Reviewed, UniProtKB/Swiss-Prot Q8YXJ6 (NADB_ANASP)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	alr1217

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2).
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	L-aspartate oxidase		PRO_0000184377
Regions
	Nucleotide binding	32 – 46	15	FAD Potential
Sites
	Active site	252	1	By similarity
	Active site	271	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YXJ6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0A116B14EAFCBBD8	FASTA	578	63,173
	10 20 30 40 50 60 MITHYSLPIT RYPLPRFLTL SQIEFPSQFD VLVVGAGAAG LYTALCLPES LRVGLITKET 70 80 90 100 110 120 VALSASDWAQ GGIAAAIAPN DSPSLHIEDT IQAGAGLCDV AAVEFLAQQA PKCIESLVNL 130 140 150 160 170 180 GVAFDRHGQA LALTLEAAHS RNRVLHAADT TGREVTTTLT DQVLRRPNIQ VIQQALALSL 190 200 210 220 230 240 WLEPETGHCQ GISLFYQGAI TWVRAGAVVL ATGGGGQVFA QTTNPAVSTG DGVAIAWRAG 250 260 270 280 290 300 AILRDLEFVQ FHPTALTKPG ADRFLISEAV RGEGAHLVDN EGRRFAFDYH PAGELAPRDV 310 320 330 340 350 360 VSRAIFSHLQ RTAVDPATAH VWLDMRPIPE DKIRLRFPNI VKVCQRWGID VFREPIPVAP 370 380 390 400 410 420 AAHYWMGGIA TNLKNQTNIP GLYAVGETAS TGVHGANRLA SNSLLECIVF GAQLANLTPA 430 440 450 460 470 480 DLIQHTETPA LPTREFKIDA SEWQHQQSQL AILREKLPRL VWQSAGICRE QSTLSVAIAT 490 500 510 520 530 540 VKSWQQDFAA LPLSQFLLSL QPNEPVSCNF SDTDQQLRLW AETGNLLDVA YLILKSAAFR 550 560 570 IESRGGHFRL DYPHSNPDWQ VHSLVQKHQW WQSPIMKS

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73174.1.
PIR	AF1958.
RefSeq	NP_485260.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	1104812.
GenomeReviews	Gene locus alr1217 in contig BA000019_GR.
KEGG	ana:alr1217.
NMPDR	fig|103690.1.peg.1527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YXJ6.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1217-MON.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_ANASP

Accession

Primary (citable) accession number: Q8YXJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)