SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8ZAR2

- PUR2_YERPE

UniProt

Q8ZAR2 - PUR2_YERPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Phosphoribosylamine--glycine ligase
Gene
purD, YPO3729, y0501, YP_3092
Organism
Yersinia pestis
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi286 – 2861Magnesium or manganese By similarity
Metal bindingi288 – 2881Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 19662ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3692-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene namesi
Name:purD
Ordered Locus Names:YPO3729, y0501, YP_3092
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Phosphoribosylamine--glycine ligaseUniRule annotation
PRO_0000151506Add
BLAST

Proteomic databases

PRIDEiQ8ZAR2.

Interactioni

Protein-protein interaction databases

IntActiQ8ZAR2. 6 interactions.
STRINGi214092.YPO3729.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 77
Helixi10 – 1910
Beta strandi25 – 328
Helixi35 – 395
Helixi53 – 6210
Beta strandi65 – 706
Helixi73 – 775
Helixi80 – 867
Beta strandi91 – 933
Helixi96 – 1038
Helixi105 – 11410
Beta strandi122 – 1265
Helixi128 – 13811
Beta strandi140 – 14910
Beta strandi154 – 1585
Helixi161 – 17212
Helixi175 – 1784
Beta strandi184 – 1885
Beta strandi192 – 20413
Beta strandi206 – 2083
Beta strandi212 – 2143
Beta strandi217 – 2193
Turni220 – 2223
Beta strandi223 – 2264
Beta strandi230 – 2356
Helixi241 – 25010
Helixi252 – 26110
Beta strandi267 – 27610
Beta strandi282 – 2876
Helixi289 – 2913
Turni293 – 2953
Helixi296 – 3027
Helixi307 – 3159
Helixi319 – 3213
Beta strandi331 – 3388
Turni339 – 3424
Beta strandi362 – 37110
Beta strandi377 – 3793
Beta strandi381 – 39010
Helixi394 – 40512
Helixi421 – 4244
Turni425 – 4273

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MJFX-ray1.47A1-428[»]
ProteinModelPortaliQ8ZAR2.
SMRiQ8ZAR2. Positions 1-426.

Miscellaneous databases

EvolutionaryTraceiQ8ZAR2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 316208ATP-grasp
Add
BLAST

Sequence similaritiesi

Belongs to the GARS family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033463.
KOiK01945.
OMAiDIHWDGS.
OrthoDBiEOG69SKD1.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZAR2-1 [UniParc]FASTAAdd to Basket

« Hide

MNILIIGNGG REHALGWKAA QSPLADKIYV APGNAGTALE PTLENVDIAA    50
TDIAGLLAFA QSHDIGLTIV GPEAPLVIGV VDAFRAAGLA IFGPTQAAAQ 100
LEGSKAFTKD FLARHNIPSA EYQNFTDVEA ALAYVRQKGA PIVIKADGLA 150
AGKGVIVAMT QEEAETAVND MLAGNAFGDA GHRIVVEEFL DGEEASFIVM 200
VDGENVLPMA TSQDHKRVGD GDTGPNTGGM GAYSPAPVVT DDVHQRVMDQ 250
VIWPTVRGMA AEGNIYTGFL YAGLMISADG QPKVIEFNCR FGDPETQPIM 300
LRMRSDLVEL CLAGTQGKLN EKTSDWDERP SLGVVLAAGG YPADYRQGDV 350
IHGLPQQEVK DGKVFHAGTK LNGNHEVVTN GGRVLCVTAL GETVAQAQQY 400
AYQLAEGIQW EGVFCRKDIG YRAIARGK 428
Length:428
Mass (Da):45,317
Last modified:March 27, 2002 - v1
Checksum:i0D12863DD2424EFF
GO

Sequence cautioni

The sequence AAM84090.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAS63262.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL22316.1.
AE009952 Genomic DNA. Translation: AAM84090.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63262.1. Different initiation.
PIRiAI0453.
RefSeqiNP_667839.1. NC_004088.1.
NP_994385.1. NC_005810.1.
YP_002348609.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84090; AAM84090; y0501.
AAS63262; AAS63262; YP_3092.
GeneIDi1145448.
1176555.
2765674.
KEGGiype:YPO3729.
ypk:y0501.
ypm:YP_3092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590842 Genomic DNA. Translation: CAL22316.1 .
AE009952 Genomic DNA. Translation: AAM84090.1 . Different initiation.
AE017042 Genomic DNA. Translation: AAS63262.1 . Different initiation.
PIRi AI0453.
RefSeqi NP_667839.1. NC_004088.1.
NP_994385.1. NC_005810.1.
YP_002348609.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MJF X-ray 1.47 A 1-428 [» ]
ProteinModelPortali Q8ZAR2.
SMRi Q8ZAR2. Positions 1-426.
ModBasei Search...

Protein-protein interaction databases

IntActi Q8ZAR2. 6 interactions.
STRINGi 214092.YPO3729.

Proteomic databases

PRIDEi Q8ZAR2.

Protocols and materials databases

DNASUi 1145448.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM84090 ; AAM84090 ; y0501 .
AAS63262 ; AAS63262 ; YP_3092 .
GeneIDi 1145448.
1176555.
2765674.
KEGGi ype:YPO3729.
ypk:y0501.
ypm:YP_3092.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033463.
KOi K01945.
OMAi DIHWDGS.
OrthoDBi EOG69SKD1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci YPES214092:GKDD-3692-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q8ZAR2.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiPUR2_YERPE
AccessioniPrimary (citable) accession number: Q8ZAR2
Secondary accession number(s): Q0WAS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi