Q8Z201 (CYSG_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Siroheme synthase

Including the following 3 domains:

1. Uroporphyrinogen-III C-methyltransferase
   Short name=Urogen III methylase
   EC=2.1.1.107
   Alternative name(s):
   SUMT
   Uroporphyrinogen III methylase
   Short name=UROM
2. Precorrin-2 dehydrogenase
   EC=1.3.1.76
3. Sirohydrochlorin ferrochelatase
   EC=4.99.1.4

Gene names

Name:	cysG
Ordered Locus Names:	STY4319, t4028

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP MF_01646
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646 S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646 Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP MF_01646 Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP MF_01646
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	NAD S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW siroheme biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: EC sirohydrochlorin ferrochelatase activity Inferred from electronic annotation. Source: EC uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Siroheme synthase HAMAP MF_01646		PRO_0000330556
Regions
	Region	218 – 457	240	Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

Sequences

	Sequence		Length	Mass (Da)
	Q8Z201 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 8DFDA9C060D9FE4F	FASTA	457	50,170
	10 20 30 40 50 60 MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLEAGARLTV NALTFIPQFT VWVNEGMLTL 70 80 90 100 110 120 VEGPFDETLL DSCWLAIAAT DDDTVNQRVS EAAESRRIFC NVVDAPKAAS FIMPSIIDRS 130 140 150 160 170 180 PLMVAVSSGG TSPVLARLLR EKLESLLPQH LGQVARYAGQ LRARVKKQFA TMGERRRFWE 190 200 210 220 230 240 KFFVNDRLAQ SLANADEKAV NATTEHLFSE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ 250 260 270 280 290 300 QADIVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK 310 320 330 340 350 360 GGDPFIFGRG GEELETLCHA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA QSVRLVTGHL 370 380 390 400 410 420 KTGGELDWEN LAAEKQTLVF YMGLNQAATI QEKLIAFGME ANMPVALVEN GTSVKQRVVH 430 440 450 GVLTQLGELA QQVESPALII VGRVVALRDK LNWFSNH

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014613 Genomic DNA. Translation: AAO71497.1. AL627281 Genomic DNA. Translation: CAD08136.1.
RefSeq	NP_458425.1. NC_003198.1. NP_807637.1. NC_004631.1.
3D structure databases
HSSP	HSSP built from PDB template 1PJQ based on UniProtKB P25924.
ProteinModelPortal	Q8Z201.
SMR	Q8Z201. Positions 1-457.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1069890. 1250535.
GenomeReviews	Gene locus t4028 in contig AE014613_GR. Gene locus STY4319 in contig AL513382_GR.
KEGG	stt:t4028. sty:STY4319.
PATRIC	18546861. VBISalEnt120419_4414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG730212.
OMA	MCRRDAE.
ProtClustDB	PRK10637.
Enzyme and pathway databases
BioCyc	SENT209261:T4028-MONOMER. SENT220341:STY4319-MONOMER.
Family and domain databases
HAMAP	MF_01646. Siroheme_synth. [Tree]
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA_cysG_C. IPR016040. NAD(P)-bd_dom. IPR012409. Sirohaem_synth. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit. G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.8.210. Sirohaem_synthase_dimer_dom. 1 hit.
KO	K02302.
Pfam	PF10414. CysG_dimeriser. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
PIRSF	PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAM	SSF53790. Cor/por_Metransf. 1 hit.
TIGRFAMs	TIGR01469. CobA_cysG_Cterm. 1 hit. TIGR01470. CysG_Nterm. 1 hit.
PROSITE	PS00839. SUMT_1. 1 hit. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSG_SALTI

Accession

Primary (citable) accession number: Q8Z201
Secondary accession number(s): Q7C5U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program