Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-dehydroquinate dehydratase

Gene

aroQ

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a trans-dehydration via an enolate intermediate.UniRule annotation

Catalytic activityi

3-dehydroquinate = 3-dehydroshikimate + H2O.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase 2 (aroL), Shikimate kinase 2 (AU253_01310), Shikimate kinase (aroK), Shikimate kinase 1 (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (AVO31_06505), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei21 – 211Transition state stabilizerUniRule annotation
Active sitei26 – 261Proton acceptorUniRule annotation
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei83 – 831SubstrateUniRule annotation
Binding sitei90 – 901SubstrateUniRule annotation
Active sitei103 – 1031Proton donorUniRule annotation
Binding sitei114 – 1141SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3618-MONOMER.
UniPathwayiUPA00053; UER00086.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate dehydrataseUniRule annotation (EC:4.2.1.10UniRule annotation)
Short name:
3-dehydroquinaseUniRule annotation
Alternative name(s):
Type II DHQaseUniRule annotation
Gene namesi
Name:aroQUniRule annotation
Ordered Locus Names:YPO3660, y0207, YP_3886
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1501503-dehydroquinate dehydratasePRO_0000159946Add
BLAST

Interactioni

Subunit structurei

Homododecamer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y0207.

Structurei

Secondary structure

1
150
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117Combined sources
Helixi15 – 173Combined sources
Turni18 – 203Combined sources
Helixi23 – 264Combined sources
Helixi31 – 4414Combined sources
Beta strandi47 – 537Combined sources
Helixi57 – 6711Combined sources
Turni68 – 703Combined sources
Beta strandi72 – 776Combined sources
Helixi79 – 835Combined sources
Helixi86 – 9510Combined sources
Beta strandi99 – 1057Combined sources
Helixi107 – 1093Combined sources
Helixi112 – 1154Combined sources
Helixi120 – 1223Combined sources
Beta strandi123 – 1308Combined sources
Helixi133 – 14816Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LWZX-ray1.65A/B/C/D1-150[»]
ProteinModelPortaliQ8ZAX1.
SMRiQ8ZAX1. Positions 7-148.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZAX1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1052Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the type-II 3-dehydroquinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Z38. Bacteria.
COG0757. LUCA.
HOGENOMiHOG000217278.
KOiK03786.
OMAiIPTIEVH.
OrthoDBiEOG66MQVT.

Family and domain databases

Gene3Di3.40.50.9100. 1 hit.
HAMAPiMF_00169. AroQ.
InterProiIPR001874. DHquinase_II.
IPR018509. DHquinase_II_CS.
[Graphical view]
PANTHERiPTHR21272. PTHR21272. 1 hit.
PfamiPF01220. DHquinase_II. 1 hit.
[Graphical view]
PIRSFiPIRSF001399. DHquinase_II. 1 hit.
ProDomiPD004527. DHquinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52304. SSF52304. 1 hit.
TIGRFAMsiTIGR01088. aroQ. 1 hit.
PROSITEiPS01029. DEHYDROQUINASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZAX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKFHILLL NGPNLNLLGT REPEKYGYTT LAEIVSQLEI QAQGMDVALS
60 70 80 90 100
HLQSNAEHAL IDSIHQARGN TDFILINPAA FTHTSVALRD ALLGVQIPFI
110 120 130 140 150
EIHLSNVHAR EPFRHHSYLS DIAVGVICGL GADGYNFALQ AAVNRLSKSN
Length:150
Mass (Da):16,395
Last modified:March 1, 2002 - v1
Checksum:i76D8085848BD78E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22249.1.
AE009952 Genomic DNA. Translation: AAM83801.1.
AE017042 Genomic DNA. Translation: AAS64031.1.
PIRiAF0445.
RefSeqiWP_002210071.1. NZ_LQAY01000085.1.
YP_002348546.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM83801; AAM83801; y0207.
AAS64031; AAS64031; YP_3886.
GeneIDi1176481.
KEGGiype:YPO3660.
ypj:CH55_2541.
ypk:y0207.
ypl:CH46_1455.
ypm:YP_3886.
ypv:BZ15_4104.
ypw:CH59_2415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22249.1.
AE009952 Genomic DNA. Translation: AAM83801.1.
AE017042 Genomic DNA. Translation: AAS64031.1.
PIRiAF0445.
RefSeqiWP_002210071.1. NZ_LQAY01000085.1.
YP_002348546.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LWZX-ray1.65A/B/C/D1-150[»]
ProteinModelPortaliQ8ZAX1.
SMRiQ8ZAX1. Positions 7-148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi187410.y0207.

Protocols and materials databases

DNASUi1145154.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM83801; AAM83801; y0207.
AAS64031; AAS64031; YP_3886.
GeneIDi1176481.
KEGGiype:YPO3660.
ypj:CH55_2541.
ypk:y0207.
ypl:CH46_1455.
ypm:YP_3886.
ypv:BZ15_4104.
ypw:CH59_2415.

Phylogenomic databases

eggNOGiENOG4108Z38. Bacteria.
COG0757. LUCA.
HOGENOMiHOG000217278.
KOiK03786.
OMAiIPTIEVH.
OrthoDBiEOG66MQVT.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00086.
BioCyciYPES214092:GKDD-3618-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8ZAX1.

Family and domain databases

Gene3Di3.40.50.9100. 1 hit.
HAMAPiMF_00169. AroQ.
InterProiIPR001874. DHquinase_II.
IPR018509. DHquinase_II_CS.
[Graphical view]
PANTHERiPTHR21272. PTHR21272. 1 hit.
PfamiPF01220. DHquinase_II. 1 hit.
[Graphical view]
PIRSFiPIRSF001399. DHquinase_II. 1 hit.
ProDomiPD004527. DHquinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52304. SSF52304. 1 hit.
TIGRFAMsiTIGR01088. aroQ. 1 hit.
PROSITEiPS01029. DEHYDROQUINASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiAROQ_YERPE
AccessioniPrimary (citable) accession number: Q8ZAX1
Secondary accession number(s): Q0WAZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: March 1, 2002
Last modified: July 6, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.