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Protein

2-methylcitrate dehydratase

Gene

prpD

Organism
Salmonella typhi
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the stereospecific dehydration of (2S,3S)-2-methylcitrate (2-MC) to yield the cis isomer of 2-methyl-aconitate. Could also catalyze the dehydration of citrate and the hydration of cis-aconitate.By similarity

Catalytic activityi

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity
Citrate = isocitrate.By similarity

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. Aconitate hydratase B (acnB), 2-methylcitrate dehydratase (prpD)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718
UPA00946

Names & Taxonomyi

Protein namesi
Recommended name:
2-methylcitrate dehydrataseBy similarity (EC:4.2.1.79By similarity)
Short name:
2-MC dehydrataseBy similarity
Alternative name(s):
(2S,3S)-2-methylcitrate dehydrataseBy similarity
Aconitate hydrataseBy similarity (EC:4.2.1.3By similarity)
Short name:
ACNBy similarity
Short name:
AconitaseBy similarity
Gene namesi
Name:prpD
Ordered Locus Names:STY0402, t2494
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002150251 – 4832-methylcitrate dehydrataseAdd BLAST483

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi220341.STY0402

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MVIX-ray3.05A/B/C/D/E/F1-483[»]
ProteinModelPortaliQ8Z903
SMRiQ8Z903
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PrpD family.Curated

Phylogenomic databases

eggNOGiENOG4105DXD Bacteria
COG2079 LUCA
HOGENOMiHOG000159916
KOiK01720
OMAiECTKHLG

Family and domain databases

InterProiView protein in InterPro
IPR012705 2Me_IsoCit_deHydtase_PrpD
IPR036148 MmgE/PrpD_sf
IPR005656 MmgE_PrpD
PANTHERiPTHR16943 PTHR16943, 1 hit
PfamiView protein in Pfam
PF03972 MmgE_PrpD, 1 hit
SUPFAMiSSF103378 SSF103378, 1 hit
TIGRFAMsiTIGR02330 prpD, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Z903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAHISNVRP DFDREIVDIV DYVMNYEITS KVAYDTAHYC LLDTLGCGLE
60 70 80 90 100
ALEYPACKKL LGPIVPGTVV PNGARVPGTQ FQLDPVQAAF NISAMIRWLD
110 120 130 140 150
FNDTWLAAEW GHPSDNLGGI LATADWLSRN AVAAGKAPLT MKQVLSGMIK
160 170 180 190 200
AHEIQGCIAL ENAFNRVGLD HVLLVKVAST AVVAEMLGLT RDEILNAVSL
210 220 230 240 250
AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK TGEMGYPSAL
260 270 280 290 300
TAKTWGFYDV SFKGETFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA
310 320 330 340 350
MTLYEQMQAA GKTAADIEKV TIRTHEACLR IIDKKGPLNN PADRDHCIQY
360 370 380 390 400
MVAVPLLFGR LTAADYEDEV AQDKRIDALR EKIVCYEDPA FTADYHDPEK
410 420 430 440 450
RAIGNAITVE FTDGSRFGEV VVEYPIGHAR RRADGIPKLI EKFKINLARQ
460 470 480
FLTRQQQRIL DVSLDRARLE QMPVNEYLDL YII
Length:483
Mass (Da):53,770
Last modified:March 1, 2002 - v1
Checksum:i4C695F381E7E228A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti452L → P in AAO70082 (PubMed:12644504).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA Translation: CAD08825.1
AE014613 Genomic DNA Translation: AAO70082.1
RefSeqiNP_454965.1, NC_003198.1
WP_001272709.1, NZ_CJUM01000013.1

Genome annotation databases

EnsemblBacteriaiAAO70082; AAO70082; t2494
CAD08825; CAD08825; CAD08825
GeneIDi1246882
KEGGistt:t2494
sty:STY0402
PATRICifig|220341.7.peg.398

Similar proteinsi

Entry informationi

Entry nameiPRPD_SALTI
AccessioniPrimary (citable) accession number: Q8Z903
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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