Reviewed, UniProtKB/Swiss-Prot Q8Z4K0 (NADB_SALTI)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	STY2834, t0269

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	L-aspartate oxidase		PRO_0000184399
Regions
	Nucleotide binding	12 – 26	15	FAD Potential
Sites
	Active site	244	1	By similarity
	Active site	263	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z4K0-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 342785A756623204	FASTA	540	60,162
	10 20 30 40 50 60 MMTTPELSCD VLIIGSGAAG LSLALRLAEK HKVIVLSKGP VSEGSTFYAQ GGIAAVFDET 70 80 90 100 110 120 DSIASHVEDT LIAGAGICDR HAVEFVASNA RTCVQWLIDQ GVLFDTHVQP NGKESYHLTR 130 140 150 160 170 180 EGGHSHRRIL HAADATGKEV ETTLVSRAQN HPNIQVLERS NAVDLIISDK IGLPGPRRVV 190 200 210 220 230 240 GAWIWNRNKE WVETCHAKSV VLATGGASKV YQYTTNPDIS SGDGIAMAWR AGCRVANLEF 250 260 270 280 290 300 NQFHPTALYH PQARNFLLTE ALRGEGAYLK RPDGSRFMPD VDERGELAPR DIVARAIDHE 310 320 330 340 350 360 MKRLGADCMF LDISHKPDDF VRQHFPMIYA KLLDLGMDLT KEPIPIVPAA HYTCGGVVVD 370 380 390 400 410 420 DYGRTDVDGL YAIGEVSYTG LHGANRMASN SLLECLVYGW SAAMDIDRRM PSVHSVDVLP 430 440 450 460 470 480 AWDESRVENA DERVVIQHNW HELRLLMWDY VGIVRTTKRL ERALRRITML QQEIDEYYAN 490 500 510 520 530 540 FRVSNNLLEL RNLVQVAELI VRCAMMRKES RGLHFTLDYP QQLAESGPSI LSPLTPHINR

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627275 Genomic DNA. Translation: CAD02790.1. AE014613 Genomic DNA. Translation: AAO67994.1.
RefSeq	NP_457117.1. NP_804145.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
SMR	Q8Z4K0. Positions 3-533.
ModBase	Search...
Genome annotation databases
GeneID	1067870. 1249143.
GenomeReviews	Gene locus t0269 in contig AE014613_GR. Gene locus STY2834 in contig AL513382_GR.
KEGG	stt:t0269. sty:STY2834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Z4K0.
OMA	Q8Z4K0. GAYIWNR.
Enzyme and pathway databases
BioCyc	SENT209261:T0269-MON. SENT220341:STY2834-MON.
BRENDA	1.4.3.16. 3716.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_SALTI

Accession

Primary (citable) accession number: Q8Z4K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)