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Protein

Pyruvate kinase I

Gene

pykF

Organism
Salmonella typhi
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), Probable phosphoglycerate mutase GpmB (gpmB), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pykA), Pyruvate kinase I (pykF)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Metal bindingi34PotassiumBy similarity1
Metal bindingi36PotassiumBy similarity1
Metal bindingi66PotassiumBy similarity1
Metal bindingi67Potassium; via carbonyl oxygenBy similarity1
Sitei220Transition state stabilizerBy similarity1
Metal bindingi222MagnesiumBy similarity1
Binding sitei245Substrate; via amide nitrogenBy similarity1
Metal bindingi246MagnesiumBy similarity1
Binding sitei246Substrate; via amide nitrogenBy similarity1
Binding sitei278SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase I (EC:2.7.1.40)
Alternative name(s):
PK-1
Gene namesi
Name:pykF
Ordered Locus Names:STY1744, t1246
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120711 – 470Pyruvate kinase IAdd BLAST470

Proteomic databases

PRIDEiQ8Z6K2

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi220341.STY1744

Structurei

3D structure databases

ProteinModelPortaliQ8Z6K2
SMRiQ8Z6K2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
HOGENOMiHOG000021559
KOiK00873
OMAiKHEAIEQ

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Z6K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTKIVCTI GPKTESEEML SKMLDAGMNV MRLNFSHGDY AEHGQRIQNL
60 70 80 90 100
RNVMSKTGKK AAILLDTKGP EIRTIKLEGG NDVSLKAGQT FTFTTDKSVV
110 120 130 140 150
GNNEIVAVTY EGFTSDLSVG NTVLVDDGLI GMEVTAIEGN KVICKVLNNG
160 170 180 190 200
DLGENKGVNL PGVSIALPAL AEKDKQDLIF GCEQGVDFVA ASFIRKRSDV
210 220 230 240 250
VEIREHLKAH GGENIQIISK IENQEGLNNF DEILEASDGI MVARGDLGVE
260 270 280 290 300
IPVEEVIFAQ KMMIEKCIRA RKVVITATQM LDSMIKNPRP TRAEAGDVAN
310 320 330 340 350
AILDGTDAVM LSGESAKGKY PLEAVSIMAT ICERTDRVMN SRLDYNNDSR
360 370 380 390 400
KLRITEAVCR GAVETAEKLK APLIVVATQG GKSARAVRKY FPDATILALT
410 420 430 440 450
TNEVTARQLV LSKGVVSQLV KEINSTDDFY RLGKDVALQS GLAQKGDVVV
460 470
MVSGALVPSG TTNTASVHVL
Length:470
Mass (Da):50,656
Last modified:March 1, 2002 - v1
Checksum:iC7BF717504E38262
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti370K → E in AAO68900 (PubMed:12644504).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA Translation: CAD01987.1
AE014613 Genomic DNA Translation: AAO68900.1
RefSeqiNP_456147.1, NC_003198.1

Genome annotation databases

EnsemblBacteriaiAAO68900; AAO68900; t1246
CAD01987; CAD01987; CAD01987
GeneIDi1248116
KEGGistt:t1246
sty:STY1744
PATRICifig|220341.7.peg.1755

Similar proteinsi

Entry informationi

Entry nameiKPYK1_SALTI
AccessioniPrimary (citable) accession number: Q8Z6K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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