Reviewed, UniProtKB/Swiss-Prot Q8Z6X9 (BIOD2_SALTI)

Names and origin

Protein names

Recommended name:
    Probable dethiobiotin synthetase 2
      Short name=Dethiobiotin synthase 2
      Short name=DTB synthetase 2
      Short name=DTBS 2
    EC=6.3.3.3

Gene names

Name:	ynfK
Ordered Locus Names:	STY1575, t1410

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Probable dethiobiotin synthetase 2 HAMAP MF_00336		PRO_0000187986
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z6X9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 434D6D90786BF92F	FASTA	231	24,794
	10 20 30 40 50 60 MLKRFFITGT DTSVGKTVVS RALLQALSSG GKSVAGYKPV AKGSKETPEG MRNKDALVLQ 70 80 90 100 110 120 SVSSLELPYE AINPIALSEE ESSVAHSCPI NYTLLSNGLA SLSDKVDHVV VEGTGGWRSL 130 140 150 160 170 180 MNDLRPLSEW VVQEQLPVLM VVGIQEGCIN HALLTAQAVA NDGLPLIGWV ANRINPGLAH 190 200 210 220 230 YAEIIDVLGK KLPAPLIGEL PYLPRAEQRE LGQYIRLSML GSVLAVDRIM A

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627270 Genomic DNA. Translation: CAD01824.1. AE014613 Genomic DNA. Translation: AAO69054.1.
RefSeq	NP_455990.1. NP_805205.1.
3D structure databases
HSSP	HSSP built from PDB template 1DAI based on UniProtKB P13000.
ModBase	Search...
Genome annotation databases
GeneID	1070846. 1247953.
GenomeReviews	Gene locus t1410 in contig AE014613_GR. Gene locus STY1575 in contig AL513382_GR.
KEGG	stt:t1410. sty:STY1575.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Z6X9.
OMA	ETPEGMR.
Enzyme and pathway databases
BioCyc	SENT209261:T1410-MON. SENT220341:STY1575-MON.
BRENDA	6.3.3.3. 3716.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. IPR002586. CbiA_P_synth. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD2_SALTI

Accession

Primary (citable) accession number: Q8Z6X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)