Q8Z8W3 (PANE_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydropantoate 2-reductase
EC=1.1.1.169

Alternative name(s):
Ketopantoate reductase
Short name=KPA reductase
Short name=KPR

Gene names

Name:	panE
Synonyms:	apbA
Ordered Locus Names:	STY0473, t2430

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	2-dehydropantoate 2-reductase		PRO_0000157303
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	176	1	Proton donor
	Binding site	31	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	122	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	256	1	NADP By similarity
Experimental info
	Sequence conflict	117	1	A → T in AAO70019. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8Z8W3 [UniParc]. Last modified February 21, 2002. Version 1. Checksum: 0369C148A42BF583	FASTA	303	33,842
	10 20 30 40 50 60 MKITVLGCGA LGQLWLSALC KHGHDVQGWL RVPQPYCSVN LIDTDGSFFN ESLTANDPDF 70 80 90 100 110 120 LAKSELLLVT LKAWQVSDAV RTLASTLPVT SPILLIHNGM GTIEELQSIQ QPMLMGAITH 130 140 150 160 170 180 AARRDGNIII HVANGTTHIG PAREQDGDYS YLAEILQGVL PDVAWHNNIR AEMWRKLAVN 190 200 210 220 230 240 CVINPLTALW NCPNGELRHH TDEINAICEE VAAVIEREGY HTSADDLCYY VEQVIDSTAE 250 260 270 280 290 300 NISSMLQDVR AMRHTEIDYI TGYLLKRARV HGLAVPENSR LFEMVKRKES EYERSGTGMP RPW

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627266 Genomic DNA. Translation: CAD08890.1. AE014613 Genomic DNA. Translation: AAO70019.1.
RefSeq	NP_806159.1. NC_004631.1.
3D structure databases
ProteinModelPortal	Q8Z8W3.
SMR	Q8Z8W3. Positions 1-293.
ModBase	Search...
Protein-protein interaction databases
STRING	220341.STY0473.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO70019; AAO70019; t2430. CAD08890; CAD08890; CAD08890.
GeneID	1069020.
KEGG	stt:t2430.
PATRIC	18538780. VBISalEnt120419_0474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1893.
HOGENOM	HOG000050223.
KO	K00077.
OMA	PANNTEH.
ProtClustDB	PRK06522.
Enzyme and pathway databases
UniPathway	UPA00028; UER00004.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_SALTI

Accession

Primary (citable) accession number: Q8Z8W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program