ID   Q8Z5S5_SALTI            Unreviewed;       494 AA.
AC   Q8Z5S5; A0A0C9D1I6; A0A3V2QAB5; Q7CAP3;
DT   01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2002, sequence version 1.
DT   27-MAR-2024, entry version 151.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:CAD05711.1, ECO:0000313|EMBL:EBG5392949.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:EBG5392949.1};
GN   Name=amyA {ECO:0000313|EMBL:CAD05711.1};
GN   OrderedLocusNames=STY2171 {ECO:0000313|EMBL:CAD05711.1}, t0914
GN   {ECO:0000313|EMBL:AAO68592.1};
GN   ORFNames=CAJ76_06525 {ECO:0000313|EMBL:EDG8856560.1}, CAJ80_03395
GN   {ECO:0000313|EMBL:EDG8900535.1}, CAJ85_04595
GN   {ECO:0000313|EMBL:EDG8891763.1}, CAL67_12125
GN   {ECO:0000313|EMBL:EDG8933409.1}, CB224_05045
GN   {ECO:0000313|EMBL:EDH5800080.1}, CC884_04690
GN   {ECO:0000313|EMBL:EDI0957133.1}, CC907_12890
GN   {ECO:0000313|EMBL:EDI0994494.1}, CC972_07600
GN   {ECO:0000313|EMBL:EDI1138552.1}, D4Z37_07315
GN   {ECO:0000313|EMBL:EBS6525735.1}, D6Q30_02145
GN   {ECO:0000313|EMBL:EBS1097203.1}, DKA88_09045
GN   {ECO:0000313|EMBL:EBW2552702.1}, DNM71_02850
GN   {ECO:0000313|EMBL:EBV0719297.1}, DNV09_19645
GN   {ECO:0000313|EMBL:EBV1034811.1}, DS261_05465
GN   {ECO:0000313|EMBL:EBX7032981.1}, DUQ91_05470
GN   {ECO:0000313|EMBL:EBS0136369.1}, DUV11_05570
GN   {ECO:0000313|EMBL:EBS5363574.1}, DVG36_04815
GN   {ECO:0000313|EMBL:EBY2833049.1}, EBC38_05325
GN   {ECO:0000313|EMBL:EBZ4081312.1}, EID90_03445
GN   {ECO:0000313|EMBL:EBZ9854009.1}, ELQ62_04415
GN   {ECO:0000313|EMBL:ECA4923261.1}, EU445_05325
GN   {ECO:0000313|EMBL:ECB1384009.1}, EVI00_00960
GN   {ECO:0000313|EMBL:ECB2191408.1}, F9R11_00955
GN   {ECO:0000313|EMBL:EDB3134213.1}, F9Y21_00955
GN   {ECO:0000313|EMBL:EDB3624838.1}, FI137_03315
GN   {ECO:0000313|EMBL:EBG5392949.1}, G2227_00960
GN   {ECO:0000313|EMBL:HAE0479533.1}, G2244_06565
GN   {ECO:0000313|EMBL:HAE0416282.1}, G3982_003934
GN   {ECO:0000313|EMBL:HAE3630203.1}, G3V49_002351
GN   {ECO:0000313|EMBL:HAE1997350.1}, G4I71_001123
GN   {ECO:0000313|EMBL:HAE6053946.1}, G4L28_000796
GN   {ECO:0000313|EMBL:HAE6906261.1}, G4P30_001925
GN   {ECO:0000313|EMBL:HAE7598543.1}, G4Y41_002247
GN   {ECO:0000313|EMBL:HAE9015601.1}, G4Y55_000192
GN   {ECO:0000313|EMBL:HAE9577560.1}, G9264_001041
GN   {ECO:0000313|EMBL:HAF7397662.1}, G9C68_000192
GN   {ECO:0000313|EMBL:HAF0684762.1}, G9X37_003681
GN   {ECO:0000313|EMBL:HAF7363017.1}, GDI29_00955
GN   {ECO:0000313|EMBL:EDH3993716.1}, GND71_001285
GN   {ECO:0000313|EMBL:HAE7809508.1}, K3U68_04595
GN   {ECO:0000313|EMBL:QZA30505.1}, ZZ17_03380
GN   {ECO:0000313|EMBL:ECV8214800.1};
OS   Salmonella typhi.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90370 {ECO:0000313|EMBL:CAD05711.1, ECO:0000313|Proteomes:UP000000541};
RN   [1] {ECO:0000313|EMBL:CAD05711.1, ECO:0000313|Proteomes:UP000000541}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CT18 {ECO:0000313|EMBL:CAD05711.1,
RC   ECO:0000313|Proteomes:UP000000541};
RX   PubMed=11677608; DOI=10.1038/35101607;
RA   Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA   Churcher C., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA   Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A.,
RA   Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T.,
RA   Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A.,
RA   Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.,
RA   Rutherford K., Simmonds M., Skelton J., Stevens K., Whitehead S.,
RA   Barrell B.G.;
RT   "Complete genome sequence of a multiple drug resistant Salmonella enterica
RT   serovar Typhi CT18.";
RL   Nature 413:848-852(2001).
