Reviewed, UniProtKB/Swiss-Prot Q8Z1A8 (DSBD_SALTI)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	STY4682, t4374

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 567	548	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_0000007385
Regions
	Transmembrane	170 – 192	23	Potential
	Transmembrane	212 – 234	23	Potential
	Transmembrane	246 – 268	23	Potential
	Transmembrane	297 – 319	23	Potential
	Transmembrane	326 – 348	23	Potential
	Transmembrane	358 – 380	23	Potential
	Transmembrane	387 – 409	23	Potential
	Domain	435 – 567	133	Thioredoxin
Amino acid modifications
	Disulfide bond	122 ↔ 128		Redox-active By similarity
	Disulfide bond	185 ↔ 307		Redox-active By similarity
	Disulfide bond	482 ↔ 485		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z1A8-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: CF995E0F1AF9F7C9	FASTA	567	61,356
	10 20 30 40 50 60 MAQRIFTLIL LLCSTSAFAG LFDAPGRSQF VPADRAFVFD FQQNQHDLTL SWQVKEGYYL 70 80 90 100 110 120 YRKQISITPT KADIAAVQLP AGVWHEDEFY GKSEIYRKRL NVPVTVNQAA AGATLTITYQ 130 140 150 160 170 180 GCADAGFCYP PETKTVPLSE VAAAIDATPT PAVTQTGETS KPAAQLPFSA LWALLIGIGI 190 200 210 220 230 240 AFTPCVLPMY PLISGIVLGG RQRLSTGRAL LLAFIYVQGM ALTYTALGLV VAAAGLQFQA 250 260 270 280 290 300 ALQHPYVLIG LAIVFTLLAL SMFGLFTLQL PSSLQTRLTL MSNRQQGGSP GGVFVMGAIA 310 320 330 340 350 360 GLICSPCTTA PLSAILLYIA QSGNMWLGGG TLYLYALGMG LPLMLVTVFG NRLLPKSGPW 370 380 390 400 410 420 MAHVKTAFGF VILALPVFLL ERIIGEAWGL RLWSLLGVAF FGWAFITSLQ ARRAWMRIVQ 430 440 450 460 470 480 IILLAAALIS VRPLQDWAFG SPSAQAPAHL NFTAISTVDE LNQALAQAKG KPVMLDFYAD 490 500 510 520 530 540 WCVACKEFEK YTFSDPRVQQ VLGDTVLLQA NVTANNAQDV ALLKHLQVLG LPTILFFDAQ 550 560 GQEQPQARVT GFMDAATFSA HLHDRQP

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627283 Genomic DNA. Translation: CAD06802.1. AE014613 Genomic DNA. Translation: AAO71825.1.
RefSeq	NP_458761.1. NP_807965.1.
3D structure databases
HSSP	HSSP built from PDB template 1L6P based on UniProtKB P36655.
SMR	Q8Z1A8. Positions 23-143, 449-567.
ModBase	Search...
Genome annotation databases
GeneID	1067057. 1250883.
GenomeReviews	Gene locus t4374 in contig AE014613_GR. Gene locus STY4682 in contig AL513382_GR.
KEGG	stt:t4374. sty:STY4682.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Z1A8.
OMA	Q8Z1A8. TITHILW.
Enzyme and pathway databases
BioCyc	SENT209261:T4374-MON. SENT220341:STY4682-MON.
BRENDA	1.8.1.8. 3716.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR015467. Thioredoxin_core. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR10438. Trx. 1 hit.
Pfam	PF02683. DsbD. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_SALTI

Accession

Primary (citable) accession number: Q8Z1A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)