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Q8YXY3

- SYI_NOSS1

UniProt

Q8YXY3 - SYI_NOSS1

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Protein
Isoleucine--tRNA ligase
Gene
ileS, alr1073
Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731Aminoacyl-adenylate By similarity
Binding sitei617 – 6171ATP By similarity
Metal bindingi929 – 9291Zinc By similarity
Metal bindingi932 – 9321Zinc By similarity
Metal bindingi949 – 9491Zinc By similarity
Metal bindingi952 – 9521Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:alr1073
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098340Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi103690.alr1073.

Structurei

3D structure databases

ProteinModelPortaliQ8YXY3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi614 – 6185"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEVMDEIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YXY3-1 [UniParc]FASTAAdd to Basket

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MTETGSYKDT VNLPKTNFDM RANAIKREPE IQKFWEENKI FESLSQNNPG    50
ELFILHDGPP YANGSLHIGH ALNKILKDII NRYQLLQGRK VRYVPGWDCH 100
GLPIELKVLQ NLKSAERQNL TPLQLRQKAK EFALATVDNQ RQNFKRYGVW 150
GDWDNPYLTL KPEYEAAQIG VFGQMVLKGY IYRGLKPVHW SPSSKTALAE 200
AELEYPEGHT SRSIYAAFPV TSFAEAAKPL LGEYLPDLGV AIWTTTPWTI 250
PGNLAVAVNG DLNYSLVEVS RIGAETQSNF KYLIVAADLV ERLAATISAQ 300
LTVKATFKGK ELEHTTYRHP LFDRESPVVV GGDYITTESG TGLVHTAPGH 350
GQEDYVVGLR YGLPILAPVD DNGDFTQEAG QFAGLNVLGE GNQAVIDALT 400
AAGSLLKEEA YAHKYPYDWR TKKPTIFRAT EQWFASVEGF RDEALKAIAA 450
VKWIPAQGEN RITPMVAERS DWCISRQRSW GVPIPVFYDE ETGEPLLNEE 500
TINYVQAIIA EKGSDAWWEL SVAELLPESY RNNGRSYRRG TDTMDVWFDS 550
GSSWASVVKQ RPELRYPADM YLEGSDQHRG WFQSSLLTSV SVNGIAPYKT 600
VLTHGFVLDE QGRKMSKSEG NVVDPAIIIN GGKDQKKEPP YGADVMRLWA 650
SSVDYTGDVR LGGNIIKQLN DVRGKIRNTA RFLLGSLYDF DPEKNAVQFE 700
EMPQLDKYML HRIREVFEEV TEAFESFQFF RFFQTVQNFC VVDLSNFYLD 750
VAKDRLYISA PDAFRRRSCQ TVIHIALENL ARAIAPVLCH TAEDIWQYLP 800
YKTPYKSVFE AGWVQVEEKW HNPELAEFWQ QLRQLRTDVN KVLEQARVEK 850
MIGSSLEAKA LIYVKDANSR KAIATLNPEV GNGVDELRYL FLTSQVELLD 900
SADKLQDGKY TSQSDSWGIG VVNAEGQKCD RCWNYSTHVG ESAEHPLLCE 950
RCVPALAGEF 960
Length:960
Mass (Da):108,394
Last modified:March 1, 2002 - v1
Checksum:iB22328C27781C30A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000019 Genomic DNA. Translation: BAB73030.1.
PIRiAF1940.
RefSeqiNP_485116.1. NC_003272.1.
WP_010995247.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB73030; BAB73030; BAB73030.
GeneIDi1104667.
KEGGiana:alr1073.
PATRICi22772058. VBINosSp37423_1513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000019 Genomic DNA. Translation: BAB73030.1 .
PIRi AF1940.
RefSeqi NP_485116.1. NC_003272.1.
WP_010995247.1. NC_003272.1.

3D structure databases

ProteinModelPortali Q8YXY3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 103690.alr1073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB73030 ; BAB73030 ; BAB73030 .
GeneIDi 1104667.
KEGGi ana:alr1073.
PATRICi 22772058. VBINosSp37423_1513.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EVMDEIT.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiSYI_NOSS1
AccessioniPrimary (citable) accession number: Q8YXY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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