Q8Z3G5 (KDSC_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC
EC=3.1.3.45

Alternative name(s):
KDO 8-P phosphatase

Gene names

Name:	kdsC
Ordered Locus Names:	STY3495, t3233

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipopolysaccharides (LPSs). Catalyzes the hydrolysis of 3-deoxy-D-manno-octulosonate 8-phosphate (KDO 8-P) to 3-deoxy-D-manno-octulosonate (KDO) and inorganic phosphate By similarity.
Catalytic activity	3-deoxy-D-manno-octulosonate 8-phosphate + H2O = 3-deoxy-D-manno-octulosonate + phosphate.
Cofactor	Magnesium Probable.
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 3/3. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the kdsC family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-manno-octulosonate-8-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC		PRO_0000201699
Regions
	Region	32 – 34	3	Substrate binding By similarity
	Region	55 – 59	5	Substrate binding By similarity
	Region	76 – 77	2	Substrate binding By similarity
Sites
	Metal binding	32	1	Magnesium By similarity
	Metal binding	34	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	125	1	Magnesium By similarity
	Binding site	34	1	Substrate By similarity
	Binding site	63	1	Substrate By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	187	1	Substrate; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z3G5 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: ADEFB0323DBFEF43	FASTA	188	20,075
	10 20 30 40 50 60 MSKAGASLAT CYGPVSTHVM TKAENIRLLI LDVDGVLSDG LIYMGNNGEE LKAFNVRDGY 70 80 90 100 110 120 GIRCALTSNI EVAIITGRKA KLVEDRCATL GIVHLYQGQS NKLIAFSDLL EKLAIAPENV 130 140 150 160 170 180 AYVGDDLIDW PVMEKVGLSV AVADAHPLLI PRADYVTHIA GGRGAVREVC DLLLLAQGKL DEAKGQSI

References

[1]	"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.
[2]	"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627278 Genomic DNA. Translation: CAD07833.1. AE014613 Genomic DNA. Translation: AAO70769.1.
RefSeq	NP_457695.1. NC_003198.1. NP_806909.1. NC_004631.1.
3D structure databases
ProteinModelPortal	Q8Z3G5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1068270. 1249751.
GenomeReviews	Gene locus t3233 in contig AE014613_GR. Gene locus STY3495 in contig AL513382_GR.
KEGG	stt:t3233. sty:STY3495.
PATRIC	18545078. VBISalEnt120419_3559.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG471796.
OMA	KTFNTLD.
ProtClustDB	PRK09484.
Enzyme and pathway databases
BioCyc	SENT209261:T3233-MONOMER. SENT220341:STY3495-MONOMER.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR013200. HAD-SF_hydro-like_3. IPR010023. KDO_8-P_phosphatase. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K03270.
PANTHER	PTHR26053. PTHR26053. 1 hit.
Pfam	PF08282. Hydrolase_3. 1 hit. [Graphical view]
PIRSF	PIRSF006118. KDO8-P_Ptase. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01670. YrbI-phosphatas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSC_SALTI

Accession

Primary (citable) accession number: Q8Z3G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program