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Q8ZAL3 (METE_YERPE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

EC=2.1.1.14
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene names
Name:metE
Ordered Locus Names:YPO3788, y0442, YP_3261
OrganismYersinia pestis [Reference proteome] [HAMAP]
Taxonomic identifier632 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP-Rule MF_00172

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP-Rule MF_00172

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP-Rule MF_00172

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family.

Sequence caution

The sequence AAM84031.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7587585-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP-Rule MF_00172
PRO_0000098682

Sites

Metal binding6441Zinc By similarity
Metal binding6461Zinc By similarity
Metal binding7291Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ZAL3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 48F4B4D74BB63C3C

FASTA75885,677
        10         20         30         40         50         60 
MTILNHTLGF PRVGLKRELK KAQESYWAGN STQEELLNVG RELRARHWQQ QQQAGVDLVP 

        70         80         90        100        110        120 
VGDFAWYDHV LTTSLLLGNV PERHQNADGS IDIDTLFRIG RGRAPTGKPA AAAEMTKWFN 

       130        140        150        160        170        180 
TNYHYMVPEF QQGQQFKLGW TQLLDEVDEA LALGHKIKPV LLGPITYLWL GKVKGEQFDR 

       190        200        210        220        230        240 
LSLLNDILPV YQQVLAELAK RGIEWVQIDE PALVLELPQE WLDAYQPAYQ ALQGQVKLLL 

       250        260        270        280        290        300 
TTYFDSIGHN IDTIRALPVQ GLHVDVVTGH DDLAVLNKNL PKEWLLSLGV INGRNVWRAD 

       310        320        330        340        350        360 
LSSWFERLQP LVNSRPLWLG SSCSLLHSPI DLNEETRLDA EVKSWFAFAL QKCAELALLT 

       370        380        390        400        410        420 
QALNAPNDAK LAELAAYSAP IRARRSSSRV HNAQVEQRLA AITSQDIERQ LPYEARAETQ 

       430        440        450        460        470        480 
RKRFNLPAWP TTTIGSFPQT TEIRGLRLDF KQGRLDGKNY RTGISEHIKH AIAEQERLGL 

       490        500        510        520        530        540 
DVLVHGEAER NDMVEYFGEH LDGFVFTQNG WVQSYGSRCV KPPVIIGDIS RPEAITVEWA 

       550        560        570        580        590        600 
KYAQSLTEKP VKGMLTGPVT ILCWSFPRED VSRETIAKQI ALALRDEVED LEKAGIGIIQ 

       610        620        630        640        650        660 
IDEPALREGL PLRRADWQAY LQWAVDAFKL NAAVAQNDTQ IHTHMCYCEF NDIMDSIAAL 

       670        680        690        700        710        720 
DADVITIETS RSDMELLESF EDFAYPNEIG PGVYDIHSPN VPSVEWIEAL LRKAAQRIPA 

       730        740        750 
ERLWVNPDCG LKTRGWPETR QALANMVLAA QRLREEQV 

« Hide

References

[1]"Genome sequence of Yersinia pestis, the causative agent of plague."
Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. expand/collapse author list , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G.
Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CO-92 / Biovar Orientalis.
[2]"Genome sequence of Yersinia pestis KIM."
Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. expand/collapse author list , Matson J.S., Blattner F.R., Perry R.D.
J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KIM10+ / Biovar Mediaevalis.
[3]"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. expand/collapse author list , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590842 Genomic DNA. Translation: CAL22374.1.
AE009952 Genomic DNA. Translation: AAM84031.1. Different initiation.
AE017042 Genomic DNA. Translation: AAS63429.1.
PIRAC0461.
RefSeqNP_667780.1. NC_004088.1.
NP_994552.1. NC_005810.1.
YP_002348665.1. NC_003143.1.

3D structure databases

ProteinModelPortalQ8ZAL3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8ZAL3. 4 interactions.
STRING214092.YPO3788.

Proteomic databases

PRIDEQ8ZAL3.

Protocols and materials databases

DNASU1145389.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM84031; AAM84031; y0442.
AAS63429; AAS63429; YP_3261.
GeneID1145389.
1176622.
2763568.
KEGGype:YPO3788.
ypk:y0442.
ypm:YP_3261.

Phylogenomic databases

eggNOGCOG0620.
HOGENOMHOG000246221.
KOK00549.
OMASFPQTSE.
OrthoDBEOG6FFS3G.
ProtClustDBPRK05222.

Enzyme and pathway databases

BioCycYPES214092:GKDD-3758-MONOMER.
UniPathwayUPA00051; UER00082.

Family and domain databases

HAMAPMF_00172. Meth_synth.
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETE_YERPE
AccessionPrimary (citable) accession number: Q8ZAL3
Secondary accession number(s): Q0WAM3, Q8D1I5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways