Reviewed, UniProtKB/Swiss-Prot Q8Z903 (PRPD_SALTI)

Names and origin

Protein names

Recommended name:
    2-methylcitrate dehydratase
    EC=4.2.1.79

Gene names

Name:	prpD
Ordered Locus Names:	STY0402, t2494

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.
Catalytic activity	(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.
Cofactor	Binds 1 2Fe-2S cluster By similarity.
Pathway	Organic acid metabolism; propanoate degradation.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the prpD family.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	propionate catabolic process, 2-methylcitrate cycle Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 2-methylcitrate dehydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	2-methylcitrate dehydratase		PRO_0000215025
Experimental info
	Sequence conflict	452	1	L → P in AAO70082. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8Z903-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4C695F381E7E228A	FASTA	483	53,770
	10 20 30 40 50 60 MSAHISNVRP DFDREIVDIV DYVMNYEITS KVAYDTAHYC LLDTLGCGLE ALEYPACKKL 70 80 90 100 110 120 LGPIVPGTVV PNGARVPGTQ FQLDPVQAAF NISAMIRWLD FNDTWLAAEW GHPSDNLGGI 130 140 150 160 170 180 LATADWLSRN AVAAGKAPLT MKQVLSGMIK AHEIQGCIAL ENAFNRVGLD HVLLVKVAST 190 200 210 220 230 240 AVVAEMLGLT RDEILNAVSL AWVDGQSLRT YRHAPNTGTR KSWAAGDATS RAVRLALMAK 250 260 270 280 290 300 TGEMGYPSAL TAKTWGFYDV SFKGETFRFQ RPYGSYVMEN VLFKISFPAE FHSQTAVEAA 310 320 330 340 350 360 MTLYEQMQAA GKTAADIEKV TIRTHEACLR IIDKKGPLNN PADRDHCIQY MVAVPLLFGR 370 380 390 400 410 420 LTAADYEDEV AQDKRIDALR EKIVCYEDPA FTADYHDPEK RAIGNAITVE FTDGSRFGEV 430 440 450 460 470 480 VVEYPIGHAR RRADGIPKLI EKFKINLARQ FLTRQQQRIL DVSLDRARLE QMPVNEYLDL YII

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627266 Genomic DNA. Translation: CAD08825.1. AE014613 Genomic DNA. Translation: AAO70082.1.
RefSeq	NP_454965.1.
3D structure databases
SMR	Q8Z903. Positions 11-483.
ModBase	Search...
Genome annotation databases
GeneID	1246882.
GenomeReviews	Gene locus t2494 in contig AE014613_GR. Gene locus STY0402 in contig AL513382_GR.
KEGG	stt:t2494. sty:STY0402.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Z903.
OMA	ALRFPEC.
Enzyme and pathway databases
BioCyc	SENT209261:T2494-MON. SENT220341:STY0402-MON.
BRENDA	4.2.1.79. 3716.
Family and domain databases
InterPro	IPR012705. 2Me_IsoCit_deHydtase_PrpD. IPR005656. MmgE_PrpD. [Graphical view]
PANTHER	PTHR16943. MmgE_PrpD. 1 hit.
Pfam	PF03972. MmgE_PrpD. 1 hit. [Graphical view]
TIGRFAMs	TIGR02330. prpD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRPD_SALTI

Accession

Primary (citable) accession number: Q8Z903

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)