Reviewed, UniProtKB/Swiss-Prot Q8YZA1 (DNAB_ANASP)

Names and origin

Protein names

Recommended name:
    Replicative DNA helicase
    EC=3.6.1.-
Cleaved into the following chain:
    1- Recommended name:
            Endonuclease PI-AspHIP
              EC=3.1.-.-
        Alternative name(s):
            Asp dnaB intein

Gene names

Name:	dnaB
Ordered Locus Names:	all0578

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	879 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity. The intein is an endonuclease Potential.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Intron homing
Cellular component	Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Replicative DNA helicase, 1st part Potential		PRO_0000013270
	Chain	389 – 817	429	Endonuclease PI-AspHIP		PRO_0000013271
	Chain	818 – 879	62	Replicative DNA helicase, 2nd part Potential		PRO_0000013272
Regions
	Domain	184 – 451	268	SF4 helicase; first part
	Domain	502 – 651	150	DOD-type homing endonuclease
	Domain	614 – 878	265	SF4 helicase; second part
	Nucleotide binding	215 – 222	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YZA1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E42CB186E2B26D11	FASTA	879	98,008
	10 20 30 40 50 60 MAEELSFQGD GSNRLPPQNI EAEEAILGGI LLDPEAIGRV SEVLITEAFY ISAHRDIYQA 70 80 90 100 110 120 ALRLHAQGKP TDLLSVTSWL TDNELLTRIG GRNKLATLVD RTVSAVNIDA LAGLVMEKYL 130 140 150 160 170 180 RRQLIKAGNE IVHLGYETEK ELPIVLDQAE QKVFGVTQER PQSGLVHIAD TLINNFQEIE 190 200 210 220 230 240 ERNQGIALPG IPCGFYDLDA MTSGFQRSDL IIVAGRPSMG KTAFCLNLAN NIAATMKLPV 250 260 270 280 290 300 AVFSLEMSKE QLVQRLLASE AQIESGYLRS GRLSQTQWEP LSRAISMLSE MPIFIDDTPN 310 320 330 340 350 360 ITVTQMRSQA RRLQAEQGVE LGLIVIDYLQ LMEGGGDNRV QELSKITRSL KGLARELSVP 370 380 390 400 410 420 VIALSQLSRG VEARTNKRPM LSDLRESGCL TGDSLVTLAP GGLQIPIREL VGKSGFAIWA 430 440 450 460 470 480 LNQATLKLEK AIVSNAFSTG VKPVFTLTTR KGCKIRATAN HKFLTIHGWR RLDELSIRQH 490 500 510 520 530 540 LCLPRHIPSF DKQTMTYAEV ALLGHLIGDG CTLPRHAIQY TTREIDLAEN VFFLAKEVFG 550 560 570 580 590 600 DAITPRISPE RSWYQVYLSA TQRLTRNLRN PVAKWLDSLG VFGLRSYEKF VPQELFSQPI 610 620 630 640 650 660 ELISCFLRHL WSTDGSIKLV GGKKPRPIAY YATSSYRLAV DVQTLLLKLG INATLKLVPQ 670 680 690 700 710 720 VGKGRNQYHV KITGKPDLDL FIKKVGAVGE YKLNSLQQIF EYLENCIHNT NRDIIPKDVW 730 740 750 760 770 780 KKIVVPAMQS VQLTTRELHS NIGTSYCGLA LYKANLSRER ALKVAEVVQS SELLTLAKSD 790 800 810 820 830 840 VYWDEIVSIE YSGEEEVFDL TVPGLHNFIA NNIVVHNSIE QDADLVIMLY RDDYYNSDTP 850 860 870 DRGLAEVIVA KHRNGPTGTV KLLFDPQFTK FKNLARSGY

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB72536.1.
PIR	AI1878.
RefSeq	NP_484622.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YZA1.
Genome annotation databases
GeneID	1104174.
GenomeReviews	Gene locus all0578 in contig BA000019_GR.
KEGG	ana:all0578.
NMPDR	fig|103690.1.peg.889.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YZA1.
OMA	WDEIVSI
Enzyme and pathway databases
BioCyc	NSP103690:ALL0578-MON.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR016136. DNA_helicase_N/primase_C. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. [Graphical view]
Gene3D	G3DSA:1.10.860.10. DnaB_N. 1 hit.
Pfam	PF00772. DnaB. 1 hit. PF03796. DnaB_C. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00665. DnaB. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS51199. SF4_HELICASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_ANASP

Accession

Primary (citable) accession number: Q8YZA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	March 1, 2002
Last modified:	November 24, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)