Q8Z9C3 (PCNB_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase
Short name=PAP
EC=2.7.7.19

Alternative name(s):
Plasmid copy number protein

Gene names

Name:	pcnB
Ordered Locus Names:	STY0209, t0192

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polymerase that creates the 3' poly(A) tail found in some mRNAs. Seems to be involved in plasmid copy number control.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Monomer By similarity.
Miscellaneous	Uses an AUU start codon By similarity.
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.
Sequence caution	The sequence AAO67924.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAD01345.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Plasmid copy control Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA polyadenylation Inferred from electronic annotation. Source: InterPro plasmid maintenance Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 10	10	Potential		PRO_0000411166
	Chain	11 – 465	455	Poly(A) polymerase		PRO_0000139094
Sites
	Active site	80	1	By similarity
	Active site	82	1	By similarity
	Active site	162	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z9C3 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: A94E6DFAFBC83720	FASTA	465	53,902
	10 20 30 40 50 60 MFTRVANFCR KVLSREESEA EQAVARPHMT IIPREQHAIS RKDISENALK VLYRLNKAGY 70 80 90 100 110 120 EAYLVGGGVR DLLLGKKPKD FDVTTNATPD QVRKLFRNCR LVGRRFRLAH VMFGPEIIEV 130 140 150 160 170 180 ATFRGHNEGS ESDRTTSQRG QNGMLLRDNI FGSIEEDAQR RDFTINSLYY SVADFTVRDY 190 200 210 220 230 240 VGGMQDLQEG VIRLIGNPET RYREDPVRML RAVRFAAKLN MRISPETAEP IPRLATLLND 250 260 270 280 290 300 IPPARLFEES LKLLQAGNGF ETYQQLREYH LFQPLFPTIT RYFTENGDSA MERIIAQVLK 310 320 330 340 350 360 NTDNRIRNEM RVNPAFLFAA MFWYPLLEMA QKIAQESGLA YYDAFALAMN DVLDEACRSL 370 380 390 400 410 420 AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LMEHPKFRAA FDLLELRAQV ENNTELQRLA 430 440 450 460 QWWAEFQASA PPEQKGMLNE LDDDPAPRRR RSRPRKRAPR REGTV

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627265 Genomic DNA. Translation: CAD01345.1. Different initiation. AE014613 Genomic DNA. Translation: AAO67924.1. Different initiation.
RefSeq	NP_804075.1. NC_004631.1.
3D structure databases
ProteinModelPortal	Q8Z9C3.
ModBase	Search...
Protein-protein interaction databases
STRING	220341.STY0209.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO67924; AAO67924; t0192. CAD01345; CAD01345; CAD01345.
GeneID	1067783.
KEGG	stt:t0192.
PATRIC	18538240. VBISalEnt120419_0212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0617.
HOGENOM	HOG000256527.
KO	K00970.
OMA	RFMAKLD.
ProtClustDB	PRK11623.
Family and domain databases
InterPro	IPR026007. PcnB/CCA-adding. IPR002646. PolA_pol_head_dom. IPR010206. PolA_pol_PcnB. IPR025866. PolyA_pol_arg_C_dom. [Graphical view]
PANTHER	PTHR13734:SF5. PTHR13734:SF5. 1 hit.
Pfam	PF01743. PolyA_pol. 1 hit. PF12626. PolyA_pol_arg_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01942. pcnB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PCNB_SALTI

Accession

Primary (citable) accession number: Q8Z9C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	September 19, 2006
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program