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Q8Z3X8 (SYK1_SALTI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase

EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS
Gene names
Name:lysS
Ordered Locus Names:STY3196, t2958
OrganismSalmonella typhi [Complete proteome] [HAMAP]
Taxonomic identifier90370 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252

Cofactor

Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00252

Subunit structure

Homodimer By similarity. HAMAP MF_00252

Subcellular location

Cytoplasm HAMAP MF_00252.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processlysyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

lysine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 505504Lysine--tRNA ligase HAMAP MF_00252
PRO_0000152625

Sites

Metal binding4151Magnesium 1 By similarity
Metal binding4221Magnesium 1 By similarity
Metal binding4221Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Z3X8 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8E3B55C3BB792FB8

FASTA50557,556
        10         20         30         40         50         60 
MSEQNAQGAD EVVDLNNEMK ARREKLAALR EQGIPFPNDF RRDRTSDQLH AEFDAKEAEE 

        70         80         90        100        110        120 
LEALNIEVSV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA RDDLPEGVYN EQFKKWDLGD 

       130        140        150        160        170        180 
ILGAKGKLFK TKTGELSIHC TELRLLTKAL RPLPDKFHGL QDQEARYRQR YLDLISNDES 

       190        200        210        220        230        240 
RNTFKTRSKI LAGIRQFMVA RGFMEVETPM MQVIPGGASA RPFITHHNAL DLDMYLRIAP 

       250        260        270        280        290        300 
ELYLKRLVVG GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYKDL IELTESLFRT 

       310        320        330        340        350        360 
LAQDVLGTTQ VPYGDEVFDF GKPFEKLTMH EAIKKYRPET DMADLDNFDS AKAIAESIGI 

       370        380        390        400        410        420 
HVEKSWGLGR IVTEIFDEVA EAHLIQPTFI TEYPAEVSPL ARRNDVNPEI TDRFEFFIGG 

       430        440        450        460        470        480 
REIGNGFSEL NDAEDQAQRF LDQVNAKAAG DDEAMFYDED YVTALEHGLP PTAGLGIGID 

       490        500 
RMVMLFTNSH TIRDVILFPA MRPVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL627277 Genomic DNA. Translation: CAD02870.1.
AE014613 Genomic DNA. Translation: AAO70510.1.
RefSeqNP_457438.1. NC_003198.1.
NP_806650.1. NC_004631.1.

3D structure databases

ProteinModelPortalQ8Z3X8.
SMRQ8Z3X8. Positions 12-503.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1068608.
1249480.
GenomeReviewsGene locus t2958 in contig AE014613_GR.
Gene locus STY3196 in contig AL513382_GR.
KEGGstt:t2958.
sty:STY3196.
PATRIC18544456. VBISalEnt120419_3254.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG631383.
OMAVEHKLEQ.
ProtClustDBPRK00484.

Enzyme and pathway databases

BioCycSENT209261:T2958-MONOMER.
SENT220341:STY3196-MONOMER.

Family and domain databases

HAMAPMF_00252. Lys_tRNA_synth_class2.
[Tree]
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-synth_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK04567.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00499. LysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK1_SALTI
AccessionPrimary (citable) accession number: Q8Z3X8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 67 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families