Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8ZAR2

- PUR2_YERPE

UniProt

Q8ZAR2 - PUR2_YERPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Yersinia pestis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn(2+) ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi286 – 2861Magnesium or manganeseUniRule annotation
Metal bindingi288 – 2881Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 19662ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3692-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:YPO3729, y0501, YP_3092
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815: Chromosome, UP000001019: Chromosome, UP000002490: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Phosphoribosylamine--glycine ligasePRO_0000151506Add
BLAST

Proteomic databases

PRIDEiQ8ZAR2.

Interactioni

Protein-protein interaction databases

IntActiQ8ZAR2. 6 interactions.
STRINGi214092.YPO3729.

Structurei

Secondary structure

428
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 77Combined sources
Helixi10 – 1910Combined sources
Beta strandi25 – 328Combined sources
Helixi35 – 395Combined sources
Helixi53 – 6210Combined sources
Beta strandi65 – 706Combined sources
Helixi73 – 775Combined sources
Helixi80 – 867Combined sources
Beta strandi91 – 933Combined sources
Helixi96 – 1038Combined sources
Helixi105 – 11410Combined sources
Beta strandi122 – 1265Combined sources
Helixi128 – 13811Combined sources
Beta strandi140 – 14910Combined sources
Beta strandi154 – 1585Combined sources
Helixi161 – 17212Combined sources
Helixi175 – 1784Combined sources
Beta strandi184 – 1885Combined sources
Beta strandi192 – 20413Combined sources
Beta strandi206 – 2083Combined sources
Beta strandi212 – 2143Combined sources
Beta strandi217 – 2193Combined sources
Turni220 – 2223Combined sources
Beta strandi223 – 2264Combined sources
Beta strandi230 – 2356Combined sources
Helixi241 – 25010Combined sources
Helixi252 – 26110Combined sources
Beta strandi267 – 27610Combined sources
Beta strandi282 – 2876Combined sources
Helixi289 – 2913Combined sources
Turni293 – 2953Combined sources
Helixi296 – 3027Combined sources
Helixi307 – 3159Combined sources
Helixi319 – 3213Combined sources
Beta strandi331 – 3388Combined sources
Turni339 – 3424Combined sources
Beta strandi362 – 37110Combined sources
Beta strandi377 – 3793Combined sources
Beta strandi381 – 39010Combined sources
Helixi394 – 40512Combined sources
Helixi421 – 4244Combined sources
Turni425 – 4273Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MJFX-ray1.47A1-428[»]
ProteinModelPortaliQ8ZAR2.
SMRiQ8ZAR2. Positions 1-426.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8ZAR2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini109 – 316208ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033463.
KOiK01945.
OMAiDIHWDGS.
OrthoDBiEOG69SKD1.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ZAR2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNILIIGNGG REHALGWKAA QSPLADKIYV APGNAGTALE PTLENVDIAA
60 70 80 90 100
TDIAGLLAFA QSHDIGLTIV GPEAPLVIGV VDAFRAAGLA IFGPTQAAAQ
110 120 130 140 150
LEGSKAFTKD FLARHNIPSA EYQNFTDVEA ALAYVRQKGA PIVIKADGLA
160 170 180 190 200
AGKGVIVAMT QEEAETAVND MLAGNAFGDA GHRIVVEEFL DGEEASFIVM
210 220 230 240 250
VDGENVLPMA TSQDHKRVGD GDTGPNTGGM GAYSPAPVVT DDVHQRVMDQ
260 270 280 290 300
VIWPTVRGMA AEGNIYTGFL YAGLMISADG QPKVIEFNCR FGDPETQPIM
310 320 330 340 350
LRMRSDLVEL CLAGTQGKLN EKTSDWDERP SLGVVLAAGG YPADYRQGDV
360 370 380 390 400
IHGLPQQEVK DGKVFHAGTK LNGNHEVVTN GGRVLCVTAL GETVAQAQQY
410 420
AYQLAEGIQW EGVFCRKDIG YRAIARGK
Length:428
Mass (Da):45,317
Last modified:March 27, 2002 - v1
Checksum:i0D12863DD2424EFF
GO

Sequence cautioni

The sequence AAM84090.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAS63262.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22316.1.
AE009952 Genomic DNA. Translation: AAM84090.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63262.1. Different initiation.
PIRiAI0453.
RefSeqiNP_667839.1. NC_004088.1.
NP_994385.1. NC_005810.1.
YP_002348609.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM84090; AAM84090; y0501.
AAS63262; AAS63262; YP_3092.
GeneIDi1145448.
1176555.
2765674.
KEGGiype:YPO3729.
ypk:y0501.
ypm:YP_3092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22316.1 .
AE009952 Genomic DNA. Translation: AAM84090.1 . Different initiation.
AE017042 Genomic DNA. Translation: AAS63262.1 . Different initiation.
PIRi AI0453.
RefSeqi NP_667839.1. NC_004088.1.
NP_994385.1. NC_005810.1.
YP_002348609.1. NC_003143.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3MJF X-ray 1.47 A 1-428 [» ]
ProteinModelPortali Q8ZAR2.
SMRi Q8ZAR2. Positions 1-426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8ZAR2. 6 interactions.
STRINGi 214092.YPO3729.

Proteomic databases

PRIDEi Q8ZAR2.

Protocols and materials databases

DNASUi 1145448.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM84090 ; AAM84090 ; y0501 .
AAS63262 ; AAS63262 ; YP_3092 .
GeneIDi 1145448.
1176555.
2765674.
KEGGi ype:YPO3729.
ypk:y0501.
ypm:YP_3092.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033463.
KOi K01945.
OMAi DIHWDGS.
OrthoDBi EOG69SKD1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci YPES214092:GKDD-3692-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q8ZAR2.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiPUR2_YERPE
AccessioniPrimary (citable) accession number: Q8ZAR2
Secondary accession number(s): Q0WAS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3