RN   [2] {ECO:0000313|EMBL:AAO68592.1, ECO:0000313|Proteomes:UP000002670}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700931 / Ty2 {ECO:0000313|Proteomes:UP000002670}, and Ty2
RC   {ECO:0000313|EMBL:AAO68592.1};
RX   PubMed=12644504; DOI=10.1128/JB.185.7.2330-2337.2003;
RA   Deng W., Liou S.R., Plunkett G.III., Mayhew G.F., Rose D.J., Burland V.,
RA   Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT   "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and
RT   CT18.";
RL   J. Bacteriol. 185:2330-2337(2003).
RN   [3] {ECO:0000313|EMBL:HAE0416282.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=09-1703 {ECO:0000313|EMBL:HAE3630203.1}, 09-1978
RC   {ECO:0000313|EMBL:HAE6053946.1}, 09-1979
RC   {ECO:0000313|EMBL:HAE7598543.1}, 10-1436
RC   {ECO:0000313|EMBL:HAE1997350.1}, 11-2088
RC   {ECO:0000313|EMBL:HAE6906261.1}, 12-0098
RC   {ECO:0000313|EMBL:HAF7397662.1}, 12-0437
RC   {ECO:0000313|EMBL:HAF7363017.1}, 12-2005
RC   {ECO:0000313|EMBL:HAE9015601.1}, 13-0747
RC   {ECO:0000313|EMBL:HAF0684762.1}, 13-7562
RC   {ECO:0000313|EMBL:HAE9577560.1}, IP E.88.374
RC   {ECO:0000313|EMBL:HAE7809508.1},
RC   Sam_1c96eabc-5ba9-4785-8bb4-b62bc64a6574
RC   {ECO:0000313|EMBL:HAE0416282.1}, and
RC   Sam_2e1fd039-ec06-4964-b74e-443d7665d7a0
RC   {ECO:0000313|EMBL:HAE0479533.1};
RX   PubMed=30286803;
RA   Souvorov A., Agarwala R., Lipman D.J.;
RT   "SKESA: strategic k-mer extension for scrupulous assemblies.";
RL   Genome Biol. 19:153-153(2018).
RN   [4] {ECO:0000313|EMBL:HAE1997350.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=09-1703 {ECO:0000313|EMBL:HAE3630203.1}, 09-1978
RC   {ECO:0000313|EMBL:HAE6053946.1}, 09-1979
RC   {ECO:0000313|EMBL:HAE7598543.1}, 10-1436
RC   {ECO:0000313|EMBL:HAE1997350.1}, 11-2088
RC   {ECO:0000313|EMBL:HAE6906261.1}, 12-0098
RC   {ECO:0000313|EMBL:HAF7397662.1}, 12-0437
RC   {ECO:0000313|EMBL:HAF7363017.1}, 12-2005
RC   {ECO:0000313|EMBL:HAE9015601.1}, 13-0747
RC   {ECO:0000313|EMBL:HAF0684762.1}, 13-7562
RC   {ECO:0000313|EMBL:HAE9577560.1}, IP E.88.374
RC   {ECO:0000313|EMBL:HAE7809508.1},
RC   Sam_1c96eabc-5ba9-4785-8bb4-b62bc64a6574
RC   {ECO:0000313|EMBL:HAE0416282.1}, and
RC   Sam_2e1fd039-ec06-4964-b74e-443d7665d7a0
RC   {ECO:0000313|EMBL:HAE0479533.1};
RG   NCBI Pathogen Detection Project;
RL   Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:EBS0136369.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=116585 {ECO:0000313|EMBL:EDG8933409.1}, 132015
RC   {ECO:0000313|EMBL:EDG8900535.1}, 14549
RC   {ECO:0000313|EMBL:ECV8214800.1}, 186520
RC   {ECO:0000313|EMBL:EDG8891763.1}, 189261
RC   {ECO:0000313|EMBL:EDG8856560.1}, 257355
