Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Salmonella typhi
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:STY0237, t0215
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001927651 – 890Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST890

Interactioni

Protein-protein interaction databases

STRINGi220341.STY0237

Structurei

3D structure databases

ProteinModelPortaliQ8Z9A7
SMRiQ8Z9A7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini468 – 590HDPROSITE-ProRule annotationAdd BLAST123
Domaini709 – 790ACT 1UniRule annotationAdd BLAST82
Domaini816 – 890ACT 2UniRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 349UridylyltransferaseAdd BLAST349
Regioni350 – 708Uridylyl-removingAdd BLAST359

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 2 hits
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Z9A7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLPEQHAN TALPTLPDQP QNPGVWPRAE LTVAGIKARI DIFQHWLGEA
60 70 80 90 100
FDSGICAEQL IEARTEFIDQ LLQRLWIEAG FGQIADLALV AVGGYGRGEL
110 120 130 140 150
HPLSDIDLLI LSRKKLPDEQ AQKVGELLTL LWDVKLDVGH SVRTLEECLL
160 170 180 190 200
EGLSDLTVAT NLIETRLLIG DVALFLALQK HIFSEGFWPS DKFYAAKVEE
210 220 230 240 250
QNQRHQRYHG TSYNLEPDIK SSPGGLRDIH TLQWVARRHF GATSLDEMVG
260 270 280 290 300
FGFLTPAECA ELNECLHILW RIRFALHLVV SRYDNRLLFD RQLSVAQRLN
310 320 330 340 350
YSGEGNDPVE RMMKDYFRVT RRVSELNQML LQLFDEAILA LPADEKPRPV
360 370 380 390 400
DDEFQLRGTL IDLRDDTLFI REPQAILRMF YMMVRNSAIT GIYSTTLRHL
410 420 430 440 450
RHARRHLSQP LCYIPEARTL FLSMLRHPGA VSRGLLPMHR HSVLWAYMPQ
460 470 480 490 500
WSHIVGQMQF DLFHAYTVDE HTIRVMLKLE SFAKEETRQR HPLCVDLWPR
510 520 530 540 550
LPHPELILIA ALFHDIAKGR GGDHSVLGAQ DVLTFAELHG LNSRETQLVA
560 570 580 590 600
WLVRQHLLMS VTAQRRDIQD PEVIKQFAEE VQTETRLRFL VCLTVADICA
610 620 630 640 650
TNETLWNSWK QSLLRELYFA TEKQLRRGMQ NTPDMRERVR HHQLQALALL
660 670 680 690 700
RMDNIDEAAL HKIWTRCRAN YFVRHSPNQL AWHARHLLQH DLSQPLVLLS
710 720 730 740 750
PQATRGGTEI FIWSPDRPYL FAAVCAELDR RNLSVHDAQI FTTRDGMAMD
760 770 780 790 800
TFIVLEPDGS PLAADRHDVI RTGLEQTITQ HSWQPPQPRR QPAKLRHFTV
810 820 830 840 850
ETEVNFLPTH TDRKSFMELI ALDQPGLLAR VGQIFADLGI SLHGARITTI
860 870 880 890
GERVEDLFII ATADRRALNN VLQLEVQQRL TAALNPNDKG
Length:890
Mass (Da):102,123
Last modified:March 1, 2002 - v1
Checksum:i0E096D32C17D98DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA Translation: CAD01368.1
AE014613 Genomic DNA Translation: AAO67945.1
RefSeqiNP_454821.1, NC_003198.1
WP_001094518.1, NZ_PHKG01000007.1

Genome annotation databases

EnsemblBacteriaiAAO67945; AAO67945; t0215
CAD01368; CAD01368; CAD01368
GeneIDi1246726
KEGGistt:t0215
sty:STY0237
PATRICifig|220341.7.peg.237

Similar proteinsi

Entry informationi

Entry nameiGLND_SALTI
AccessioniPrimary (citable) accession number: Q8Z9A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health