Reviewed, UniProtKB/Swiss-Prot Q8YXY3 (SYI_ANASP)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	alr1073

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	960 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 960	960	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_0000098340
Regions
	Motif	60 – 70	11	"HIGH" region HAMAP MF_02002
	Motif	614 – 618	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	929	1	Zinc By similarity
	Metal binding	932	1	Zinc By similarity
	Metal binding	949	1	Zinc By similarity
	Metal binding	952	1	Zinc By similarity
	Binding site	573	1	Aminoacyl-adenylate By similarity
	Binding site	617	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YXY3-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: B22328C27781C30A	FASTA	960	108,394
	10 20 30 40 50 60 MTETGSYKDT VNLPKTNFDM RANAIKREPE IQKFWEENKI FESLSQNNPG ELFILHDGPP 70 80 90 100 110 120 YANGSLHIGH ALNKILKDII NRYQLLQGRK VRYVPGWDCH GLPIELKVLQ NLKSAERQNL 130 140 150 160 170 180 TPLQLRQKAK EFALATVDNQ RQNFKRYGVW GDWDNPYLTL KPEYEAAQIG VFGQMVLKGY 190 200 210 220 230 240 IYRGLKPVHW SPSSKTALAE AELEYPEGHT SRSIYAAFPV TSFAEAAKPL LGEYLPDLGV 250 260 270 280 290 300 AIWTTTPWTI PGNLAVAVNG DLNYSLVEVS RIGAETQSNF KYLIVAADLV ERLAATISAQ 310 320 330 340 350 360 LTVKATFKGK ELEHTTYRHP LFDRESPVVV GGDYITTESG TGLVHTAPGH GQEDYVVGLR 370 380 390 400 410 420 YGLPILAPVD DNGDFTQEAG QFAGLNVLGE GNQAVIDALT AAGSLLKEEA YAHKYPYDWR 430 440 450 460 470 480 TKKPTIFRAT EQWFASVEGF RDEALKAIAA VKWIPAQGEN RITPMVAERS DWCISRQRSW 490 500 510 520 530 540 GVPIPVFYDE ETGEPLLNEE TINYVQAIIA EKGSDAWWEL SVAELLPESY RNNGRSYRRG 550 560 570 580 590 600 TDTMDVWFDS GSSWASVVKQ RPELRYPADM YLEGSDQHRG WFQSSLLTSV SVNGIAPYKT 610 620 630 640 650 660 VLTHGFVLDE QGRKMSKSEG NVVDPAIIIN GGKDQKKEPP YGADVMRLWA SSVDYTGDVR 670 680 690 700 710 720 LGGNIIKQLN DVRGKIRNTA RFLLGSLYDF DPEKNAVQFE EMPQLDKYML HRIREVFEEV 730 740 750 760 770 780 TEAFESFQFF RFFQTVQNFC VVDLSNFYLD VAKDRLYISA PDAFRRRSCQ TVIHIALENL 790 800 810 820 830 840 ARAIAPVLCH TAEDIWQYLP YKTPYKSVFE AGWVQVEEKW HNPELAEFWQ QLRQLRTDVN 850 860 870 880 890 900 KVLEQARVEK MIGSSLEAKA LIYVKDANSR KAIATLNPEV GNGVDELRYL FLTSQVELLD 910 920 930 940 950 960 SADKLQDGKY TSQSDSWGIG VVNAEGQKCD RCWNYSTHVG ESAEHPLLCE RCVPALAGEF

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73030.1.
PIR	AF1940.
RefSeq	NP_485116.1.
3D structure databases
HSSP	HSSP built from PDB template 1FFY based on UniProtKB P41972.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YXY3.
Genome annotation databases
GeneID	1104667.
GenomeReviews	Gene locus alr1073 in contig BA000019_GR.
KEGG	ana:alr1073.
NMPDR	fig|103690.1.peg.1383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YXY3.
OMA	FPMRGNL.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1073-MON.
Family and domain databases
HAMAP	MF_02002. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_ANASP

Accession

Primary (citable) accession number: Q8YXY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)