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Protein

S-ribosylhomocysteine lyase

Gene

luxS

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).UniRule annotation

Catalytic activityi

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54IronUniRule annotation1
Metal bindingi58IronUniRule annotation1
Metal bindingi128IronUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAutoinducer synthesis, Quorum sensing
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
S-ribosylhomocysteine lyaseUniRule annotation (EC:4.4.1.21UniRule annotation)
Alternative name(s):
AI-2 synthesis proteinUniRule annotation
Autoinducer-2 production protein LuxSUniRule annotation
Gene namesi
Name:luxSUniRule annotation
Ordered Locus Names:STY2943, t2714
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001722482 – 171S-ribosylhomocysteine lyaseAdd BLAST170

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi220341.STY2943.

Structurei

Secondary structure

1171
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 26Combined sources11
Beta strandi32 – 39Combined sources8
Turni43 – 45Combined sources3
Helixi50 – 68Combined sources19
Beta strandi70 – 72Combined sources3
Beta strandi74 – 80Combined sources7
Beta strandi84 – 93Combined sources10
Helixi97 – 112Combined sources16
Helixi117 – 119Combined sources3
Turni125 – 127Combined sources3
Turni129 – 132Combined sources4
Helixi136 – 149Combined sources14
Helixi156 – 159Combined sources4
Helixi163 – 168Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E68X-ray1.58A/B1-171[»]
ProteinModelPortaliQ8Z4D7.
SMRiQ8Z4D7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LuxS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106762. Bacteria.
COG1854. LUCA.
HOGENOMiHOG000040371.
KOiK07173.

Family and domain databases

Gene3Di3.30.1360.80. 1 hit.
HAMAPiMF_00091. LuxS. 1 hit.
InterProiView protein in InterPro
IPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
PfamiView protein in Pfam
PF02664. LuxS. 1 hit.
PIRSFiPIRSF006160. AI2. 1 hit.
PRINTSiPR01487. LUXSPROTEIN.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD013172. S-ribosylhomocysteinase. 1 hit.
SUPFAMiSSF63411. SSF63411. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8Z4D7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLDSFAVD HTRMQAPAVR GAKTMNTPHG DAITVFDLRF CIPNKEVMPE
60 70 80 90 100
KGIHTLEHLF AGFMRDHLNG NGVEIIDISP MGCRTGFYMS LIGTPDEQRV
110 120 130 140 150
ADAWKAAMAD VLKVQDQNQI PELNVYQCGT YQMHSLSEAQ DIARHILERD
160 170
VRVNSNKELA LPKEKLQELH I
Length:171
Mass (Da):19,266
Last modified:January 23, 2007 - v3
Checksum:i653B89330FE461A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21G → V in AAO70280 (PubMed:12644504).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA. Translation: CAD05928.1.
AE014613 Genomic DNA. Translation: AAO70280.1.
RefSeqiNP_457215.1. NC_003198.1.

Genome annotation databases

EnsemblBacteriaiAAO70280; AAO70280; t2714.
CAD05928; CAD05928; CAD05928.
GeneIDi1249250.
KEGGistt:t2714.
sty:STY2943.
PATRICifig|220341.7.peg.2996.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA. Translation: CAD05928.1.
AE014613 Genomic DNA. Translation: AAO70280.1.
RefSeqiNP_457215.1. NC_003198.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5E68X-ray1.58A/B1-171[»]
ProteinModelPortaliQ8Z4D7.
SMRiQ8Z4D7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY2943.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO70280; AAO70280; t2714.
CAD05928; CAD05928; CAD05928.
GeneIDi1249250.
KEGGistt:t2714.
sty:STY2943.
PATRICifig|220341.7.peg.2996.

Phylogenomic databases

eggNOGiENOG4106762. Bacteria.
COG1854. LUCA.
HOGENOMiHOG000040371.
KOiK07173.

Family and domain databases

Gene3Di3.30.1360.80. 1 hit.
HAMAPiMF_00091. LuxS. 1 hit.
InterProiView protein in InterPro
IPR011249. Metalloenz_LuxS/M16.
IPR003815. S-ribosylhomocysteinase.
PfamiView protein in Pfam
PF02664. LuxS. 1 hit.
PIRSFiPIRSF006160. AI2. 1 hit.
PRINTSiPR01487. LUXSPROTEIN.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD013172. S-ribosylhomocysteinase. 1 hit.
SUPFAMiSSF63411. SSF63411. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLUXS_SALTI
AccessioniPrimary (citable) accession number: Q8Z4D7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 98 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.