Q8ZAL3 (METE_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	YPO3788, y0442, YP_3261

Organism

Yersinia pestis [Reference proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP-Rule MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP-Rule MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP-Rule MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.
Sequence caution	The sequence AAM84031.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP-Rule MF_00172		PRO_0000098682
Sites
	Metal binding	644	1	Zinc By similarity
	Metal binding	646	1	Zinc By similarity
	Metal binding	729	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ZAL3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 48F4B4D74BB63C3C	FASTA	758	85,677
	10 20 30 40 50 60 MTILNHTLGF PRVGLKRELK KAQESYWAGN STQEELLNVG RELRARHWQQ QQQAGVDLVP 70 80 90 100 110 120 VGDFAWYDHV LTTSLLLGNV PERHQNADGS IDIDTLFRIG RGRAPTGKPA AAAEMTKWFN 130 140 150 160 170 180 TNYHYMVPEF QQGQQFKLGW TQLLDEVDEA LALGHKIKPV LLGPITYLWL GKVKGEQFDR 190 200 210 220 230 240 LSLLNDILPV YQQVLAELAK RGIEWVQIDE PALVLELPQE WLDAYQPAYQ ALQGQVKLLL 250 260 270 280 290 300 TTYFDSIGHN IDTIRALPVQ GLHVDVVTGH DDLAVLNKNL PKEWLLSLGV INGRNVWRAD 310 320 330 340 350 360 LSSWFERLQP LVNSRPLWLG SSCSLLHSPI DLNEETRLDA EVKSWFAFAL QKCAELALLT 370 380 390 400 410 420 QALNAPNDAK LAELAAYSAP IRARRSSSRV HNAQVEQRLA AITSQDIERQ LPYEARAETQ 430 440 450 460 470 480 RKRFNLPAWP TTTIGSFPQT TEIRGLRLDF KQGRLDGKNY RTGISEHIKH AIAEQERLGL 490 500 510 520 530 540 DVLVHGEAER NDMVEYFGEH LDGFVFTQNG WVQSYGSRCV KPPVIIGDIS RPEAITVEWA 550 560 570 580 590 600 KYAQSLTEKP VKGMLTGPVT ILCWSFPRED VSRETIAKQI ALALRDEVED LEKAGIGIIQ 610 620 630 640 650 660 IDEPALREGL PLRRADWQAY LQWAVDAFKL NAAVAQNDTQ IHTHMCYCEF NDIMDSIAAL 670 680 690 700 710 720 DADVITIETS RSDMELLESF EDFAYPNEIG PGVYDIHSPN VPSVEWIEAL LRKAAQRIPA 730 740 750 ERLWVNPDCG LKTRGWPETR QALANMVLAA QRLREEQV

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM10+ / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL22374.1. AE009952 Genomic DNA. Translation: AAM84031.1. Different initiation. AE017042 Genomic DNA. Translation: AAS63429.1.
PIR	AC0461.
RefSeq	NP_667780.1. NC_004088.1. NP_994552.1. NC_005810.1. YP_002348665.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZAL3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8ZAL3. 4 interactions.
STRING	214092.YPO3788.
Proteomic databases
PRIDE	Q8ZAL3.
Protocols and materials databases
DNASU	1145389.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM84031; AAM84031; y0442. AAS63429; AAS63429; YP_3261.
GeneID	1145389. 1176622. 2763568.
KEGG	ype:YPO3788. ypk:y0442. ypm:YP_3261.
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HOG000246221.
KO	K00549.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	YPES214092:GKDD-3758-MONOMER.
UniPathway	UPA00051; UER00082.
Family and domain databases
HAMAP	MF_00172. Meth_synth.
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_YERPE

Accession

Primary (citable) accession number: Q8ZAL3
Secondary accession number(s): Q0WAM3, Q8D1I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program