Reviewed, UniProtKB/Swiss-Prot Q8YYX9 (GLGB_ANASP)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	all0713

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188672
Sites
	Active site	329	1	By similarity
	Active site	364	1	By similarity
	Active site	369	1	By similarity
	Active site	432	1	By similarity
	Active site	434	1	By similarity
	Active site	487	1	By similarity
	Active site	554	1	By similarity
	Active site	555	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YYX9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: A801D1F3B141C772	FASTA	764	88,808
	10 20 30 40 50 60 MSMTTIAPEQ VNRIVWNQHH DPFEILGSHP IEQNGKTVWV VRAYLPNASA AWVVLPEQRQ 70 80 90 100 110 120 EYPMQTVHDP HFFECIIETS ELSNYQLKTK EGEHERVSYD PYAFRSPRLT DFDLHLFAEG 130 140 150 160 170 180 NHHRIYEKLG AHFTEVGGVK GVYFAVWAPN ARNVSVLGDF NLWDGRKHQM RKGATGVWEL 190 200 210 220 230 240 FIPEIGVGEH YKYEIKNFAG HIYEKSDPFG FQQEPRPKTA SIVSNLNSYN WSDEDWLEQR 250 260 270 280 290 300 RHTDPLTQPI SVYEVHLGSW LHAASAEPAQ LPNGETEPVV IASELNPGAR FLTYRELASR 310 320 330 340 350 360 LIPYVKELGY THIELLPIAE HPFDGSWGYQ VTGYYAPTSR FGTPEDFMYF VDQCHQNNIG 370 380 390 400 410 420 VLVDWVPGHF PKDGHGLAFF DGTHLYEHAD PRKGEHKEWG TLVFNYSRNE VRNFLVANAL 430 440 450 460 470 480 FWFDKYHIDG IRVDAVASML YLDYCRKEGE WLPNQYGGRE NLEAADFLRQ VNHLLFSYFP 490 500 510 520 530 540 GVLSIAEEST DWPMVSWPTY TGGLGFNLKW NMGWMHDMLD YFSMDPWFRQ FHQNNITFSM 550 560 570 580 590 600 WYNHSENFML ALSHDEVVHG KSNIIGKMPG DKWQKLANVR CLFAYMFAHP GKKTMFMSME 610 620 630 640 650 660 FGQWSEWNVW ADLEWPLLQF EPHQQLKKFF TELNKLYRSE PALYTLDFAR EGFDWIDCSD 670 680 690 700 710 720 NRHSVVSFIR REKDTENFVV VICNFTPQPH SHYRIGVPEK GFYTELFNSD ARQYGGSNMG 730 740 750 760 NLGGKWTDDW SMHNRPYSLD LCLPPLGVLI LKMDKEKTAK ALGS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB72670.1.
PIR	AG1895.
RefSeq	NP_484756.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YYX9.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1104308.
GenomeReviews	Gene locus all0713 in contig BA000019_GR.
KEGG	ana:all0713.
NMPDR	fig|103690.1.peg.1023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YYX9.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	NSP103690:ALL0713-MON.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 2 hits. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_ANASP

Accession

Primary (citable) accession number: Q8YYX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)