Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8Z8D2

- END8_SALTI

UniProt

Q8Z8D2 - END8_SALTI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Endonuclease 8

Gene

nei

Organism
Salmonella typhi
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotation

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.UniRule annotation
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNAUniRule annotation
Active sitei3 – 31Proton donorUniRule annotation
Active sitei53 – 531Proton donor; for beta-elimination activityUniRule annotation
Binding sitei70 – 701DNAUniRule annotation
Binding sitei125 – 1251DNAUniRule annotation
Binding sitei169 – 1691DNAUniRule annotation
Active sitei253 – 2531Proton donor; for delta-elimination activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8UniRule annotation
Alternative name(s):
DNA glycosylase/AP lyase NeiUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
DNA-(apurinic or apyrimidinic site) lyase NeiUniRule annotation
Endonuclease VIIIUniRule annotation
Gene namesi
Name:neiUniRule annotation
Ordered Locus Names:STY0771, t2148
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000000541: Chromosome, UP000002670: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 263262Endonuclease 8PRO_0000170897Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi220341.STY0771.

Structurei

3D structure databases

ProteinModelPortaliQ8Z8D2.
SMRiQ8Z8D2. Positions 2-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.UniRule annotation
Contains 1 FPG-type zinc finger.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri229 – 26335FPG-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0266.
HOGENOMiHOG000020882.
KOiK05522.
OMAiGPDVLDM.
OrthoDBiEOG6QP131.

Family and domain databases

HAMAPiMF_01253. Endonuclease_8.
InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8Z8D2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF AQLKPYESQL TGQLVTRIET
60 70 80 90 100
RGKALLTHFS NGLTLYSHNQ LYGVWRVIDT GEIPQTTRIL RVRLQTADKI
110 120 130 140 150
ILLYSASDIE MLTAEQLMTH PFLQRVGPDV LDARLTPEEV KARLLSPRFR
160 170 180 190 200
NRQFSGLLLD QSFLAGLGNY LRVEILWQVG LTGQHKAKDL NEAQLNALSH
210 220 230 240 250
ALLDIPRLSY TTRGQADENK HHGALFRFKV FHRDGEACER CGGIIEKTTL
260
SSRPFYWCAH CQK
Length:263
Mass (Da):29,865
Last modified:January 23, 2007 - v3
Checksum:i63257D84FF59BEE2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL513382 Genomic DNA. Translation: CAD05190.1.
AE014613 Genomic DNA. Translation: AAO69762.1.
RefSeqiNP_455284.1. NC_003198.1.
NP_805902.1. NC_004631.1.

Genome annotation databases

EnsemblBacteriaiAAO69762; AAO69762; t2148.
CAD05190; CAD05190; CAD05190.
GeneIDi1247222.
KEGGisty:STY0771.
PATRICi18539403. VBISalEnt120419_0776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL513382 Genomic DNA. Translation: CAD05190.1 .
AE014613 Genomic DNA. Translation: AAO69762.1 .
RefSeqi NP_455284.1. NC_003198.1.
NP_805902.1. NC_004631.1.

3D structure databases

ProteinModelPortali Q8Z8D2.
SMRi Q8Z8D2. Positions 2-262.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 220341.STY0771.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO69762 ; AAO69762 ; t2148 .
CAD05190 ; CAD05190 ; CAD05190 .
GeneIDi 1247222.
KEGGi sty:STY0771.
PATRICi 18539403. VBISalEnt120419_0776.

Phylogenomic databases

eggNOGi COG0266.
HOGENOMi HOG000020882.
KOi K05522.
OMAi GPDVLDM.
OrthoDBi EOG6QP131.

Family and domain databases

HAMAPi MF_01253. Endonuclease_8.
InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR023713. Endonuclease-VIII.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CT18.
  2. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
    Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
    J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.

Entry informationi

Entry nameiEND8_SALTI
AccessioniPrimary (citable) accession number: Q8Z8D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3