Q8YXW5 (SYC_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	all1092

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159339
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	271 – 275	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Metal binding	243	1	Zinc By similarity
	Binding site	274	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YXW5 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E93ED2331C54C1B3	FASTA	486	55,140
	10 20 30 40 50 60 MTLTIYNTLT RRQEPLETVE PGKVKMYCCG VTVYDYCHLG HARSYIVWDT IRRYLIWRGF 70 80 90 100 110 120 GVKYIQNFTD IDDKILNRAK EQGLTMAEVS NRFIDAYFAD IRRLNVLDAD EYPRVTEHIP 130 140 150 160 170 180 EIHQLIQILE EKGLAYAVGG DVYYRVERFP SYGKLSGREL EQMQAGASGR VDAEDSEPKK 190 200 210 220 230 240 QHPFDFALWK AAKPGEPAWD SPWGAGRPGW HIECSAMIRS KLGATIDIHG GGGDLIFPHH 250 260 270 280 290 300 ENEIAQSEAA MNQPLARYWT HNGMVMVNGQ KMSKSLGNFI TIRELLDGVG SWKEDPVNPM 310 320 330 340 350 360 AVRLFVLQAH YRKPLDFTEE AIATAENSWK TLKEGLLFGY QYGEKLGWGQ ESAIIPELAT 370 380 390 400 410 420 RFQELGDDDF NFSGGLAVLF ELAKELRREG NILVHEGTTK TPSDELQRQW NTLVTLAKIL 430 440 450 460 470 480 GLEAKLDDET QTQAGLSDTD IEALIEQRQA ARKNKNFSES DRIRNELQAQ GVTLIDSPQG TRWHRS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB73049.1.
PIR	AI1942.
RefSeq	NP_485135.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q8YXW5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YXW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1104686.
GenomeReviews	Gene locus all1092 in contig BA000019_GR.
KEGG	ana:all1092.
NMPDR	fig|103690.1.peg.1402.
PATRIC	22772100. VBINosSp37423_1534.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	DFDALNM.
PhylomeDB	Q8YXW5.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	NSP103690:ALL1092-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_NOSS1

Accession

Primary (citable) accession number: Q8YXW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program