Reviewed, UniProtKB/Swiss-Prot Q8YXQ7 (CARA_ANASP)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	alr1155

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112244
Regions
	Domain	196 – 382	187	Glutamine amidotransferase type-1
	Region	1 – 192	192	CPSase HAMAP MF_01209
Sites
	Active site	272	1	Nucleophile By similarity
	Active site	355	1	By similarity
	Active site	357	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YXQ7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 062A038D8F4B706E	FASTA	388	42,257
	10 20 30 40 50 60 MPLSDAMPAL LVLADGTAYR GWSFGATGTT IGEVVFNTGM TGYQEVLTDP SYRGQIVVFT 70 80 90 100 110 120 YPELGNTGVN PEDEESARPQ VRGAIARNIC HKPSNWRSTQ SLPDYLKQHH IPGIFGIDTR 130 140 150 160 170 180 ALTRKIRMYG AMNGGISTEI LDEAELLEQV QAAPNMAGLN LVREVTTSTV YEWSDPTTAV 190 200 210 220 230 240 WEFNPDGTVK NGESLTVVAL DFGVKRNILR RLASYGCRVI VVPADTSPEE ILKYNPDGIF 250 260 270 280 290 300 LSNGPGDPSA VTEGITTAKA LLESEKPIFG ICMGHQILGH ALGAETFKLK FGHRGLNQPA 310 320 330 340 350 360 GLTQRVEITS QNHSFAIDPD SLPEAVVEIS HLNLNDRTVA GLRHKSLPIF SVQYHPEASP 370 380 GPHDADYLFA QFVHQMRTTK QATTAEVS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73112.1.
PIR	AH1950.
RefSeq	NP_485198.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YXQ7.
Genome annotation databases
GeneID	1104749.
GenomeReviews	Gene locus alr1155 in contig BA000019_GR.
KEGG	ana:alr1155.
NMPDR	fig|103690.1.peg.1465.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YXQ7.
OMA	LFDGSNC.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1155-MON.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_ANASP

Accession

Primary (citable) accession number: Q8YXQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)