Reviewed, UniProtKB/Swiss-Prot Q8Z064 (AMPA_ANASP)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	alr0237

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165716
Sites
	Active site	272	1	Potential
	Active site	347	1	Potential
	Metal binding	260	1	Manganese 2 By similarity
	Metal binding	265	1	Manganese 1 By similarity
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	284	1	Manganese 2 By similarity
	Metal binding	343	1	Manganese 1 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	345	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Z064-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4A5BE617F48ACEEE	FASTA	491	51,918
	10 20 30 40 50 60 MAIQLSDKPL LEWAGDTLAI ALFEDAVELT GELGSLDEKF AGILKELITE EEFTGKANST 70 80 90 100 110 120 VFTRVSSNIP VRKIILVGLG KTEAFKIETL RRAAAAVGKV GKKQKSKVIG LSFPLWNNDP 130 140 150 160 170 180 TASAQAIAEG LQLALYQDNR FKSDPEDKGS QVETVELLGF AGQEAAINRA NQIVSGVILA 190 200 210 220 230 240 RQLVAAPANS VTPITMAETA QQIAQDYGLQ IEILEQEDCE KLGMGAFLGV ALASDLPPKF 250 260 270 280 290 300 IHLTYKPEGT PKRKLAIVGK GLTFDSGGLN IKGAGSGIET MKIDMGGAAA TLGAAKAIAQ 310 320 330 340 350 360 IKPNVEVHFI SAVTENMISG KAMHPGDILT ASNGKTIEVN NTDAEGRLTL ADALVYTDKL 370 380 390 400 410 420 GLDAIVDLAT LTGANVIALG DDIAGLYTPD DALAGQLEQA ASESGEKIWR MPLEEKYFEG 430 440 450 460 470 480 LKSGIADMKN TGPRPGGSIT AALFLKQFVK DTAWAHLDIA GPVWADKENG YNGPGATGYG 490 VRLLVDWVLS E

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB77761.1.
PIR	AE1836.
RefSeq	NP_484281.1.
3D structure databases
SMR	Q8Z064. Positions 16-490.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8Z064.
Protein family/group databases
MEROPS	M17.002.
Genome annotation databases
GeneID	1103834.
GenomeReviews	Gene locus alr0237 in contig BA000019_GR.
KEGG	ana:alr0237.
NMPDR	fig|103690.1.peg.548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	LGHHNSG.
Enzyme and pathway databases
BioCyc	NSP103690:ALR0237-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ANASP

Accession

Primary (citable) accession number: Q8Z064

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)