Reviewed, UniProtKB/Swiss-Prot Q8YY71 (DDL_ANASP)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	all0980

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	D-alanine--D-alanine ligase		PRO_0000177779
Regions
	Domain	145 – 354	210	ATP-grasp
	Nucleotide binding	181 – 236	56	ATP By similarity
Sites
	Metal binding	307	1	Magnesium or manganese 1 By similarity
	Metal binding	321	1	Magnesium or manganese 1 By similarity
	Metal binding	321	1	Magnesium or manganese 2 By similarity
	Metal binding	323	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YY71-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 17E8ECC3BA36BF7F	FASTA	364	39,427
	10 20 30 40 50 60 MRVGLLFGGR SGEHEVSISS ARAIASALSA GENASKYEIL PFYIHKDGRW LAGEAPQQVL 70 80 90 100 110 120 KSGAPLLESS NSSPAENNLV NSQQQTLERW QSPSQVAEVD VWFPILHGPN GEDGTIQGLL 130 140 150 160 170 180 TLMQTPFVGS GVLGSALGMD KIAMKMAFEQ AGLPQVKYKA VTRAQIWSNP CVFPKLCDEI 190 200 210 220 230 240 EASLGYPCFV KPANLGSSVG ISKVRSRQEL EDALDNAANY DRRIIIEAGV AAREVECAVL 250 260 270 280 290 300 GNDQPQASTV GEITFNSDFY DYETKYTAGK ADLLIPAIIP DDISRQIQNM ALQAFAAVDA 310 320 330 340 350 360 AGLARVDFFY VEATGEVLIN EINTLPGFTA TSMYPQLWAH SGIPFPELVD KLVQLAIERH NPSH

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB72937.1.
PIR	AI1928.
RefSeq	NP_485023.1.
3D structure databases
HSSP	HSSP built from PDB template 1EHI based on UniProtKB P71454.
ModBase	Search...
Genome annotation databases
GeneID	1104574.
GenomeReviews	Gene locus all0980 in contig BA000019_GR.
KEGG	ana:all0980.
NMPDR	fig|103690.1.peg.1290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YY71.
Enzyme and pathway databases
BioCyc	NSP103690:ALL0980-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ANASP

Accession

Primary (citable) accession number: Q8YY71

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	March 1, 2002
Last modified:	November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)