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Protein

Glutamate-1-semialdehyde 2,1-aminomutase

Gene

hemL

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (hemA), Glutamyl-tRNA reductase (hemA)
  2. Glutamate-1-semialdehyde 2,1-aminomutase (hemL), Glutamate-1-semialdehyde 2,1-aminomutase (hemL)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00317.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutaseUniRule annotation (EC:5.4.3.8UniRule annotation)
Short name:
GSAUniRule annotation
Alternative name(s):
Glutamate-1-semialdehyde aminotransferaseUniRule annotation
Short name:
GSA-ATUniRule annotation
Gene namesi
Name:hemLUniRule annotation
Ordered Locus Names:YPO3389, y0799, YP_0296
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001204741 – 426Glutamate-1-semialdehyde 2,1-aminomutaseAdd BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265N6-(pyridoxal phosphate)lysineUniRule annotation1

Proteomic databases

PRIDEiQ8ZBL9.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8ZBL9. 1 interactor.
STRINGi187410.y0799.

Structurei

Secondary structure

1426
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Helixi16 – 18Combined sources3
Helixi22 – 25Combined sources4
Turni26 – 29Combined sources4
Beta strandi36 – 41Combined sources6
Beta strandi43 – 46Combined sources4
Beta strandi51 – 56Combined sources6
Helixi57 – 59Combined sources3
Helixi69 – 79Combined sources11
Helixi90 – 102Combined sources13
Beta strandi107 – 114Combined sources8
Helixi115 – 130Combined sources16
Beta strandi134 – 138Combined sources5
Helixi170 – 173Combined sources4
Beta strandi176 – 179Combined sources4
Helixi184 – 193Combined sources10
Turni195 – 197Combined sources3
Beta strandi198 – 203Combined sources6
Beta strandi205 – 207Combined sources3
Helixi219 – 230Combined sources12
Beta strandi233 – 238Combined sources6
Turni239 – 244Combined sources6
Helixi250 – 253Combined sources4
Beta strandi259 – 264Combined sources6
Helixi265 – 268Combined sources4
Beta strandi274 – 278Combined sources5
Helixi280 – 283Combined sources4
Turni287 – 289Combined sources3
Beta strandi290 – 292Combined sources3
Helixi302 – 315Combined sources14
Helixi320 – 341Combined sources22
Beta strandi347 – 351Combined sources5
Beta strandi354 – 358Combined sources5
Beta strandi361 – 363Combined sources3
Helixi368 – 372Combined sources5
Helixi376 – 388Combined sources13
Helixi409 – 423Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4E77X-ray2.00A1-426[»]
ProteinModelPortaliQ8ZBL9.
SMRiQ8ZBL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDM. Bacteria.
COG0001. LUCA.
HOGENOMiHOG000020210.
KOiK01845.
OMAiCGHAHPE.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3. 1 hit.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZBL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSENLYAQ AQQLIPGGVN SPVRAFTGVG GIPLFIERAD GAYLFDVDGK
60 70 80 90 100
AYIDYVGSWG PMILGHNHPA IRQAVIEAVE RGLSFGAPTE MEVKMAQLVT
110 120 130 140 150
DLVPTMDMVR MVNSGTEATM SAIRLARGYT GRDKIIKFEG CYHGHADCLL
160 170 180 190 200
VKAGSGALTL GQPNSPGVPT DFAKHTLTCT YNDLASVRQA FEQYPQEVAC
210 220 230 240 250
IIVEPVAGNM NCIPPLPEFL PGLRALCDEF GALLIIDEVM TGFRVALAGA
260 270 280 290 300
QDYYHVIPDL TCLGKIIGGG MPVGAFGGRR EVMNALAPTG PVYQAGTLSG
310 320 330 340 350
NPIAMAAGFA CLTEISQVGV YETLTELTDS LATGLRHAAK EENIPLVVNH
360 370 380 390 400
VGGMFGLFFT NADTVTCYQD VMNCDVERFK RFFHLMLEEG VYLAPSAFEA
410 420
GFMSLAHSNE DIQKTVNAAR RCFAKL
Length:426
Mass (Da):45,794
Last modified:March 1, 2002 - v1
Checksum:i1BB579AE82D49BA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21978.1.
AE009952 Genomic DNA. Translation: AAM84386.1.
AE017042 Genomic DNA. Translation: AAS60571.1.
PIRiAG0411.
RefSeqiWP_002209362.1. NZ_LQBA01000021.1.
YP_002348281.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84386; AAM84386; y0799.
AAS60571; AAS60571; YP_0296.
GeneIDi1176206.
KEGGiype:YPO3389.
ypk:y0799.
ypm:YP_0296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL21978.1.
AE009952 Genomic DNA. Translation: AAM84386.1.
AE017042 Genomic DNA. Translation: AAS60571.1.
PIRiAG0411.
RefSeqiWP_002209362.1. NZ_LQBA01000021.1.
YP_002348281.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4E77X-ray2.00A1-426[»]
ProteinModelPortaliQ8ZBL9.
SMRiQ8ZBL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8ZBL9. 1 interactor.
STRINGi187410.y0799.

Proteomic databases

PRIDEiQ8ZBL9.

Protocols and materials databases

DNASUi1145746.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM84386; AAM84386; y0799.
AAS60571; AAS60571; YP_0296.
GeneIDi1176206.
KEGGiype:YPO3389.
ypk:y0799.
ypm:YP_0296.

Phylogenomic databases

eggNOGiENOG4105CDM. Bacteria.
COG0001. LUCA.
HOGENOMiHOG000020210.
KOiK01845.
OMAiCGHAHPE.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00317.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3. 1 hit.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSA_YERPE
AccessioniPrimary (citable) accession number: Q8ZBL9
Secondary accession number(s): Q0WBQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.