Reviewed, UniProtKB/Swiss-Prot Q8Z2M4 (TORA_SALTI)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase
      Short name=TMAO reductase
      Short name=Trimethylamine oxidase
    EC=1.7.2.3

Gene names

Name:	torA
Ordered Locus Names:	STY3956, t3696

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	850 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Tat-type signal Potential
	Chain	40 – 850	811	Trimethylamine-N-oxide reductase		PRO_0000019155

Sequences

	Sequence		Length	Mass (Da)
	Q8Z2M4-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 06B76E03E1FCC987	FASTA	850	94,675
	10 20 30 40 50 60 MKNKDSLHVS RRRFLAQLGG LTVAGMLGPS LLTPRSARAA DAVAPGAATK EGILTGSHWG 70 80 90 100 110 120 AIRATVVDGR FVAAKPFEQD KYPSKMIAGL PDHVHNAARI RYPMVRVDWM RKGHQSDTSQ 130 140 150 160 170 180 RGDNRFVRVS WDEALDLFYQ ELERVQKTYG PSALLTASGW QSTGMFHNAS GMLARAIALH 190 200 210 220 230 240 GNSVSTGGDY STGAAQVILP RVVGSMEVYE QQTSWPLVLQ NSKTIVLWGS DMVKNQQANW 250 260 270 280 290 300 WCPDHDVYQY YEQLKEKVAS GAISVISIDP VVTSTHDYLG RDKVKHIAIN PQTDVPLQLA 310 320 330 340 350 360 LAHTLYSEKL YDKNFLDNYC VGFDQFLPYL LGEKDGQPKD AAWAEKLCGI DADTIRALAR 370 380 390 400 410 420 QMAGDRTQII AGWCVQRMQH GEQWSWMVVV LAAMLGQIGL PGGGFGFGWH YNGAGTPGRK 430 440 450 460 470 480 GIILSGFSGS TTVPPVHDST DYKGYSSTIP IARFMDAILE PGKIINWNGK SVKLPPLKMC 490 500 510 520 530 540 VFAGTNPFHR HQQINRIIEG WRKLETVIAI DNQWTSTCRF ADIVLPATTQ FERNDLDQFG 550 560 570 580 590 600 NHSNRGIIAM KQVVSPQFEA RNDFDIFRDL CRRFNREAAF TEGLDEMGWL KRIWQEGSQQ 610 620 630 640 650 660 GKGRGIHLPT FEVFWNQQEY IEFDHPQMFV RHQAFREDPD LEPLGTPSGL IEIYSKTIAD 670 680 690 700 710 720 MQYDDCQGHP MWFEKIERSH GGPGSQRWPL HLQSVHPDFR LHSQLCESET LRQQYAVGGK 730 740 750 760 770 780 EPVFINPQDA SARGIRNGDI VRVFNARGQV LAGAVVSDRY APGVARIHEG AWYDPDKGGD 790 800 810 820 830 840 LNALCKYGNP NVLTLDTGTS QLAQATSAHT TLVEIEKYTG PMDNVTAFNG PAEMVAQCEY 850 VPASQGNPHD

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627280 Genomic DNA. Translation: CAD03172.1. AE014613 Genomic DNA. Translation: AAO71191.1.
RefSeq	NP_458117.1. NP_807331.1.
3D structure databases
SMR	Q8Z2M4. Positions 52-834.
ModBase	Search...
Genome annotation databases
GeneID	1068717. 1250203.
GenomeReviews	Gene locus t3696 in contig AE014613_GR.
KEGG	stt:t3696. sty:STY3956.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304064.
OMA	QQYAVGG.
Enzyme and pathway databases
BioCyc	SENT209261:T3696-MONOMER. SENT220341:STY3956-MONOMER.
BRENDA	1.7.2.3. 3716.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006658. BisC. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006311. TAT_signal. IPR011887. TorA. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR00509. bisC_fam. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. TIGR02164. torA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORA_SALTI

Accession

Primary (citable) accession number: Q8Z2M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)