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Q8Z064 (AMPA_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase

EC=3.4.11.1
Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase
Gene names
Name:pepA
Ordered Locus Names:alr0237
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloexopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Probable cytosol aminopeptidase HAMAP-Rule MF_00181
PRO_0000165716

Sites

Active site2721 Potential
Active site3471 Potential
Metal binding2601Manganese 2 By similarity
Metal binding2651Manganese 1 By similarity
Metal binding2651Manganese 2 By similarity
Metal binding2841Manganese 2 By similarity
Metal binding3431Manganese 1 By similarity
Metal binding3451Manganese 1 By similarity
Metal binding3451Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Z064 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 4A5BE617F48ACEEE

FASTA49151,918
        10         20         30         40         50         60 
MAIQLSDKPL LEWAGDTLAI ALFEDAVELT GELGSLDEKF AGILKELITE EEFTGKANST 

        70         80         90        100        110        120 
VFTRVSSNIP VRKIILVGLG KTEAFKIETL RRAAAAVGKV GKKQKSKVIG LSFPLWNNDP 

       130        140        150        160        170        180 
TASAQAIAEG LQLALYQDNR FKSDPEDKGS QVETVELLGF AGQEAAINRA NQIVSGVILA 

       190        200        210        220        230        240 
RQLVAAPANS VTPITMAETA QQIAQDYGLQ IEILEQEDCE KLGMGAFLGV ALASDLPPKF 

       250        260        270        280        290        300 
IHLTYKPEGT PKRKLAIVGK GLTFDSGGLN IKGAGSGIET MKIDMGGAAA TLGAAKAIAQ 

       310        320        330        340        350        360 
IKPNVEVHFI SAVTENMISG KAMHPGDILT ASNGKTIEVN NTDAEGRLTL ADALVYTDKL 

       370        380        390        400        410        420 
GLDAIVDLAT LTGANVIALG DDIAGLYTPD DALAGQLEQA ASESGEKIWR MPLEEKYFEG 

       430        440        450        460        470        480 
LKSGIADMKN TGPRPGGSIT AALFLKQFVK DTAWAHLDIA GPVWADKENG YNGPGATGYG 

       490 
VRLLVDWVLS E 

« Hide

References

[1]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000019 Genomic DNA. Translation: BAB77761.1.
PIRAE1836.
RefSeqNP_484281.1. NC_003272.1.

3D structure databases

ProteinModelPortalQ8Z064.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.alr0237.

Protein family/group databases

MEROPSM17.002.

Proteomic databases

PRIDEQ8Z064.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB77761; BAB77761; BAB77761.
GeneID1103834.
KEGGana:alr0237.
PATRIC22770201. VBINosSp37423_0593.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0260.
HOGENOMHOG000243129.
KOK01255.
OMAKYDWAHL.
OrthoDBEOG6FV8B3.
ProtClustDBPRK00913.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERPTHR11963:SF3. PTHR11963:SF3. 1 hit.
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPA_NOSS1
AccessionPrimary (citable) accession number: Q8Z064
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries