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Protein

3-dehydroquinate dehydratase

Gene

aroQ

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a trans-dehydration via an enolate intermediate.UniRule annotation

Catalytic activityi

3-dehydroquinate = 3-dehydroshikimate + H2O.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 2 (AU253_01310), Shikimate kinase (aroK), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei21Transition state stabilizerUniRule annotation1
Active sitei26Proton acceptorUniRule annotation1
Binding sitei77SubstrateUniRule annotation1
Binding sitei83SubstrateUniRule annotation1
Binding sitei90SubstrateUniRule annotation1
Active sitei103Proton donorUniRule annotation1
Binding sitei114SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00053; UER00086.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate dehydrataseUniRule annotation (EC:4.2.1.10UniRule annotation)
Short name:
3-dehydroquinaseUniRule annotation
Alternative name(s):
Type II DHQaseUniRule annotation
Gene namesi
Name:aroQUniRule annotation
Ordered Locus Names:YPO3660, y0207, YP_3886
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001599461 – 1503-dehydroquinate dehydrataseAdd BLAST150

Interactioni

Subunit structurei

Homododecamer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y0207.

Structurei

Secondary structure

1150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 11Combined sources7
Helixi15 – 17Combined sources3
Turni18 – 20Combined sources3
Helixi23 – 26Combined sources4
Helixi31 – 44Combined sources14
Beta strandi47 – 53Combined sources7
Helixi57 – 67Combined sources11
Turni68 – 70Combined sources3
Beta strandi72 – 77Combined sources6
Helixi79 – 83Combined sources5
Helixi86 – 95Combined sources10
Beta strandi99 – 105Combined sources7
Helixi107 – 109Combined sources3
Helixi112 – 115Combined sources4
Helixi120 – 122Combined sources3
Beta strandi123 – 130Combined sources8
Helixi133 – 148Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LWZX-ray1.65A/B/C/D1-150[»]
ProteinModelPortaliQ8ZAX1.
SMRiQ8ZAX1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZAX1.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 105Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the type-II 3-dehydroquinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Z38. Bacteria.
COG0757. LUCA.
HOGENOMiHOG000217278.
KOiK03786.
OMAiRREPGHY.

Family and domain databases

CDDicd00466. DHQase_II. 1 hit.
Gene3Di3.40.50.9100. 1 hit.
HAMAPiMF_00169. AroQ. 1 hit.
InterProiIPR001874. DHquinase_II.
IPR018509. DHquinase_II_CS.
[Graphical view]
PANTHERiPTHR21272. PTHR21272. 1 hit.
PfamiPF01220. DHquinase_II. 1 hit.
[Graphical view]
PIRSFiPIRSF001399. DHquinase_II. 1 hit.
ProDomiPD004527. DHquinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52304. SSF52304. 1 hit.
TIGRFAMsiTIGR01088. aroQ. 1 hit.
PROSITEiPS01029. DEHYDROQUINASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZAX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKFHILLL NGPNLNLLGT REPEKYGYTT LAEIVSQLEI QAQGMDVALS
60 70 80 90 100
HLQSNAEHAL IDSIHQARGN TDFILINPAA FTHTSVALRD ALLGVQIPFI
110 120 130 140 150
EIHLSNVHAR EPFRHHSYLS DIAVGVICGL GADGYNFALQ AAVNRLSKSN
Length:150
Mass (Da):16,395
Last modified:March 1, 2002 - v1
Checksum:i76D8085848BD78E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22249.1.
AE009952 Genomic DNA. Translation: AAM83801.1.
AE017042 Genomic DNA. Translation: AAS64031.1.
PIRiAF0445.
RefSeqiWP_002210071.1. NZ_LQBA01000077.1.
YP_002348546.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM83801; AAM83801; y0207.
AAS64031; AAS64031; YP_3886.
GeneIDi1176481.
KEGGiype:YPO3660.
ypk:y0207.
ypm:YP_3886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22249.1.
AE009952 Genomic DNA. Translation: AAM83801.1.
AE017042 Genomic DNA. Translation: AAS64031.1.
PIRiAF0445.
RefSeqiWP_002210071.1. NZ_LQBA01000077.1.
YP_002348546.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LWZX-ray1.65A/B/C/D1-150[»]
ProteinModelPortaliQ8ZAX1.
SMRiQ8ZAX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y0207.

Protocols and materials databases

DNASUi1145154.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM83801; AAM83801; y0207.
AAS64031; AAS64031; YP_3886.
GeneIDi1176481.
KEGGiype:YPO3660.
ypk:y0207.
ypm:YP_3886.

Phylogenomic databases

eggNOGiENOG4108Z38. Bacteria.
COG0757. LUCA.
HOGENOMiHOG000217278.
KOiK03786.
OMAiRREPGHY.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00086.

Miscellaneous databases

EvolutionaryTraceiQ8ZAX1.

Family and domain databases

CDDicd00466. DHQase_II. 1 hit.
Gene3Di3.40.50.9100. 1 hit.
HAMAPiMF_00169. AroQ. 1 hit.
InterProiIPR001874. DHquinase_II.
IPR018509. DHquinase_II_CS.
[Graphical view]
PANTHERiPTHR21272. PTHR21272. 1 hit.
PfamiPF01220. DHquinase_II. 1 hit.
[Graphical view]
PIRSFiPIRSF001399. DHquinase_II. 1 hit.
ProDomiPD004527. DHquinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52304. SSF52304. 1 hit.
TIGRFAMsiTIGR01088. aroQ. 1 hit.
PROSITEiPS01029. DEHYDROQUINASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROQ_YERPE
AccessioniPrimary (citable) accession number: Q8ZAX1
Secondary accession number(s): Q0WAZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.