Q8Z068 (SYM_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	all0233

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01228
Subunit structure	Monomer By similarity. HAMAP MF_01228
Subcellular location	Cytoplasm HAMAP MF_01228.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Methionine--tRNA ligase HAMAP MF_01228		PRO_0000139205
Regions
	Motif	18 – 28	11	"HIGH" region HAMAP MF_01228
	Motif	307 – 311	5	"KMSKS" region HAMAP MF_01228
Sites
	Metal binding	133	1	Zinc By similarity
	Metal binding	136	1	Zinc By similarity
	Metal binding	151	1	Zinc By similarity
	Metal binding	154	1	Zinc By similarity
	Binding site	310	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z068 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 247BEF61447BE6A7	FASTA	530	60,602
	10 20 30 40 50 60 MNLVNKAEKT FALTTPLYYV NDVPHIGSAY TTMAADAVAR FQKLLGRDVL LITGTDEHGQ 70 80 90 100 110 120 KIQRSAESLG KAPQEFCDEI VPSFMSLWRL LNIQYDRFSR TTAVRHKAIV DEFFARVWEA 130 140 150 160 170 180 GDIYQGQQKG WYCVSCEEFK EERELLEGNR CPIHVNKEVE WRDEQNYFFR LSKYQTQLEE 190 200 210 220 230 240 FYQSHPDFIQ PESRRNEVLN FVSQGLQDFS ISRVNLDWGF PVPNDPKHTL YVWFDALLAY 250 260 270 280 290 300 VTALLDPEDE PTLENALGKW WPINLHLIGK DILRFHAVYW PAMLLSAGLP LPDRVFGHGF 310 320 330 340 350 360 LTKDGQKMGK SLGNTVDPVG LVQQYGSDAV RYYFLKEIEF GKDGDFNEVR FIHVLNADLA 370 380 390 400 410 420 NDLGNLLNRT LNMVKKYCAN YALSITNEDI PAENTLKALG VDLGAKVKQA YEVLAFNQAC 430 440 450 460 470 480 GVVLSLVQAS NKFIDDQAPW SLYKQERQQE VETVLYTVLE SVRLAAYLLS PVIPNISSNI 490 500 510 520 530 YQQLGFGINF NEQTDITPFA IHAQWGLLSN KQQLGQPQPI FKRIEQTKNV

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB77757.1.
PIR	AI1835.
RefSeq	NP_484277.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8Z068.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8Z068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1103830.
GenomeReviews	Gene locus all0233 in contig BA000019_GR.
KEGG	ana:all0233.
NMPDR	fig|103690.1.peg.544.
PATRIC	22770193. VBINosSp37423_0589.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HBG721667.
OMA	FDWGIKV.
PhylomeDB	Q8Z068.
ProtClustDB	PRK11893.
Enzyme and pathway databases
BioCyc	NSP103690:ALL0233-MONOMER.
Family and domain databases
HAMAP	MF_01228. Met_tRNA_synth_type2. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_NOSS1

Accession

Primary (citable) accession number: Q8Z068

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program