Reviewed, UniProtKB/Swiss-Prot Q8YXQ0 (QUEF_ANASP)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	all1162

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 136	136	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000162948

Sequences

	Sequence		Length	Mass (Da)
	Q8YXQ0-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: FC1D38319CEBE711	FASTA	136	15,533
	10 20 30 40 50 60 MSNSSPETVS QPSQEVKYGE REIAEGQLIT FPNPRVGRRY DINITLPEFT CKCPFSGYPD 70 80 90 100 110 120 FATIYITYVP DERVVELKAL KLYINSYRDR YISHEESANQ ILDDFVAACD PLEANVKADF 130 TPRGNVHTVV EVRHTK

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB73119.1.
PIR	AG1951.
RefSeq	NP_485205.1.
3D structure databases
SMR	Q8YXQ0. Positions 58-127.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YXQ0.
Genome annotation databases
GeneID	1104756.
GenomeReviews	Gene locus all1162 in contig BA000019_GR.
KEGG	ana:all1162.
NMPDR	fig|103690.1.peg.1472.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG294103.
OMA	RVGRRYD.
Enzyme and pathway databases
BioCyc	NSP103690:ALL1162-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_ANASP

Accession

Primary (citable) accession number: Q8YXQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)