RC   {ECO:0000313|EMBL:EBW2552702.1}, 338438
RC   {ECO:0000313|EMBL:EDI1138552.1}, 339489
RC   {ECO:0000313|EMBL:EDI0957133.1}, 348081
RC   {ECO:0000313|EMBL:EDI0994494.1}, 365365
RC   {ECO:0000313|EMBL:EDH5800080.1}, 524805
RC   {ECO:0000313|EMBL:EBY2833049.1}, 531284
RC   {ECO:0000313|EMBL:EBV1034811.1}, 539099
RC   {ECO:0000313|EMBL:EBV0719297.1}, 550116
RC   {ECO:0000313|EMBL:EBX7032981.1}, 550675
RC   {ECO:0000313|EMBL:ECB1384009.1}, 550679
RC   {ECO:0000313|EMBL:EBS5363574.1}, 568336
RC   {ECO:0000313|EMBL:EBS0136369.1}, 588927
RC   {ECO:0000313|EMBL:EBS6525735.1}, 606246
RC   {ECO:0000313|EMBL:EBS1097203.1}, 619925
RC   {ECO:0000313|EMBL:EBZ4081312.1}, 643219
RC   {ECO:0000313|EMBL:EBZ9854009.1}, 656788
RC   {ECO:0000313|EMBL:ECA4923261.1}, 673629
RC   {ECO:0000313|EMBL:ECB2191408.1}, 731618
RC   {ECO:0000313|EMBL:EBG5392949.1}, 814947
RC   {ECO:0000313|EMBL:EDB3134213.1}, 815592
RC   {ECO:0000313|EMBL:EDB3624838.1}, and 824494
RC   {ECO:0000313|EMBL:EDH3993716.1};
RA   Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.;
RL   Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000313|EMBL:QZA30505.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ISP2825 {ECO:0000313|EMBL:QZA30505.1};
RA   Lee G.Y., Song J.;
RT   "Complete Genome Sequence of Salmonella enterica Serovar Typhi Strain
RT   ISP2825.";
RL   Submitted (AUG-2021) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR   EMBL; AE014613; AAO68592.1; -; Genomic_DNA.
DR   EMBL; AL513382; CAD05711.1; -; Genomic_DNA.
DR   EMBL; AAFJDY010000003; EBG5392949.1; -; Genomic_DNA.
DR   EMBL; AAGUIA010000004; EBS0136369.1; -; Genomic_DNA.
DR   EMBL; AAGULE010000002; EBS1097203.1; -; Genomic_DNA.
DR   EMBL; AAGVVD010000004; EBS5363574.1; -; Genomic_DNA.
DR   EMBL; AAGWES010000006; EBS6525735.1; -; Genomic_DNA.
DR   EMBL; AAHEBT010000002; EBV0719297.1; -; Genomic_DNA.
DR   EMBL; AAHEEH010000038; EBV1034811.1; -; Genomic_DNA.
DR   EMBL; AAHHUX010000008; EBW2552702.1; -; Genomic_DNA.
DR   EMBL; AAHLZU010000004; EBX7032981.1; -; Genomic_DNA.
DR   EMBL; AAHNRO010000004; EBY2833049.1; -; Genomic_DNA.
DR   EMBL; AAHRAJ010000004; EBZ4081312.1; -; Genomic_DNA.
DR   EMBL; AAHSWZ010000003; EBZ9854009.1; -; Genomic_DNA.
DR   EMBL; AAHUNO010000004; ECA4923261.1; -; Genomic_DNA.
DR   EMBL; AAHWPO010000004; ECB1384009.1; -; Genomic_DNA.
DR   EMBL; AAHWWI010000001; ECB2191408.1; -; Genomic_DNA.
DR   EMBL; AAKUKE010000003; ECV8214800.1; -; Genomic_DNA.
DR   EMBL; AALNBT010000001; EDB3134213.1; -; Genomic_DNA.
DR   EMBL; AALNGB010000001; EDB3624838.1; -; Genomic_DNA.
DR   EMBL; AAMFQS010000005; EDG8856560.1; -; Genomic_DNA.
DR   EMBL; AAMFQZ010000004; EDG8891763.1; -; Genomic_DNA.
