407Na(+)-translocating NADH-quinone reductase subunit FAdd BLAST407

Proteomic databases

PaxDbiQ8ZBZ5
PRIDEiQ8ZBZ5

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y0956

Structurei

3D structure databases

ProteinModelPortaliQ8ZBZ5
SMRiQ8ZBZ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 1262Fe-2S ferredoxin-typeUniRule annotationAdd BLAST95
Domaini129 – 269FAD-binding FR-typeUniRule annotationAdd BLAST141

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D26 Bacteria
COG2871 LUCA
HOGENOMiHOG000263661
KOiK00351
OMAiYWYGGRS

Family and domain databases

Gene3Di3.10.20.30, 1 hit
HAMAPiMF_00430 NqrF, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR012675 Beta-grasp_dom_sf
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR010205 NqrF
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000044 Cis_Diol_DH_RD, 1 hit
PRINTSiPR00371 FPNCR
SUPFAMiSSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR01941 nqrF, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZBZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIILGVVMF TLIVLALTVM ILFAKSKLVN TGDITVEINE DEDKSFTAPA
60 70 80 90 100
GDKLLNMLSS HGIFVSSACG GGGSCGQCRV TIKEGGGDIL PTELSHISKR
110 120 130 140 150
EAKEGCRLAC QVNVKQNLKI ELPEEIFGVK KWTCEVISND NKATFIKELK
160 170 180 190 200
LKIPDGDVVP FRAGGFIQIE AEPHTVKYAD FDVPTEYRGD WDKFNLFRFE
210 220 230 240 250
SVVTEPTVRA YSMANYPEEH GIILLNVRIA TPPPSVPDAP PGIMSSYIWS
260 270 280 290 300
LKPGDKVVIS GPFGEFFAKD TDAEMVFIGG GAGMAPMRSH IFDQLKRLHS
310 320 330 340 350
KRKISFWYGA RSRREMFYEE DFDQLQAEND NFRWHVALSD PQPEDNWTGY
360 370 380 390 400
TGFIHNVLLE NYLKDHPAPE DCEFYMCGPP MMNAAVIKML KDLGVEDENI

MLDDFGG
Length:407
Mass (Da):45,455
Last modified:March 1, 2002 - v1
Checksum:i24AEFDA035483136
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL21829.1
AE009952 Genomic DNA Translation: AAM84537.1
AE017042 Genomic DNA Translation: AAS60964.1
PIRiAB0393
RefSeqiWP_002208711.1, NZ_PDBR01000012.1
YP_002348137.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM84537; AAM84537; y0956
AAS60964; AAS60964; YP_0698
GeneIDi1176049
KEGGiype:YPO3235
ypk:y0956
ypm:YP_0698
PATRICifig|214092.21.peg.3695

Similar proteinsi

Entry informationi

Entry nameiNQRF_YERPE
AccessioniPrimary (citable) accession number: Q8ZBZ5
Secondary accession number(s): Q0WC51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health