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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei405 – 4051NucleophileUniRule annotation
Active sitei458 – 4581Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3917-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:YPO3942, y3886, YP_3304
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815 Componenti: Chromosome UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7277271,4-alpha-glucan branching enzyme GlgBPRO_0000188770Add
BLAST

Proteomic databases

PRIDEiQ8ZA75.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi214092.YPO3942.

Structurei

3D structure databases

ProteinModelPortaliQ8ZA75.
SMRiQ8ZA75. Positions 117-726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ZA75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLPDRQVI NQLISGHYGD PFSILGMHET SQGLQICALL PDAREVWLVE
60 70 80 90 100
TENGRRIAQL TLEDPRGFFI AQLTRRKKSF RYQFAVTWQE NPQIIEDPYR
110 120 130 140 150
FGPLLQDIDS WLLAEGTHLR PYERLGAHLM SLDGVSGVSF AVWAPNAQRV
160 170 180 190 200
SVVGDFNFWD GRRHPMRLRR ENGIWELFLP GIEAGQLYKF EIIDCHGQVR
210 220 230 240 250
LKADPYAFEA QMRPETASLI SPLPDVVKSS AARQKANDLC SPVSIYEVHL
260 270 280 290 300
GSWRRHTDNN FWLSYRELAD QLVEYVKYMG FTHVELLPIN EHPFDGSWGY
310 320 330 340 350
QPLGLYAPTR RYGTPEDFKA FVAKFHQAGI NVILDWVPGH FPSDEHGLST
360 370 380 390 400
FDGTALYEYA DPREGYHQDW NTLIYNYGRN EVRNYLAGNA FYWMERFGID
410 420 430 440 450
ALRIDAVASM IYRDYSRAEG QWVPNYYGGR ENLEAIAFLR YTNKTIGVER
460 470 480 490 500
PGSVTMAEES TDFPGVTLPP DIGGLGFNYK WNMGWMHDTL NYMQCDPVHR
510 520 530 540 550
KYHHNLMTFG MLYAYTENFI LPLSHDEVVH GKRSILDRMP GDAWQKFANL
560 570 580 590 600
RAYYAFMWAH PGKKLLFMGC EFAQGREWNF ETSLDWHLLD DENGWHSGVQ
610 620 630 640 650
RLVRDLNHCY RQYAPLYEWD YQPAGFEWLV VDDHENSVFA FLRRDAEGHE
660 670 680 690 700
LIAISNFTPV PRYHYRVGIP QGGHYREVLN SDSAFYCGSN LGNQGGIDSH
710 720
HVRSHNHEHS LLLTLPPLAT IYLLREN
Length:727
Mass (Da):84,135
Last modified:March 1, 2002 - v1
Checksum:i40D16ED7083B33D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22523.1.
AE009952 Genomic DNA. Translation: AAM87431.1.
AE017042 Genomic DNA. Translation: AAS63469.1.
PIRiAH0479.
RefSeqiNP_671180.1. NC_004088.1.
NP_994592.1. NC_005810.1.
WP_002209500.1. NZ_KN150726.1.
YP_002348813.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87431; AAM87431; y3886.
AAS63469; AAS63469; YP_3304.
GeneIDi1176781.
KEGGiype:YPO3942.
ypk:y3886.
ypm:YP_3304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590842 Genomic DNA. Translation: CAL22523.1.
AE009952 Genomic DNA. Translation: AAM87431.1.
AE017042 Genomic DNA. Translation: AAS63469.1.
PIRiAH0479.
RefSeqiNP_671180.1. NC_004088.1.
NP_994592.1. NC_005810.1.
WP_002209500.1. NZ_KN150726.1.
YP_002348813.1. NC_003143.1.

3D structure databases

ProteinModelPortaliQ8ZA75.
SMRiQ8ZA75. Positions 117-726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi214092.YPO3942.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEiQ8ZA75.

Protocols and materials databases

DNASUi1148833.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87431; AAM87431; y3886.
AAS63469; AAS63469; YP_3304.
GeneIDi1176781.
KEGGiype:YPO3942.
ypk:y3886.
ypm:YP_3304.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciYPES214092:GKDD-3917-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIM10+ / Biovar Mediaevalis.
  3. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiGLGB_YERPE
AccessioniPrimary (citable) accession number: Q8ZA75
Secondary accession number(s): Q0WA75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.