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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi260 – 2601Manganese 2UniRule annotation
Metal bindingi265 – 2651Manganese 1UniRule annotation
Metal bindingi265 – 2651Manganese 2UniRule annotation
Active sitei272 – 2721UniRule annotation
Metal bindingi284 – 2841Manganese 2UniRule annotation
Metal bindingi343 – 3431Manganese 1UniRule annotation
Metal bindingi345 – 3451Manganese 1UniRule annotation
Metal bindingi345 – 3451Manganese 2UniRule annotation
Active sitei347 – 3471UniRule annotation

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

MEROPSiM17.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:alr0237
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Probable cytosol aminopeptidasePRO_0000165716Add
BLAST

Proteomic databases

PRIDEiQ8Z064.

Interactioni

Protein-protein interaction databases

STRINGi103690.alr0237.

Structurei

3D structure databases

ProteinModelPortaliQ8Z064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiTAWRMPL.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Z064-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIQLSDKPL LEWAGDTLAI ALFEDAVELT GELGSLDEKF AGILKELITE
60 70 80 90 100
EEFTGKANST VFTRVSSNIP VRKIILVGLG KTEAFKIETL RRAAAAVGKV
110 120 130 140 150
GKKQKSKVIG LSFPLWNNDP TASAQAIAEG LQLALYQDNR FKSDPEDKGS
160 170 180 190 200
QVETVELLGF AGQEAAINRA NQIVSGVILA RQLVAAPANS VTPITMAETA
210 220 230 240 250
QQIAQDYGLQ IEILEQEDCE KLGMGAFLGV ALASDLPPKF IHLTYKPEGT
260 270 280 290 300
PKRKLAIVGK GLTFDSGGLN IKGAGSGIET MKIDMGGAAA TLGAAKAIAQ
310 320 330 340 350
IKPNVEVHFI SAVTENMISG KAMHPGDILT ASNGKTIEVN NTDAEGRLTL
360 370 380 390 400
ADALVYTDKL GLDAIVDLAT LTGANVIALG DDIAGLYTPD DALAGQLEQA
410 420 430 440 450
ASESGEKIWR MPLEEKYFEG LKSGIADMKN TGPRPGGSIT AALFLKQFVK
460 470 480 490
DTAWAHLDIA GPVWADKENG YNGPGATGYG VRLLVDWVLS E
Length:491
Mass (Da):51,918
Last modified:March 1, 2002 - v1
Checksum:i4A5BE617F48ACEEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB77761.1.
PIRiAE1836.
RefSeqiNP_484281.1. NC_003272.1.
WP_010994414.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB77761; BAB77761; BAB77761.
GeneIDi1103834.
KEGGiana:alr0237.
PATRICi22770201. VBINosSp37423_0593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB77761.1.
PIRiAE1836.
RefSeqiNP_484281.1. NC_003272.1.
WP_010994414.1. NC_003272.1.

3D structure databases

ProteinModelPortaliQ8Z064.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.alr0237.

Protein family/group databases

MEROPSiM17.A03.

Proteomic databases

PRIDEiQ8Z064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB77761; BAB77761; BAB77761.
GeneIDi1103834.
KEGGiana:alr0237.
PATRICi22770201. VBINosSp37423_0593.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiTAWRMPL.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiAMPA_NOSS1
AccessioniPrimary (citable) accession number: Q8Z064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.