DR   EMBL; AAMFRD010000003; EDG8900535.1; -; Genomic_DNA.
DR   EMBL; AAMFRJ010000031; EDG8933409.1; -; Genomic_DNA.
DR   EMBL; AAMHNJ010000001; EDH3993716.1; -; Genomic_DNA.
DR   EMBL; AAMICL010000004; EDH5800080.1; -; Genomic_DNA.
DR   EMBL; AAMJUX010000004; EDI0957133.1; -; Genomic_DNA.
DR   EMBL; AAMJVG010000017; EDI0994494.1; -; Genomic_DNA.
DR   EMBL; AAMJWL010000005; EDI1138552.1; -; Genomic_DNA.
DR   EMBL; DAAQQG010000005; HAE0416282.1; -; Genomic_DNA.
DR   EMBL; DAAQQU010000001; HAE0479533.1; -; Genomic_DNA.
DR   EMBL; DAARDQ010000029; HAE1997350.1; -; Genomic_DNA.
DR   EMBL; DAARRK010000049; HAE3630203.1; -; Genomic_DNA.
DR   EMBL; DAASLV010000008; HAE6053946.1; -; Genomic_DNA.
DR   EMBL; DAASSY010000005; HAE6906261.1; -; Genomic_DNA.
DR   EMBL; DAASYX010000011; HAE7598543.1; -; Genomic_DNA.
DR   EMBL; DAATAS010000005; HAE7809508.1; -; Genomic_DNA.
DR   EMBL; DAATOU010000033; HAE9015601.1; -; Genomic_DNA.
DR   EMBL; DAATPQ010000001; HAE9577560.1; -; Genomic_DNA.
DR   EMBL; DAATYW010000001; HAF0684762.1; -; Genomic_DNA.
DR   EMBL; DAAWCE010000100; HAF7363017.1; -; Genomic_DNA.
DR   EMBL; DAAWDS010000007; HAF7397662.1; -; Genomic_DNA.
DR   EMBL; CP080960; QZA30505.1; -; Genomic_DNA.
DR   RefSeq; NP_456524.1; NC_003198.1.
DR   RefSeq; WP_000795470.1; NZ_WSUR01000004.1.
DR   AlphaFoldDB; Q8Z5S5; -.
DR   STRING; 220341.gene:17586079; -.
DR   KEGG; stt:t0914; -.
DR   KEGG; sty:STY2171; -.
DR   PATRIC; fig|220341.7.peg.2184; -.
DR   eggNOG; COG0366; Bacteria.
DR   HOGENOM; CLU_024572_2_0_6; -.
DR   OMA; CVVIMSN; -.
DR   OrthoDB; 9805159at2; -.
DR   Proteomes; UP000000541; Chromosome.
DR   Proteomes; UP000002670; Chromosome.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR   Gene3D; 2.40.30.140; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Glycosidase {ECO:0000313|EMBL:EBG5392949.1};
KW   Hydrolase {ECO:0000313|EMBL:EBG5392949.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT   DOMAIN          4..402
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        235
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        265
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         104
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         198
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         206
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         239
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   494 AA;  56451 MW;  5D221A77B24F67D4 CRC64;
     MKNPTLLQYF HWYYPDGGKL WSELAERADG LNDIGINMVW LPPACKGASG GYSVGYDSYD
     LFDLGEFDQK GTIATKYGDK RQLLTAIDAL KKNNIAVLLD VVVNHKMGAD EKERIRVQRV
     NQDDRTQIDD NIIECEGWTR YTFPARAGQY SNFIWDYHCF SGIDHIENPD EDGIFKIVND
     YTGDGWNDQV DDELGNFDYL MGENIDFRNH AVTEEIKYWA RWVMEQTHCD GFRLDAVKHI
     PAWFYKEWIE HVQAVAPKPL FIVAEYWSHE VDKLQTYIDQ ADGKTMLFDA PLQMKFHEAS
     RQGAEYDMRH IFTGTLVEAD PFHAVTLVAN HDTQLLQALE APVEPWFKPL AYALILLREN
     GVPSVFYPDL YGASYEDNGE NGETCRVDMP VINQLDRLIL ARQRFAHGIQ TLFFDHPNCI
     AFSRSGTEEN PGCVVVLSNG DDGEKTLLLG DNYANKTWRD FLGNRSEHVV TNDQGEATFF
     CNAGSVSVWV IEDV
//