Q8ZAF0 (WECF_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-alpha-L-fucosyltransferase
EC=2.4.1.-

Alternative name(s):
TDP-Fuc4NAc:lipid II Fuc4NAc transferase
Short name=Fuc4NAc transferase

Gene names

Name:	wecF
Synonyms:	rffT
Ordered Locus Names:	YPO3857, y0371, YP_3188

Organism

Yersinia pestis

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (Lipid III), the third lipid-linked intermediate involved in ECA synthesis By similarity. HAMAP MF_01002
Catalytic activity	TDP-Fuc4NAc + Und-PP-GlcNAc-ManNAcA = TDP + Und-PP-GlcNAc-ManNAcA-Fuc4NAc. HAMAP MF_01002
Pathway	Bacterial outer membrane biogenesis; enterobacterial common antigen biosynthesis. HAMAP MF_01002
Subcellular location	Cell inner membrane; Peripheral membrane protein Potential HAMAP MF_01002.
Sequence similarities	Belongs to the glycosyltransferase 56 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	enterobacterial common antigen biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	4-alpha-L-fucosyltransferase HAMAP MF_01002		PRO_0000216190

Sequences

	Sequence		Length	Mass (Da)
	Q8ZAF0 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: FDA273D7B5A2FD90	FASTA	361	41,071
	10 20 30 40 50 60 MITLTHVLGS DIPHHNLTVL RFFNDVLAKC LPVEQVRHFM VAAKETAPFS SFPQLDINTY 70 80 90 100 110 120 SDKKALAEAV IARAQADRSA RFFWHGQFNV TLWLALLSGK IKPGQVYWHV WGADLYEDAK 130 140 150 160 170 180 SLKFRLFYLL RRIAQGRVGH VFATRGDLIH YQQRHPRVPA SLLYFPTRMD PALTAINIDK 190 200 210 220 230 240 PLAGPMTILV GNSGDTTNRH IEALKAIHQQ FGPDVRVIIP MGYPANNEAY IEQVRQAGLA 250 260 270 280 290 300 LFSQDNLRIL TEQIPFDDYL NILRECDLGY FIFNRQQGIG TLCLLTQFGV PFVLSRKNPF 310 320 330 340 350 360 WQDLAEQHIP VFFYGDTLDE PMIREAQRQL AGLDKQAIAF FNPNYIEGWK QALALAAGEH P

References

[1]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[2]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL590842 Genomic DNA. Translation: CAL22444.1. AE009952 Genomic DNA. Translation: AAM83960.1. AE017042 Genomic DNA. Translation: AAS63356.1.
PIR	AI0469.
RefSeq	NP_667709.1. NC_004088.1. NP_994479.1. NC_005810.1. YP_002348735.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q8ZAF0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8ZAF0. 2 interactions.
Protein family/group databases
CAZy	GT56. Glycosyltransferase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1145318. 1176696. 2764470.
GenomeReviews	Gene locus y0371 in contig AE009952_GR. Gene locus YP_3188 in contig AE017042_GR. Gene locus YPO3857 in contig AL590842_GR.
KEGG	ype:YPO3857. ypk:y0371. ypm:YP_3188.
Phylogenomic databases
HOGENOM	HBG296660.
OMA	IPHHNQT.
ProtClustDB	PRK02797.
Enzyme and pathway databases
BioCyc	YPES187410:Y0371-MONOMER. YPES214092:YPO3857-MONOMER.
Family and domain databases
HAMAP	MF_01002. WecF_RffT. [Tree]
InterPro	IPR009993. 4-alpha-FUT. [Graphical view]
KO	K12582.
Pfam	PF07429. Fuc4NAc_transf. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WECF_YERPE

Accession

Primary (citable) accession number: Q8ZAF0
Secondary accession number(s): Q0WAF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program