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Q8YXY3

- SYI_NOSS1

UniProt

Q8YXY3 - SYI_NOSS1

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei573 – 5731Aminoacyl-adenylateUniRule annotation
    Binding sitei617 – 6171ATPUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation
    Metal bindingi932 – 9321ZincUniRule annotation
    Metal bindingi949 – 9491ZincUniRule annotation
    Metal bindingi952 – 9521ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:alr1073
    OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
    Taxonomic identifieri103690 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000002483: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 960960Isoleucine--tRNA ligasePRO_0000098340Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi103690.alr1073.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YXY3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi614 – 6185"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiEVMDEIT.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YXY3-1 [UniParc]FASTAAdd to Basket

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    MTETGSYKDT VNLPKTNFDM RANAIKREPE IQKFWEENKI FESLSQNNPG    50
    ELFILHDGPP YANGSLHIGH ALNKILKDII NRYQLLQGRK VRYVPGWDCH 100
    GLPIELKVLQ NLKSAERQNL TPLQLRQKAK EFALATVDNQ RQNFKRYGVW 150
    GDWDNPYLTL KPEYEAAQIG VFGQMVLKGY IYRGLKPVHW SPSSKTALAE 200
    AELEYPEGHT SRSIYAAFPV TSFAEAAKPL LGEYLPDLGV AIWTTTPWTI 250
    PGNLAVAVNG DLNYSLVEVS RIGAETQSNF KYLIVAADLV ERLAATISAQ 300
    LTVKATFKGK ELEHTTYRHP LFDRESPVVV GGDYITTESG TGLVHTAPGH 350
    GQEDYVVGLR YGLPILAPVD DNGDFTQEAG QFAGLNVLGE GNQAVIDALT 400
    AAGSLLKEEA YAHKYPYDWR TKKPTIFRAT EQWFASVEGF RDEALKAIAA 450
    VKWIPAQGEN RITPMVAERS DWCISRQRSW GVPIPVFYDE ETGEPLLNEE 500
    TINYVQAIIA EKGSDAWWEL SVAELLPESY RNNGRSYRRG TDTMDVWFDS 550
    GSSWASVVKQ RPELRYPADM YLEGSDQHRG WFQSSLLTSV SVNGIAPYKT 600
    VLTHGFVLDE QGRKMSKSEG NVVDPAIIIN GGKDQKKEPP YGADVMRLWA 650
    SSVDYTGDVR LGGNIIKQLN DVRGKIRNTA RFLLGSLYDF DPEKNAVQFE 700
    EMPQLDKYML HRIREVFEEV TEAFESFQFF RFFQTVQNFC VVDLSNFYLD 750
    VAKDRLYISA PDAFRRRSCQ TVIHIALENL ARAIAPVLCH TAEDIWQYLP 800
    YKTPYKSVFE AGWVQVEEKW HNPELAEFWQ QLRQLRTDVN KVLEQARVEK 850
    MIGSSLEAKA LIYVKDANSR KAIATLNPEV GNGVDELRYL FLTSQVELLD 900
    SADKLQDGKY TSQSDSWGIG VVNAEGQKCD RCWNYSTHVG ESAEHPLLCE 950
    RCVPALAGEF 960
    Length:960
    Mass (Da):108,394
    Last modified:March 1, 2002 - v1
    Checksum:iB22328C27781C30A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000019 Genomic DNA. Translation: BAB73030.1.
    PIRiAF1940.
    RefSeqiNP_485116.1. NC_003272.1.
    WP_010995247.1. NC_003272.1.

    Genome annotation databases

    EnsemblBacteriaiBAB73030; BAB73030; BAB73030.
    GeneIDi1104667.
    KEGGiana:alr1073.
    PATRICi22772058. VBINosSp37423_1513.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000019 Genomic DNA. Translation: BAB73030.1 .
    PIRi AF1940.
    RefSeqi NP_485116.1. NC_003272.1.
    WP_010995247.1. NC_003272.1.

    3D structure databases

    ProteinModelPortali Q8YXY3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 103690.alr1073.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB73030 ; BAB73030 ; BAB73030 .
    GeneIDi 1104667.
    KEGGi ana:alr1073.
    PATRICi 22772058. VBINosSp37423_1513.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi EVMDEIT.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7120 / UTEX 2576.

    Entry informationi

    Entry nameiSYI_NOSS1
    AccessioniPrimary (citable) accession number: Q8YXY3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3