Reviewed, UniProtKB/Swiss-Prot Q8YXG5 (PDXH_ANASP)

Names and origin

Protein names

Recommended name:
    Pyridoxine/pyridoxamine 5'-phosphate oxidase
    EC=1.4.3.5
Alternative name(s):
    PNP/PMP oxidase
      Short name=PNPOx
    Pyridoxal 5'-phosphate synthase

Gene names

Name:	pdxH
Ordered Locus Names:	all1248

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) By similarity.
Catalytic activity	Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2. HAMAP MF_01629 Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2. HAMAP MF_01629
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. HAMAP MF_01629 Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the pyridoxamine 5'-phosphate oxidase family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: HAMAP pyridoxamine-phosphate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Pyridoxine/pyridoxamine 5'-phosphate oxidase HAMAP MF_01629		PRO_0000167679
Regions
	Nucleotide binding	77 – 78	2	FMN By similarity
	Nucleotide binding	141 – 142	2	FMN By similarity
	Region	9 – 12	4	Substrate binding By similarity
	Region	192 – 194	3	Substrate binding By similarity
Sites
	Binding site	62	1	FMN By similarity
	Binding site	65	1	FMN; via amide nitrogen By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	84	1	FMN By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YXG5-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 39CED2EFE2888304	FASTA	214	25,064
	10 20 30 40 50 60 MDRTIADLRK DYTLEGLSEI EVDPNPFIQF KKWFEQALAA QLPEPNAMTI ATSTPDGQPS 70 80 90 100 110 120 ARMVLLKDFD EQGFVFFTNY NSRKGQELAE NPQAALVFWW AELERQVRIS GRVEKVSESE 130 140 150 160 170 180 SDYYFYSRPA NSRLGAWVSN QSEIIASREV LEQRMQEFQH KYENQEIPRP AHWGGLRVIP 190 200 210 SEIEFWQGRS SRLHDRLLYT LLNDHSWEIH RLSP

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB73205.1.
PIR	AE1962.
RefSeq	NP_485291.1.
3D structure databases
HSSP	HSSP built from PDB template 1NRG based on UniProtKB Q9NVS9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8YXG5.
Genome annotation databases
GeneID	1104843.
GenomeReviews	Gene locus all1248 in contig BA000019_GR.
KEGG	ana:all1248.
NMPDR	fig|103690.1.peg.1558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YXG5.
OMA	FTFFTNY.
Enzyme and pathway databases
BioCyc	NSP103690:ALL1248-MON.
Family and domain databases
HAMAP	MF_01629. [Tree]
InterPro	IPR000659. Pyridoxamine_oxidase. IPR019740. Pyridoxamine_oxidase_CS. IPR019576. Pyridoxamine_oxidase_dimer_C. IPR011576. PyridoxamineP_oxidase_FMN-bd. IPR012349. Split_barrel_FMN_bd. [Graphical view]
Gene3D	G3DSA:2.30.110.10. PNPOx_FMN_bd. 1 hit.
PANTHER	PTHR10851. Pyridox_oxidase. 1 hit.
Pfam	PF10590. PNPOx_C. 1 hit. PF01243. Pyridox_oxidase. 1 hit. [Graphical view]
ProDom	PD006312. Pyridox_oxidase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00558. pdxH. 1 hit.
PROSITE	PS01064. PYRIDOX_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXH_ANASP

Accession

Primary (citable) accession number: Q8YXG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)