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Q8Z8D2

- END8_SALTI

UniProt

Q8Z8D2 - END8_SALTI

Protein

Endonuclease 8

Gene

nei

Organism
Salmonella typhi
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotation

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.UniRule annotation
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAUniRule annotation
    Active sitei3 – 31Proton donorUniRule annotation
    Active sitei53 – 531Proton donor; for beta-elimination activityUniRule annotation
    Binding sitei70 – 701DNAUniRule annotation
    Binding sitei125 – 1251DNAUniRule annotation
    Binding sitei169 – 1691DNAUniRule annotation
    Active sitei253 – 2531Proton donor; for delta-elimination activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8UniRule annotation
    Alternative name(s):
    DNA glycosylase/AP lyase NeiUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
    DNA-(apurinic or apyrimidinic site) lyase NeiUniRule annotation
    Endonuclease VIIIUniRule annotation
    Gene namesi
    Name:neiUniRule annotation
    Ordered Locus Names:STY0771, t2148
    OrganismiSalmonella typhi
    Taxonomic identifieri90370 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000000541: Chromosome, UP000002670: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 263262Endonuclease 8PRO_0000170897Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi220341.STY0771.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8Z8D2.
    SMRiQ8Z8D2. Positions 2-262.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FPG family.UniRule annotation
    Contains 1 FPG-type zinc finger.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0266.
    HOGENOMiHOG000020882.
    KOiK05522.
    OMAiGPDVLDM.
    OrthoDBiEOG6QP131.

    Family and domain databases

    HAMAPiMF_01253. Endonuclease_8.
    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR023713. Endonuclease-VIII.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8Z8D2-1 [UniParc]FASTAAdd to Basket

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    MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF AQLKPYESQL TGQLVTRIET    50
    RGKALLTHFS NGLTLYSHNQ LYGVWRVIDT GEIPQTTRIL RVRLQTADKI 100
    ILLYSASDIE MLTAEQLMTH PFLQRVGPDV LDARLTPEEV KARLLSPRFR 150
    NRQFSGLLLD QSFLAGLGNY LRVEILWQVG LTGQHKAKDL NEAQLNALSH 200
    ALLDIPRLSY TTRGQADENK HHGALFRFKV FHRDGEACER CGGIIEKTTL 250
    SSRPFYWCAH CQK 263
    Length:263
    Mass (Da):29,865
    Last modified:January 23, 2007 - v3
    Checksum:i63257D84FF59BEE2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL513382 Genomic DNA. Translation: CAD05190.1.
    AE014613 Genomic DNA. Translation: AAO69762.1.
    RefSeqiNP_455284.1. NC_003198.1.
    NP_805902.1. NC_004631.1.

    Genome annotation databases

    EnsemblBacteriaiAAO69762; AAO69762; t2148.
    CAD05190; CAD05190; CAD05190.
    GeneIDi1247222.
    KEGGisty:STY0771.
    PATRICi18539403. VBISalEnt120419_0776.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL513382 Genomic DNA. Translation: CAD05190.1 .
    AE014613 Genomic DNA. Translation: AAO69762.1 .
    RefSeqi NP_455284.1. NC_003198.1.
    NP_805902.1. NC_004631.1.

    3D structure databases

    ProteinModelPortali Q8Z8D2.
    SMRi Q8Z8D2. Positions 2-262.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 220341.STY0771.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO69762 ; AAO69762 ; t2148 .
    CAD05190 ; CAD05190 ; CAD05190 .
    GeneIDi 1247222.
    KEGGi sty:STY0771.
    PATRICi 18539403. VBISalEnt120419_0776.

    Phylogenomic databases

    eggNOGi COG0266.
    HOGENOMi HOG000020882.
    KOi K05522.
    OMAi GPDVLDM.
    OrthoDBi EOG6QP131.

    Family and domain databases

    HAMAPi MF_01253. Endonuclease_8.
    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR023713. Endonuclease-VIII.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CT18.
    2. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
      Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
      J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700931 / Ty2.

    Entry informationi

    Entry nameiEND8_SALTI
    AccessioniPrimary (citable) accession number: Q8Z8D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 6, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 93 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3