Q8ZBU2 (GSH1_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	YPO3301, y0887, YP_0385

Organism

Yersinia pestis

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.
Sequence caution	The sequence AAM84471.1 differs from that shown. Reason: Erroneous initiation. The sequence AAS60658.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Glutamate--cysteine ligase HAMAP MF_00578		PRO_0000192547

Sequences

	Sequence		Length	Mass (Da)
	Q8ZBU2 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 3B639BF12E4286A2	FASTA	519	58,471
	10 20 30 40 50 60 MIPDVSHALT WLEAHPKALK GIRRGIERET LRVTADGHLA STGHPESLGA ALTHQWITTD 70 80 90 100 110 120 FAEALLEFIT PVDGDIDHLL TFLRDIHRYT ARKLGDERMW PLSMPCFIEA EQDIELAKYG 130 140 150 160 170 180 SSNIGRFKTL YREGLKNRYG ALMQTISGVH YNFSLPLEFW QAWAGVTDEQ SGKEEISAGY 190 200 210 220 230 240 FRLIRNYYRF GWVIPYLFGA SPAICSSFLQ GRETALPFER NGKGMCYLPY ATSLRLSDLG 250 260 270 280 290 300 YTNKSQSNLG ITFNDLHTYV DALKRAIQTP SEEYVALGLK DGDRHLQLNT NVLQIENELY 310 320 330 340 350 360 APIRPKRVTR AGESPSDALL RGGIEYIEVR SLDINPFSPI GVDAVQARFL DLFLIWCVLA 370 380 390 400 410 420 DAPEMSSDEL LCTRKNWDRV ILEGRKPGQT IGIGCSDSRQ PLETVGKALF ADLRRVAEVL 430 440 450 460 470 480 DGSESASYQQ VCDELVASFD DPELTFSARI LKVMQEKGSG GVGLELAEHY REMLQNEPLE 490 500 510 LLTEEQLSAE RDASRQRQHE LELKDKLSFE EYLALHGGQ

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL21892.1. AE009952 Genomic DNA. Translation: AAM84471.1. Different initiation. AE017042 Genomic DNA. Translation: AAS60658.1. Different initiation.
PIR	AI0400.
RefSeq	NP_668220.1. NC_004088.1. NP_991781.1. NC_005810.1. YP_002348197.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZBU2.
SMR	Q8ZBU2. Positions 1-502.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1145834. 1176117. 2764400.
GenomeReviews	Gene locus y0887 in contig AE009952_GR. Gene locus YP_0385 in contig AE017042_GR. Gene locus YPO3301 in contig AL590842_GR.
KEGG	ype:YPO3301. ypk:y0887. ypm:YP_0385.
Phylogenomic databases
HOGENOM	HBG289023.
OMA	FGLIRNY.
ProtClustDB	PRK02107.
Enzyme and pathway databases
BioCyc	YPES187410:Y0887-MONOMER. YPES214092:YPO3301-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_YERPE

Accession

Primary (citable) accession number: Q8ZBU2
Secondary accession number(s): Q0WBZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program