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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731Aminoacyl-adenylateUniRule annotation
Binding sitei617 – 6171ATPUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation
Metal bindingi932 – 9321ZincUniRule annotation
Metal bindingi949 – 9491ZincUniRule annotation
Metal bindingi952 – 9521ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:alr1073
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 960960Isoleucine--tRNA ligasePRO_0000098340Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi103690.alr1073.

Structurei

3D structure databases

ProteinModelPortaliQ8YXY3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi614 – 6185"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YXY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETGSYKDT VNLPKTNFDM RANAIKREPE IQKFWEENKI FESLSQNNPG
60 70 80 90 100
ELFILHDGPP YANGSLHIGH ALNKILKDII NRYQLLQGRK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ NLKSAERQNL TPLQLRQKAK EFALATVDNQ RQNFKRYGVW
160 170 180 190 200
GDWDNPYLTL KPEYEAAQIG VFGQMVLKGY IYRGLKPVHW SPSSKTALAE
210 220 230 240 250
AELEYPEGHT SRSIYAAFPV TSFAEAAKPL LGEYLPDLGV AIWTTTPWTI
260 270 280 290 300
PGNLAVAVNG DLNYSLVEVS RIGAETQSNF KYLIVAADLV ERLAATISAQ
310 320 330 340 350
LTVKATFKGK ELEHTTYRHP LFDRESPVVV GGDYITTESG TGLVHTAPGH
360 370 380 390 400
GQEDYVVGLR YGLPILAPVD DNGDFTQEAG QFAGLNVLGE GNQAVIDALT
410 420 430 440 450
AAGSLLKEEA YAHKYPYDWR TKKPTIFRAT EQWFASVEGF RDEALKAIAA
460 470 480 490 500
VKWIPAQGEN RITPMVAERS DWCISRQRSW GVPIPVFYDE ETGEPLLNEE
510 520 530 540 550
TINYVQAIIA EKGSDAWWEL SVAELLPESY RNNGRSYRRG TDTMDVWFDS
560 570 580 590 600
GSSWASVVKQ RPELRYPADM YLEGSDQHRG WFQSSLLTSV SVNGIAPYKT
610 620 630 640 650
VLTHGFVLDE QGRKMSKSEG NVVDPAIIIN GGKDQKKEPP YGADVMRLWA
660 670 680 690 700
SSVDYTGDVR LGGNIIKQLN DVRGKIRNTA RFLLGSLYDF DPEKNAVQFE
710 720 730 740 750
EMPQLDKYML HRIREVFEEV TEAFESFQFF RFFQTVQNFC VVDLSNFYLD
760 770 780 790 800
VAKDRLYISA PDAFRRRSCQ TVIHIALENL ARAIAPVLCH TAEDIWQYLP
810 820 830 840 850
YKTPYKSVFE AGWVQVEEKW HNPELAEFWQ QLRQLRTDVN KVLEQARVEK
860 870 880 890 900
MIGSSLEAKA LIYVKDANSR KAIATLNPEV GNGVDELRYL FLTSQVELLD
910 920 930 940 950
SADKLQDGKY TSQSDSWGIG VVNAEGQKCD RCWNYSTHVG ESAEHPLLCE
960
RCVPALAGEF
Length:960
Mass (Da):108,394
Last modified:March 1, 2002 - v1
Checksum:iB22328C27781C30A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB73030.1.
PIRiAF1940.
RefSeqiWP_010995247.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB73030; BAB73030; BAB73030.
KEGGiana:alr1073.
PATRICi22772058. VBINosSp37423_1513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB73030.1.
PIRiAF1940.
RefSeqiWP_010995247.1. NC_003272.1.

3D structure databases

ProteinModelPortaliQ8YXY3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.alr1073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB73030; BAB73030; BAB73030.
KEGGiana:alr1073.
PATRICi22772058. VBINosSp37423_1513.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiSYI_NOSS1
AccessioniPrimary (citable) accession number: Q8YXY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.