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Protein

UvrABC system protein B

Gene

uvrB

Organism
Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:all1132
OrganismiNostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000002483 Componenti: Chromosome

Subcellular locationi

Q8YXS9:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001383771 – 665UvrABC system protein BAdd BLAST665

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi103690.all1132.

Structurei

3D structure databases

ProteinModelPortaliQ8YXS9.
SMRiQ8YXS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 412Helicase ATP-bindingUniRule annotationAdd BLAST388
Domaini429 – 583Helicase C-terminalUniRule annotationAdd BLAST155
Domaini626 – 661UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi91 – 114Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiRYMHSEI.
OrthoDBiPOG091H02LK.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YXS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFGLQAPF SPTGDQPSAI AQLVASIEGG NRYQTLLGAT GTGKTFSIAA
60 70 80 90 100
VIEKIGRPTL VLAHNKTLAA QLCNELREFF PNNAVEYFVS YYDYYQPEAY
110 120 130 140 150
IPVTDTYIEK TAAINDEIDM LRHSATRSLF ERRDVIVVAS ISCIYGLGIP
160 170 180 190 200
AEYLKAAIPL QIGMEVNQRE ILRDLASVQY SRNDVEMGRG RFRVRGDVLE
210 220 230 240 250
IGPAYEDRII RVEFFGDEID AIRYIDPVTG EIINSLQAVN IYPARHFVTP
260 270 280 290 300
EERLEVACED IAYELKQRKA ELEETGKLVE AQRIDQRTRY DLEMLREVGY
310 320 330 340 350
CNGVENYSRH LAGRQAGEPP ESLIDYFPKD WLLVIDESHV TVPQIRGMYN
360 370 380 390 400
GDQARKKVLI EHGFRLPSAA DNRPLKAEEF WQKVNQCIFV SATPGNWELE
410 420 430 440 450
ISENRIVEQV IRPTGVIDPE ISVRPTEGQI DDLLGEIKDR IDLHERVLIT
460 470 480 490 500
TLTKRMAEDL TEYLQEHGVK VRYLHSEINS IQRIEILQDL RQGSFDVLVG
510 520 530 540 550
VNLLREGLDL PEVSLVAIMD ADKEGFLRAE RSLIQTIGRA ARHIRGQAIL
560 570 580 590 600
YADNMTDSMI KAIEETDRRR GIQVAYNKLH GITPQPIVKK SSNAILSFLE
610 620 630 640 650
VSRRLNATDL KVVEEHIDEL PLEEIPNLID KLEAQMKEAS KKLEFEEAAK
660
LRDRIKQLRD KLVGR
Length:665
Mass (Da):75,587
Last modified:March 1, 2002 - v1
Checksum:i313B2AE5D836F690
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB73089.1.
PIRiAI1947.
RefSeqiWP_010995305.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB73089; BAB73089; BAB73089.
KEGGiana:all1132.

Similar proteinsi

Entry informationi

Entry nameiUVRB_NOSS1
AccessioniPrimary (citable) accession number: Q8YXS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families