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Q8ZAR2 (PUR2_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:YPO3729, y0501, YP_3092
OrganismYersinia pestis [Reference proteome] [HAMAP]
Taxonomic identifier632 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAM84090.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAS63262.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151506

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Secondary structure

............................................................................... 428
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8ZAR2 [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: 0D12863DD2424EFF

FASTA42845,317
        10         20         30         40         50         60 
MNILIIGNGG REHALGWKAA QSPLADKIYV APGNAGTALE PTLENVDIAA TDIAGLLAFA 

        70         80         90        100        110        120 
QSHDIGLTIV GPEAPLVIGV VDAFRAAGLA IFGPTQAAAQ LEGSKAFTKD FLARHNIPSA 

       130        140        150        160        170        180 
EYQNFTDVEA ALAYVRQKGA PIVIKADGLA AGKGVIVAMT QEEAETAVND MLAGNAFGDA 

       190        200        210        220        230        240 
GHRIVVEEFL DGEEASFIVM VDGENVLPMA TSQDHKRVGD GDTGPNTGGM GAYSPAPVVT 

       250        260        270        280        290        300 
DDVHQRVMDQ VIWPTVRGMA AEGNIYTGFL YAGLMISADG QPKVIEFNCR FGDPETQPIM 

       310        320        330        340        350        360 
LRMRSDLVEL CLAGTQGKLN EKTSDWDERP SLGVVLAAGG YPADYRQGDV IHGLPQQEVK 

       370        380        390        400        410        420 
DGKVFHAGTK LNGNHEVVTN GGRVLCVTAL GETVAQAQQY AYQLAEGIQW EGVFCRKDIG 


YRAIARGK 

« Hide

References

[1]"Genome sequence of Yersinia pestis, the causative agent of plague."
Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. expand/collapse author list , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.
Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CO-92 / Biovar Orientalis.
[2]"Genome sequence of Yersinia pestis KIM."
Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. expand/collapse author list , Matson J.S., Blattner F.R., Perry R.D.
J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KIM10+ / Biovar Mediaevalis.
[3]"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. expand/collapse author list , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590842 Genomic DNA. Translation: CAL22316.1.
AE009952 Genomic DNA. Translation: AAM84090.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63262.1. Different initiation.
PIRAI0453.
RefSeqNP_667839.1. NC_004088.1.
NP_994385.1. NC_005810.1.
YP_002348609.1. NC_003143.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3MJFX-ray1.47A1-428[»]
ProteinModelPortalQ8ZAR2.
SMRQ8ZAR2. Positions 1-426.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8ZAR2. 6 interactions.
STRING214092.YPO3729.

Proteomic databases

PRIDEQ8ZAR2.

Protocols and materials databases

DNASU1145448.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM84090; AAM84090; y0501.
AAS63262; AAS63262; YP_3092.
GeneID1145448.
1176555.
2765674.
KEGGype:YPO3729.
ypk:y0501.
ypm:YP_3092.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADIHWDGS.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycYPES214092:GKDD-3692-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8ZAR2.

Entry information

Entry namePUR2_YERPE
AccessionPrimary (citable) accession number: Q8ZAR2
Secondary accession number(s): Q0WAS9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways