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Protein

Bifunctional aspartokinase/homoserine dehydrogenase

Gene

thrA

Organism
Salmonella typhi
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.UniRule annotation
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Aspartokinase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-homoserine from L-aspartate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Aspartokinase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes L-threonine from L-aspartate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC), Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Aspartokinase (thrA)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Bifunctional aspartokinase/homoserine dehydrogenase (metL), Bifunctional aspartokinase/homoserine dehydrogenase (thrA)
  4. Homoserine kinase (thrB)
  5. no protein annotated in this organism
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, OxidoreductaseUniRule annotation, Transferase
Biological processAmino-acid biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, NADPUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00034; UER00015.
UPA00050; UER00063.
UPA00050; UER00461.
UPA00051; UER00462.
UPA00051; UER00465.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional aspartokinase/homoserine dehydrogenaseUniRule annotation
Including the following 2 domains:
AspartokinaseUniRule annotation (EC:2.7.2.4UniRule annotation)
Homoserine dehydrogenaseUniRule annotation (EC:1.1.1.3UniRule annotation)
Gene namesi
Name:thrAImported
Ordered Locus Names:STY0002Imported, t0002Imported
OrganismiSalmonella typhiImported
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi220341.STY0002.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini320 – 394ACTInterPro annotationAdd BLAST75
Domaini401 – 478ACTInterPro annotationAdd BLAST78

Sequence similaritiesi

In the C-terminal section; belongs to the homoserine dehydrogenase family.UniRule annotation
In the N-terminal section; belongs to the aspartokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CFH. Bacteria.
COG0460. LUCA.
COG0527. LUCA.
HOGENOMiHOG000271593.
KOiK12524.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
InterProiView protein in InterPro
IPR036393. AceGlu_kinase-like_sf.
IPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005106. Asp/hSer_DH_NAD-bd.
IPR001341. Asp_kinase.
IPR018042. Aspartate_kinase_CS.
IPR011147. Bifunc_aspartokin/hSer_DH.
IPR027795. GATS-like_ACT_dom.
IPR001342. HDH_cat.
IPR019811. HDH_CS.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF13840. ACT_7. 2 hits.
PF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
PIRSFiPIRSF000727. ThrA. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.
PS00324. ASPARTOKINASE. 1 hit.
PS01042. HOMOSER_DHGENASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Z9R7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLKFGGTS VANAERFLRV ADILESNSRQ GQVATVLSAP AKITNHLVAM
60 70 80 90 100
IEKTIGGQDA LPNISDAERI FSDLLAGLAS AQPGFPLARL KMVVEQEFAQ
110 120 130 140 150
IKHVLHGISL LGQCPDSINA ALICRGEKMS IAIMAGLLEA RGHRVTVIDP
160 170 180 190 200
VEKLLAVGHY LESTVDIAES TRRIAASQIP ADHMILMAGF TAGNEKGELV
210 220 230 240 250
VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRQV PDARLLKSMS
260 270 280 290 300
YQEAMELSYF GAKVLHPRTI TPIAQFQIPC LIKNTGNPQA PGTLIGASSD
310 320 330 340 350
DDNLPVKGIS NLNNMAMFSV SGPGMKGMIG MAARVFAAMS RAGISVVLIT
360 370 380 390 400
QSSSEYSISF CVPQSDCARA RRAMQDEFYL ELKEGLLEPL AVTERLAIIS
410 420 430 440 450
VVGDGMRTLR GISAKFFAAL ARANINIVAI AQGSSERSIS VVVNNDDATT
460 470 480 490 500
GVRVTHQMLF NTDQVIEVFV IGVGGVGGAL LEQLKRQQTW LKNKHIDLRV
510 520 530 540 550
CGVANSKALL TNVHGLNLDN WQAELAQANA PFNLGRLIRL VKEYHLLNPV
560 570 580 590 600
IVDCTSSQAV ADQYADFLRE GFHVVTPNKK ANTSSMDYYH QLRFAAAQSR
610 620 630 640 650
RKFLYDTNVG AGLPVIENLQ NLLNAGDELQ KFSGILSGSL SFIFGKLEEG
660 670 680 690 700
MSLSQATALA REMGYTEPDP RDDLSGMDVA RKLLILARET GRELELSDIV
710 720 730 740 750
IEPVLPDEFD ASGDVTAFMA HLPQLDDAFA ARVAKARDEG KVLRYVGNIE
760 770 780 790 800
EDGVCRVKIA EVDGNDPLFK VKNGENALAF YSHYYQPLPL VLRGYGAGND
810 820
VTAAGVFADL LRTLSWKLGV
Length:820
Mass (Da):88,712
Last modified:March 1, 2002 - v1
Checksum:iED6CDB2F6F01AC67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014613 Genomic DNA. Translation: AAO67736.1.
AL513382 Genomic DNA. Translation: CAD01155.1.
RefSeqiNP_454612.1. NC_003198.1.
WP_001264731.1. NZ_NIFP01000005.1.

Genome annotation databases

EnsemblBacteriaiAAO67736; AAO67736; t0002.
CAD01155; CAD01155; CAD01155.
CGV60638; CGV60638; ERS248933_01843.
GeneIDi1246513.
KEGGistt:t0002.
sty:STY0002.
PATRICifig|220341.7.peg.1.

Similar proteinsi

Entry informationi

Entry nameiQ8Z9R7_SALTI
AccessioniPrimary (citable) accession number: Q8Z9R7
Secondary accession number(s): A0A0C9EHY8, Q7CBW4
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2002
Last sequence update: March 1, 2002
Last modified: January 31, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported