Reviewed, UniProtKB/Swiss-Prot Q8Z460 (CYSH_SALTI)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	STY3074, t2847

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

601 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite.
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 244	243	Phosphoadenosine phosphosulfate reductase		PRO_0000100642

Sequences

	Sequence		Length	Mass (Da)
	Q8Z460-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8B08DCF029CE141D	FASTA	244	27,938
	10 20 30 40 50 60 MSQLDLNALN ELPKVDRVLA LAETNAQLET LTAEERVAWA LENLPGEYVL SSSFGIQAAV 70 80 90 100 110 120 SLHLVNQIRP DIPVILTDTG YLFPETYQFI DELTDKLKLN LKVYRAGESP AWQEARYGKL 130 140 150 160 170 180 WEQGVEGIEK YNEINKVEPM NRALKELNAQ TWFAGLRREQ SGSRAHLPVL AIQRGVFKVL 190 200 210 220 230 240 PIIDWDNRTV YQYLQKHGLK YHPLWAQGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627276 Genomic DNA. Translation: CAD06052.1. AE014613 Genomic DNA. Translation: AAO70404.1.
RefSeq	NP_457335.1. NP_806544.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUR based on UniProtKB P17854.
SMR	Q8Z460. Positions 2-230.
ModBase	Search...
Genome annotation databases
GeneID	1068742. 1249374.
GenomeReviews	Gene locus STY3074 in contig AL513382_GR. Gene locus t2847 in contig AE014613_GR.
KEGG	stt:t2847. sty:STY3074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Z460.
Enzyme and pathway databases
BioCyc	SENT209261:T2847-MON. SENT220341:STY3074-MON.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_SALTI

Accession

Primary (citable) accession number: Q8Z460

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 46 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)