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Protein

Glucose-1-phosphate cytidylyltransferase

Gene

rfbF

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the tyvelose, a 3,6-dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucleotide donor.1 Publication

Catalytic activityi

CTP + alpha-D-glucose 1-phosphate = diphosphate + CDP-glucose.1 Publication

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Kineticsi

  1. KM=11 µM for CTP-glucose (at pH 8)1 Publication
  2. KM=52 µM for glucose 1-phosphate (at pH 8)1 Publication
  3. KM=67 µM for pyrophosphate (at pH 8)1 Publication
  4. KM=148 µM for CTP (at pH 8)1 Publication
  5. KM=159 µM for UTP (at pH 8)1 Publication
  6. KM=2535 µM for UTP-glucose1 Publication
  7. KM=3000 µM for dCTP (at pH 8)1 Publication
  8. KM=3600 µM for xylose 1-phosphate (at pH 8)1 Publication
  1. Vmax=15.4 µmol/min/mg enzyme toward UTP (at pH 8)1 Publication
  2. Vmax=110.4 µmol/min/mg enzyme toward CTP (at pH 8)1 Publication

Pathwayi: CDP-3,6-dideoxy-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glucose-1-phosphate cytidylyltransferase (rfbF)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. CDP-paratose synthase (rfbS)
  5. CDP-paratose 2-epimerase (rfbE)
This subpathway is part of the pathway CDP-3,6-dideoxy-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate, the pathway CDP-3,6-dideoxy-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23Substrate2 Publications1
Binding sitei104Substrate; via carbonyl oxygen2 Publications1
Binding sitei109Substrate2 Publications1
Binding sitei128Substrate; via amide nitrogen2 Publications1
Metal bindingi129Magnesium1 Publication1
Metal bindingi234Magnesium1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ8Z5I4.
UniPathwayiUPA00055; UER00511.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate cytidylyltransferase (EC:2.7.7.33)
Alternative name(s):
CDP-glucose pyrophosphorylase
Gene namesi
Name:rfbF
Ordered Locus Names:STY2302, t0780
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003894461 – 257Glucose-1-phosphate cytidylyltransferaseAdd BLAST257

Interactioni

Subunit structurei

Homohexamer.2 Publications

Protein-protein interaction databases

STRINGi220341.STY2302.

Structurei

Secondary structure

1257
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi12 – 14Combined sources3
Helixi15 – 18Combined sources4
Helixi23 – 25Combined sources3
Helixi33 – 43Combined sources11
Beta strandi48 – 53Combined sources6
Helixi57 – 65Combined sources9
Helixi67 – 71Combined sources5
Beta strandi74 – 77Combined sources4
Helixi78 – 80Combined sources3
Beta strandi82 – 85Combined sources4
Beta strandi94 – 99Combined sources6
Helixi106 – 112Combined sources7
Helixi113 – 116Combined sources4
Turni117 – 119Combined sources3
Beta strandi123 – 127Combined sources5
Beta strandi130 – 132Combined sources3
Helixi136 – 146Combined sources11
Beta strandi149 – 155Combined sources7
Beta strandi161 – 167Combined sources7
Beta strandi170 – 177Combined sources8
Beta strandi190 – 192Combined sources3
Helixi194 – 199Combined sources6
Turni207 – 209Combined sources3
Helixi210 – 217Combined sources8
Beta strandi221 – 226Combined sources6
Beta strandi230 – 232Combined sources3
Helixi236 – 247Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TZFX-ray2.10A2-257[»]
1WVCX-ray2.50A2-257[»]
ProteinModelPortaliQ8Z5I4.
SMRiQ8Z5I4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8Z5I4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 10Substrate binding5
Regioni11 – 13Substrate binding3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EJZ. Bacteria.
COG1208. LUCA.
HOGENOMiHOG000283474.
KOiK00978.
OMAiYSEPWRV.

Family and domain databases

CDDicd02524. G1P_cytidylyltransferase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR013446. G1P_cyt_trans.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF16. PTHR22572:SF16. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02623. G1P_cyt_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Z5I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVILAGGL GTRLSEETIV KPKPMVEIGG KPILWHIMKM YSVHGIKDFI
60 70 80 90 100
ICCGYKGYVI KEYFANYFLH MSDVTFHMAE NRMEVHHKRV EPWNVTLVDT
110 120 130 140 150
GDSSMTGGRL KRVAEYVKDD EAFLFTYGDG VADLDIKATI DFHKAHGKKA
160 170 180 190 200
TLTATFPPGR FGALDIQAGQ VRSFQEKPKG DGAMINGGFF VLNPSVIDLI
210 220 230 240 250
DNDATTWEQE PLMTLAQQGE LMAFEHPGFW QPMDTLRDKV YLEGLWEKGK

APWKTWE
Length:257
Mass (Da):29,007
Last modified:March 1, 2002 - v1
Checksum:i2B0B30E61067E40D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014613 Genomic DNA. Translation: AAO68471.1.
AL513382 Genomic DNA. Translation: CAD02455.1.
RefSeqiNP_456641.1. NC_003198.1.
WP_000648783.1. NZ_LUHL01000038.1.

Genome annotation databases

EnsemblBacteriaiAAO68471; AAO68471; t0780.
CAD02455; CAD02455; CAD02455.
GeneIDi1248637.
KEGGistt:t0780.
sty:STY2302.
PATRICi18542545. VBISalEnt120419_2322.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014613 Genomic DNA. Translation: AAO68471.1.
AL513382 Genomic DNA. Translation: CAD02455.1.
RefSeqiNP_456641.1. NC_003198.1.
WP_000648783.1. NZ_LUHL01000038.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TZFX-ray2.10A2-257[»]
1WVCX-ray2.50A2-257[»]
ProteinModelPortaliQ8Z5I4.
SMRiQ8Z5I4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY2302.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO68471; AAO68471; t0780.
CAD02455; CAD02455; CAD02455.
GeneIDi1248637.
KEGGistt:t0780.
sty:STY2302.
PATRICi18542545. VBISalEnt120419_2322.

Phylogenomic databases

eggNOGiENOG4105EJZ. Bacteria.
COG1208. LUCA.
HOGENOMiHOG000283474.
KOiK00978.
OMAiYSEPWRV.

Enzyme and pathway databases

UniPathwayiUPA00055; UER00511.
UPA00281.
SABIO-RKQ8Z5I4.

Miscellaneous databases

EvolutionaryTraceiQ8Z5I4.

Family and domain databases

CDDicd02524. G1P_cytidylyltransferase. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR013446. G1P_cyt_trans.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF16. PTHR22572:SF16. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02623. G1P_cyt_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFBF_SALTI
AccessioniPrimary (citable) accession number: Q8Z5I4
Secondary accession number(s): Q7CAW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Catalysis proceeds via a sequential rather than a ping-pong bi-bi reaction mechanism.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.