Reviewed, UniProtKB/Swiss-Prot Q8Z728 (MAO1_SALTI)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	STY1494, t1481

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

601 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	NAD-dependent malic enzyme		PRO_0000160230
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	418	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z728-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 0A98386BCEC7FBCE	FASTA	565	62,896
	10 20 30 40 50 60 METTTKKARS LYIPYAGPVL LEFPLLNKGS AFSVEERRNF NLSGLLPEVV ESIEEQAERA 70 80 90 100 110 120 WLQYQGFKTE IDKHIYLRNI QDTNETLFYR LVQNHLEEMM PVIYTPTVGA ACERFSEIYR 130 140 150 160 170 180 RARGVFISYP NRHNMDDILQ NVPNHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 ACGGISPAYT LPVVLDVGTN NQQLLNDPLY MGWRHPRITD DEYYAFVDEF IQAVKQRWPD 250 260 270 280 290 300 ILLQFEDFAQ KNAMPLLTRY RDEICSFNDD IQGTAAVTVG TLIAASRAAG SQLSEQKIVF 310 320 330 340 350 360 LGAGSAGCGI AEQIIAQTQR EGLSEDAARQ NVFMVDRFGL LTDRMPNLLP FQAKLVQKCD 370 380 390 400 410 420 NLQHWDTEND VLSLLDVVRN VKPDILIGVS GQTGLFTEEI IREMHKHCPR PIVMPLSNPT 430 440 450 460 470 480 SRVEATPQDI IAWTEGNALV ATGSPFSPVI WKDKVYPIAQ CNNAYIFPGI GLGVIASGAS 490 500 510 520 530 540 RITDEMLMSA SETLAKHSPL VNNGEGLVLP ALKDIQVVSR AIAFAVGKMA QQQGVAVKTS 550 560 AEALQQAIDD NFWKPEYRDY RRTSI

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627270 Genomic DNA. Translation: CAD01754.1. Different initiation. AE014613 Genomic DNA. Translation: AAO69119.1. Different initiation.
RefSeq	NP_455924.1. NP_805270.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZ3 based on UniProtKB P23368.
ModBase	Search...
Genome annotation databases
GeneID	1070925. 1247883.
GenomeReviews	Gene locus STY1494 in contig AL513382_GR. Gene locus t1481 in contig AE014613_GR.
KEGG	stt:t1481. sty:STY1494.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Z728.
Enzyme and pathway databases
BioCyc	SENT209261:T1481-MON. SENT220341:STY1494-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_SALTI

Accession

Primary (citable) accession number: Q8Z728
Secondary accession number(s): Q7C9R6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	November 25, 2008
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)