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Protein

Thiazole synthase

Gene

thiG

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Schiff-base intermediate with DXPUniRule annotation1
Binding sitei156DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:YPO3742, y0488, YP_3106
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001628821 – 271Thiazole synthaseAdd BLAST271

Proteomic databases

PaxDbiQ8ZAP9

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y0488

Structurei

3D structure databases

ProteinModelPortaliQ8ZAP9
SMRiQ8ZAP9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 183DXP bindingUniRule annotation2
Regioni204 – 205DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ZAP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIADTTFT SRLFTGTGKF SSPELMLEAL RASGSQLITM AMKRVDLQSG
60 70 80 90 100
NDAILAPLRQ LGVRLLPNTS GAKTAEEAIF AARLAREALN THWVKLEVHP
110 120 130 140 150
DVRYLLPDPI ETLKAAEVLV KEGFVVLPYC GADPVLCKRL EEVGCAAVMP
160 170 180 190 200
LGSPIGSNLG LRTRDFLQII IEQSKVPVVV DAGIGAPSHA LEALELGADA
210 220 230 240 250
VLVNTAIAVA HSPVQMAHAF RLAVESGERA RLAGLGASPF NPSQPDTLQL
260 270
RATATSPLTG FLSQLEEQDH V
Length:271
Mass (Da):28,873
Last modified:June 16, 2003 - v2
Checksum:iFB1C6A47331B09CF
GO

Sequence cautioni

The sequence AAM84077 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS63276 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAL22329 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti98V → I (PubMed:12142430).Curated1
Sequence conflicti98V → I (PubMed:15368893).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA Translation: CAL22329.1 Different initiation.
AE009952 Genomic DNA Translation: AAM84077.1 Different initiation.
AE017042 Genomic DNA Translation: AAS63276.1 Different initiation.
PIRiAF0455
RefSeqiWP_002217273.1, NZ_LIXY01000015.1

Genome annotation databases

EnsemblBacteriaiAAM84077; AAM84077; y0488
AAS63276; AAS63276; YP_3106
KEGGiypk:y0488
ypl:CH46_1334
ypm:YP_3106
ypv:BZ15_3983
ypw:CH59_1666
PATRICifig|632.151.peg.2476

Similar proteinsi

Entry informationi

Entry nameiTHIG_YERPE
AccessioniPrimary (citable) accession number: Q8ZAP9
Secondary accession number(s): Q0WAR6, Q8D1H1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health